Doxorubicin (Solarbio, Beijing, China), formaldehyde (Solarbio, Beijing, China), heparin sodium (Solarbio, Beijing, China), acetonitrile (Merck, Germany), formic acid (CNW, Germany), BCA Kit (Solarbio, Shangahi, China), Tris (Sigma, USA), SDS (Bio-Rad, USA), phenylmethylsulfonyl fluoride (Solarbio, Beijing), phosphatase inhibitor (Solarbio, Beijing, China), RIPA buffer (Solarbio, Beijing, China), PTP1B (Abcam, USA), IRS-1, P-IRS, HK2, HIF-1α (Cell Signaling Technology, Inc., USA), Nrf2 (Abcam, USA), NH4HCO3 (Sigma, USA), High pH Reversed-Phase Peptide Fractionation Kit (Pierce, USA), TMT 6/10 plex Isobaric Label Reagent (Thermo, USA) were used.
P irs
Manufactured by Cell Signaling Technology
Sourced in United States
The P-IRS product is a phosphorylation-specific antibody that recognizes the phosphorylated form of the Insulin Receptor Substrate (IRS) protein. This antibody can be used to detect the activation of the insulin signaling pathway in various cell types and tissues.
Automatically generated - may contain errors
Lab products found in correlation
P akt, by Cell Signaling Technology (3 mentions)
β actin, by Cell Signaling Technology (2 mentions)
Phosphatase inhibitor, by Solarbio (1 mentions)
Bca kit, by Solarbio (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Sodium dodecyl sulfate (sds), by Bio-Rad (1 mentions)
Ptp1b, by Abcam (1 mentions)
Irs 1, by Cell Signaling Technology (1 mentions)
Formaldehyde, by Solarbio (1 mentions)
Ripa buffer, by Solarbio (1 mentions)
Nh4hco3, by Merck Group (1 mentions)
Doxorubicin, by Solarbio (1 mentions)
Tmt 6 10 plex isobaric label reagent, by Thermo Fisher Scientific (1 mentions)
Hif 1α, by Cell Signaling Technology (1 mentions)
Heparin sodium, by Solarbio (1 mentions)
Phenylmethylsulfonyl fluoride, by Solarbio (1 mentions)
Gel pro image analysis software, by Media Cybernetics (1 mentions)
Metaphosphoric acid, by Merck Group (1 mentions)
Thiobarbituric acid, by Merck Group (1 mentions)
Phosphoric acid, by Merck Group (1 mentions)
4 protocols using p irs
1
Quantitative Proteomic Analysis of Doxorubicin-Induced Cellular Changes
2
Western Blot Analysis of Protein Signaling
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Total proteins were extracted and quantified from the cell or from the retroperitoneal and epididymal adipose tissue. Then, 30–50 μg protein was isolated from each sample using 10% SDS-PAGE (stacking gel, 50 V; separating gel, 100 V), and transferred into the nitrocellulose membrane (100 V, 75 min). Membranes were blocked and incubated with primary antibodies followed by secondary antibodies. Horseradish peroxidase-labeled secondary antibodies were detected by chemiluminescence and the grayscale of the protein bands was analyzed with Gel-pro Image Analysis Software (Media Cybernetics, Rockville, MD, USA). The primary antibodies against p-IRS (Cell Signaling Technology, Danvers, MA, USA), p-Akt (Cell Signaling Technology, Danvers, MA, USA), and beta-Actin (Cell Signaling Technology, Danvers, MA, USA) were used in this study.
3
Mori Cortex radicis Cultivation and Analysis
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Mori Cortex radicis (MCR) was cultivated in Andong (Kyeongbuk) and dried MCR was purchased in Kyeongdong Market (Seoul, Korea) in April 2017. Metaphosphoric acid, thiobarbituric acid, phosphoric acid, 5,5’-dithiobis-(2-nitrobenzoic acid), and sodium phosphate were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Primary antibodies for P-IRS, IRS, PI3K, p-Akt, Akt, Bax, Tau, GLUT4, AChE, and β-actin were purchased from Cell Signaling Technology (Danvers, MA, USA), and p-Tau and Bcl-2 were acquired from Santa Cruz Biotechnology Inc. (Santacruz, CA, USA). Horseradish peroxidase (HRP)-conjugated anti-rabbit and anti-mouse (secondary antibody) were purchased from Bio-Rad Co. (Bio-Rad, Hercules, CA, USA). Enhanced chemiluminescence (ECL) solution was obtained from Amersham Life Science Corp. (Arlington Heights, IL, USA), and other chemicals were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA).
4
Buformin Effects on Cell Signaling
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Buformin was purchased from Wako Pure Chemical Industries (Osaka, Japan). Primary antibodies against AMPK, p-AMPK, mTOR, p-mTOR, p70S6K, p-p70S6K, 4EBP1, p-4EBP1, IRS, p-IRS, IGF1R, p-IGF1R, p-erbB-2, Akt, p-Akt, p-Erk1/2, p-Stat3, p-ER, β-catenin, Oct4A, and Notch were purchased from Cell Signaling (Danvers, MA). Antibodies against IGF1Rα/β, Erk, Stat3, ER, Cyclin D1, and β-actin were ordered from Santa Cruz Biotechnology (Santa Cruz, CA). erbB-2 and active β-catenin antibodies were purchased from EMD Millipore (Billerica, CA).
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