Spheroids from oral mucosa-, skin-, and CBDC-derived cells were transferred into a new regular culture dish. After these cultures reached 50–60% confluence, the medium was changed to neurogenic induction medium. The medium used for neural cell differentiation was αMEM supplemented with l -glutamine, phenol red (Wako), 50 ng/ml nerve growth factor, 50 ng/ml brain-derived neurotrophic factor, 10 ng/ml NT-3 (all from Peprotech), 10% FBS, 100 U/ml penicillin, 100 μg/ml streptomycin, and 0.25 μg/ml amphotericin B (Biological Industries). For Schwann cell differentiation, the medium used was αMEM supplemented with l -glutamine, phenol red (Wako), 5 μM forskolin (Sigma), 50 ng/ml heregulin-1β (Peprotech), 2% v/v N2 supplement (Invitrogen), 10% FBS, 100 U/ml penicillin, 100 μg/ml streptomycin, and 0.25 μg/ml amphotericin B (Biological Industries). The cells were differentiated for 1 or 2 weeks, and 50% of the medium was changed every 2 days.
Phenol red
Manufactured by Fujifilm
Sourced in Japan
Phenol red is a chemical compound used in laboratory equipment. It functions as a pH indicator, changing color in response to changes in acidity or alkalinity of a solution.
Automatically generated - may contain errors
Lab products found in correlation
L glutamine, by Fujifilm (31 mentions)
Sodium pyruvate, by Fujifilm (12 mentions)
Hepes, by Fujifilm (5 mentions)
Rpmi 1640, by Fujifilm (4 mentions)
Fetal bovine serum (fbs), by Biowest (3 mentions)
Dmem ham s f 12, by Fujifilm (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Penicillin streptomycin, by Fujifilm (3 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Biosera (2 mentions)
Dmem high glucose, by Fujifilm (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin p s, by Nacalai Tesque (2 mentions)
Endothelial cell growth medium, by PromoCell (2 mentions)
Horse serum, by Thermo Fisher Scientific (2 mentions)
Sh sy5y cell, by American Type Culture Collection (2 mentions)
Lenti x 293t cell line hek293t, by Takara Bio (2 mentions)
D mem ham s f 12 with l glutamine and phenol red, by Fujifilm (2 mentions)
N2 supplement, by Thermo Fisher Scientific (1 mentions)
Nerve growth factor (ngf), by Thermo Fisher Scientific (1 mentions)
45 protocols using phenol red
1
Differentiation of Oral, Skin, and CBDC Cells
2
Culturing Human Mesothelioma Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human mesothelioma cell lines: NCI-H28, NCI-H2052, NCI-H2452, and MSTO-211H, and the simian virus 40 (SV40)-transformed mesothelial cell line MeT-5A were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). The other human mesothelioma cell lines used in this study; Y-MESO-8D, Y-MESO-14, and Y-MESO-27, were the gifts from Dr. Y. Sekido, Aichi Cancer Center Research Institute (Aichi, Japan). All mesothelioma cells were maintained in RPMI-1640 medium with L-glutamine and Phenol Red (Fujifilm-Wako, Tokyo, Japan) supplemented with 10% fetal bovine serum (FBS) (EU origin; Biosera) at 37 °C under a 5% CO2 atmosphere.
3
Cell Culture with Fetal Bovine Serum
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fetal bovine serum (FBS) was obtained from Biowest (Nuaille, France). DMEM (high glucose) with L-glutamine, phenol red, and sodium pyruvate was obtained from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Plastic dishes were obtained from TPP (Trasadingen, Switzerland). All other materials used were of the highest commercial grade.
4
Cultivation of VAT-39 Human HAC Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
VAT-39 human HAC cells from the ampulla of Vater [33 (link)] were maintained in Dulbecco’s Modified Eagle’s Medium with L-glutamine and phenol red (Fujifilm Wako Pure Chemical Corporation, Osaka, Japan). The medium was supplemented with 10% (v/v) heat-inactivated fetal bovine serum and penicillin-streptomycin-amphotericin B in a humidified atmosphere containing 5% CO2 at 37°C.
5
Cytotoxicity of Green Tea Catechins on SH-SY5Y Neuroblastoma Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human SH-SY5Y neuroblastoma cells (ACTT, CRL-2266) were plated in a 100-mm flask and cultured in D -MEM/Ham's F-12 with L -glutamine, phenol red, HEPES and sodium pyruvate (Wako Pure Chemical Industries, Ltd., Osaka, Japan), containing 10% fetal bovine serum (Mediatech, Inc., Tokyo, Japan) and a mixture of 1% penicillin-streptomycin (Nacalai Tesque, Inc. Kyoto, Japan). The cell culture was incubated at 37 °C under 5% CO2 for 48 h in an incubator (Hirasawa, Tokyo, Japan). After reaching 70–80% confluence, cells were Trypsinized (Trypsin, Gibco Life Technologies, NY, USA), and then cells were plated as 1×105 cells/mL in a 24-well plate (500 µL of cell suspension/well). EGCG, EGC and GA dissolved in 0.01% DMSO were added to the culture medium to make a final concentration of 0.01–1.0 µM in triplicate for each concentration. Plates were incubated for 48 h. To determine cell viability, a cell suspension was prepared by Trypsinization as indicated above and a 1:1 mixture of the cell suspension with 0.4% trypan blue dye (Cosmo Bio Co. Ltd., Tokyo, Japan) was prepared. Cells were counted with a TC10TM Automated Cell Counter (Bio-Rad, CA, USA). The experiment of each catechins was carried out twice.
6
Cultivation of Human Oral Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human HSC-3 OSCC cell line was obtained from the National Institute of Biomedical Innovation (Osaka, Japan). HSC-3 cells were cultured in high glucose Dulbecco's modified Eagle's medium (DMEM) supplemented with L-glutamine and Phenol Red (Wako Pure Chemical Industries, Ltd., Osaka, Japan), 10% fetal bovine serum and 1% penicillin/streptomycin (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) at 37°C in a humidified atmosphere of 5% CO2. Cells were incubated in DMEM for 24–48 h prior to treatment. Early passages of cells (between 2 and 10 passages from the stage of primary culture) were used in the current study.
7
COS7 Cells Imaging on CaF2 Substrate
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
COS7 cells are cultured on a CaF2 substrate with a thickness of 500 μm in high glucose Dulbecco’s modified eagle medium with L-glutamine, phenol red, and HEPES (FUJIFILM Wako) supplemented with 10% fetal bovine serum (Cosmo Bio) and 1% penicillin-streptomycin-L-glutamine solution (FUJIFILM Wako) at 37 °C in 5% CO2, and are sandwiched with another CaF2 substrate before imaging. For live-cell imaging in D2O environment (Fig. S4 ), the medium is replaced by D2O-based PBS. Note that MIP imaging with glass substrates is feasible in the spectral range observed in this work.
8
FRET-labeled HIV-1 Virion Production
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Adherent HEK293T and TZM-bl cells were cultured in Dulbecco’s Modified Eagle’s Medium (Nacalai Tesque) containing 10% Fetal Bovine Serum and 1% Penicillin Streptomycin Glutamine (Invitrogen) (D10) at 37°C with 5% CO2.
FRET labeled virions were produced by co-transfecting HEK293T cells (3.5 × 106 cells/10 cm dish) with the pHIV-1 Gag-iFRET or iFRETΔPro together with the pNL4-3 or pNL4-3ΔPro parental plasmid respectively at a 1:1, 1:10, or 1:20 ratio using a polyethylenimine transfection reagent (GE Healthcare). The culture medium was replaced with fresh D10 with or without Darunavir (Sigma Aldrich) at a final concentration of 0.1, 1.0, 10, 20, 500, or 1000 nM 3.5 h after transfection. The virus-containing supernatant was harvested 24 h after the medium change, filtered through 0.45 μm pore size sterile polyvinylidene difluoride (PVDF, Millipore) membrane, and concentrated up to 20-fold by ultracentrifugation through a 20% sucrose cushion at 25,000 rpm (112,499 g) for 90 min at 4°C (CP65; Hitachi Koki Co., Ltd.). The virus pellet was resuspended in 500 μl Hank’s Balanced Salt Solution (HBSS) (–) without phenol red (Wako).
FRET labeled virions were produced by co-transfecting HEK293T cells (3.5 × 106 cells/10 cm dish) with the pHIV-1 Gag-iFRET or iFRETΔPro together with the pNL4-3 or pNL4-3ΔPro parental plasmid respectively at a 1:1, 1:10, or 1:20 ratio using a polyethylenimine transfection reagent (GE Healthcare). The culture medium was replaced with fresh D10 with or without Darunavir (Sigma Aldrich) at a final concentration of 0.1, 1.0, 10, 20, 500, or 1000 nM 3.5 h after transfection. The virus-containing supernatant was harvested 24 h after the medium change, filtered through 0.45 μm pore size sterile polyvinylidene difluoride (PVDF, Millipore) membrane, and concentrated up to 20-fold by ultracentrifugation through a 20% sucrose cushion at 25,000 rpm (112,499 g) for 90 min at 4°C (CP65; Hitachi Koki Co., Ltd.). The virus pellet was resuspended in 500 μl Hank’s Balanced Salt Solution (HBSS) (–) without phenol red (Wako).
9
Culturing Oral Mucosal Epithelium
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The separated oral mucosal epithelium was cultured in DMEM/Ham’s F-12 containing L-glutamine, Phenol Red, HEPES, and sodium pyruvate (Wako, Osaka, Japan) supplemented with B27, EGF and penicillin-streptomycin-amphotericin b Suspension (Wako, Osaka, Japan) in a CO2 incubator at 37 °C for 3 weeks. The medium was exchanged after the first week, every other day during the second week, and every day during the third week. In order to promote tissue adhesion, sufficient medium was added to completely cover the surface of the mucosal tissue for the first week.
10
Ex vivo Absorption of bAA Fibrils
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An ex vivo experiment was performed to determine the effects of exposing
PP to high concentrations of bAA, and to compare the absorption of bAA with other
materials. All mice were euthanized by deep anesthesia and the intestinal tracts were
collected. Both ends of the PP-containing intestine were ligated approximately 5 mm before
and after each PP. bAA fibrils (54 µg/20 µl) or α-syn fibrils (40 µg/20 µl) were
inoculated into each intestinal lumen, with PBS used as an inoculation control. After
inoculation, each intestine fragment was cultured in D-MEM / Ham’s F-12 with L-Glutamine,
Phenol Red, HEPES, and Sodium Pyruvate (Wako, Osaka, Japan) at 37°C and sampled overtime
for up to 6 hr.
PP to high concentrations of bAA, and to compare the absorption of bAA with other
materials. All mice were euthanized by deep anesthesia and the intestinal tracts were
collected. Both ends of the PP-containing intestine were ligated approximately 5 mm before
and after each PP. bAA fibrils (54 µg/20 µl) or α-syn fibrils (40 µg/20 µl) were
inoculated into each intestinal lumen, with PBS used as an inoculation control. After
inoculation, each intestine fragment was cultured in D-MEM / Ham’s F-12 with L-Glutamine,
Phenol Red, HEPES, and Sodium Pyruvate (Wako, Osaka, Japan) at 37°C and sampled overtime
for up to 6 hr.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!