U2OS, HCT116, and HEK293T cells were obtained from ATCC and cultured in D-MEM medium (U2OS and HEK293T) or RPMI 1640 (HCT116) supplemented with 10% fetal bovine serum, l -glutamine, penicillin, and streptomycin. OCI-AML3 cells were purchased from DSMZ GmbH and cultured in a D-MEM medium (PAN-Biotech) containing 20% FBS, l -glutamine, penicillin, and streptomycin. Cells were routinely tested for mycoplasma infection with a PCR-based method. For SILAC labeling, cells were cultured in media containing either l -arginine and l -lysine, l -arginine [13C6], and l -lysine [2H4] or l -arginine [13C615N4] and l -lysine [13C6-15N2] (Cambridge Isotope Laboratories)79 . All cells were cultured at 37 °C in a humidified incubator containing 5% CO2.
L arginine 13c615n4 and l lysine 13c6 15n2
Manufactured by Cambridge Isotopes
L-arginine [13C615N4] and l-lysine [13C6-15N2] are stable isotope-labeled amino acids. L-arginine [13C615N4] contains six 13C atoms and four 15N atoms, while l-lysine [13C6-15N2] contains six 13C atoms and two 15N atoms. These labeled amino acids are used as tracers in various research and analytical applications.
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Lab products found in correlation
L arginine, by Cambridge Isotopes (2 mentions)
L lysine, by Cambridge Isotopes (2 mentions)
Dmem medium, by American Type Culture Collection (1 mentions)
13c6 l arginine, by Cambridge Isotopes (1 mentions)
Oci aml3 cells, by Leibniz Institute DSMZ (1 mentions)
Dmem medium, by PAN Biotech (1 mentions)
L arginine 13c6 and l lysine 2h4, by Cambridge Isotopes (1 mentions)
2 protocols using l arginine 13c615n4 and l lysine 13c6 15n2
1
SILAC Labeling of Cell Lines
2
SILAC Labeling of U2OS and HEK293T Cells
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U2OS and HEK293T cells were obtained from ATCC and cultured in d -MEM medium supplemented with 10% fetal bovine serum, l -glutamine, penicillin, and streptomycin. Cells were routinely tested for mycoplasma infection with a PCR-based method. For SILAC labeling, cells were cultured in media containing either l -arginine and l -lysine, l -arginine [13C6] and l -lysine [2H4] or l -arginine [13C615N4] and l -lysine [13C6-15N2] (Cambridge Isotope Laboratories) as described previously (62 (link)). All cells were cultured at 37°C in a humidified incubator containing 5% CO2.
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