We collected whole blood samples and centrifuged them at 5000 rpm for 15 min at 4 °C. Plasma levels of triglycerides (T7532, Pointe scientific Inc, Canton, MI), high density lipoprotein (HDL, H7511, Pointe scientific Inc), total cholesterol (C7510, Pointe scientific Inc), aspartate transaminase (AST, EASTR-100, BioAssay Systems, Hayward, CA) and alanine transaminase (ALT, EALT-100, BioAssay Systems) were measured with a colorimetric method as followed in the manufacturer’s instructions. Hemolyzed samples were eliminated for AST or ALT detection. ELISA determined plasma IL6 (88–7064, Invitrogen) and MCP-1 (88–7391, Invitrogen) levels.
H7511
Manufactured by Horiba
The H7511 is a compact and versatile pH/mV/Temperature meter from Horiba. It features a large LCD display, automatic temperature compensation, and a data-hold function. The device is designed to provide accurate and reliable pH, mV, and temperature measurements for various applications.
Automatically generated - may contain errors
2 protocols using h7511
1
Blood Biomarker Profiling Protocol
2
Plasma Lipid Profile Analysis
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The commercially available colorimetric assays were used to determine the plasma level of glucose, triglycerides, total cholesterol, and HDL (cat. no G7521, T7532, C7510, and H7511, respectively; Pointe Scientific, Warsaw, Poland). The concentration of the low-density lipoprotein (LDL) fraction was calculated using the following formula: LDL = total cholesterol-HDL (triglycerides/5). Detection limits were 1 mg/dL for glucose, 5 mg/dL for triglycerides, 3 mg/dL for total cholesterol, and 3 mg/dL for HDL. The intra-assay and inter-assay coefficients of variation for glucose, triglycerides, total cholesterol, and HDL were 1.1% and 2.5%, 1.62% and 1.92%, 1.32% and 1.93%, and 4.7% and 5.1%, respectively. All analyses were performed in duplicate.
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