Rpmi 1640 modified medium

Bloodstream Trypanosoma brucei
brucei Lister 427 was cultured in Hirumi’s modified
Iscove’s medium (HMI-9), supplemented with 10% heat-inactivated
FBS, at 37 °C and 5% CO₂ in T-25 vented flask (Corning).
MRC5-SV2 cell line (SV40-transformed human lung fibroblast cell
line) was cultured in DMEM medium supplemented with 10% FBS at 37
°C and 5% CO₂ in T-75 vented flask (Corning).
The T. cruzi Tulahuen C4 strain, expressing the
β-galactosidase gene (LacZ) and L6 rat skeletal muscle cells,
used as host cells, were cultured in RPMI-1640 supplemented with 10%
iFBS, 2 mM l-glutamine, 100 U/mL penicillin, and 100 μg/mL
streptomycin at 37 °C and 5% CO₂.
Leishmania
donovani MHOM/ET/67/HU3 cells with
the luciferase gene integrated into the parasite genome were grown
at 28 °C in RPMI 1640-modified medium (Invitrogen) supplemented
with 20% FBS with 100 mg/mL of hygromycin B.^{37 (link)}The human myelomonocytic cell line THP-1 was grown at 37 °C
and 5% CO₂ in RPMI-1640 supplemented with 10% iFBS, 2 mM
glutamate, 100 U/mL penicillin, and 100 mg/mL streptomycin. 3 ×10⁴ THP-1 cells per well in 96-well plates were differentiated
to macrophages with 20 ng/mL of PMA treatment for 48 h followed by
24 h of culture in fresh medium.
Maintenance of the Schistosoma mansoni life cycle
(NMRI isolate), the preparation of adult worms (≥42-days-old),
and phenotypic screens with test compounds were as described.^{38 (link)−40 (link)}