Zetapals instrument

Manufactured by Brookhaven Instruments

Sourced in United States

The ZetaPALS instrument from Brookhaven Instruments is a versatile tool used for measuring the zeta potential and size of particles in a wide range of samples. It utilizes the technique of electrophoretic light scattering to determine the surface charge and hydrodynamic size of particles suspended in a liquid medium.

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Lab products found in correlation

Dynapro plate reader, by Wyatt Technology (3 mentions) Ez link sulfo nhs lc biotin, by Thermo Fisher Scientific (2 mentions) Vertex 70 ftir spectrometer, by Bruker (2 mentions) Whatman, by Cytiva (2 mentions) Bi 90 particle sizer, by Brookhaven Instruments (2 mentions) Jem 2100 tem, by JEOL (1 mentions) Electrophoresis system, by Bio-Rad (1 mentions) 4 2 hydroxyethyl 1 piperazineethanesulfonic acid hepes, by Merck Group (1 mentions) Rneasy kit, by Qiagen (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Gold 3 chloride trihydrate, by Merck Group (1 mentions) Sodium citrate tribasic dihydrate, by Merck Group (1 mentions) Cary 500, by Agilent Technologies (1 mentions) 1 octadecanethiol, by Merck Group (1 mentions) 1 palmitoyl 2 hydroxy sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phospho l serine sodium salt pops, by Avanti Polar Lipids (1 mentions) Xrd 6000, by Shimadzu (1 mentions) Ns300, by Malvern Panalytical (1 mentions) Legendx1, by Thermo Fisher Scientific (1 mentions) Genequant 1300 spectrophotometer, by GE Healthcare (1 mentions)

Spelling variants of this product

ZetaPALS (173 citations) ZetaPals dynamic light scattering (DLS) instrument (4 citations)

44 protocols using zetapals instrument

Physicochemical Characterization of Nanoparticles

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Physicochemical characteristics of the NPs were determined using a Zeta PALS instrument (Brookhaven Instruments, Austin, TX, USA). Dynamic light scattering (DLS) was employed to determine the NPs diameter and polydispersity index. The samples were prepared by diluting the NP formulation with PBS (pH 7.4) to a count of 300–500 and sonicated for 5 mins before the readings being taken at 25±1°C. All the readings were taken in triplicate.

Khan M.W., Zhao P., Khan A., Raza F., Raza S.M., Sarfraz M., Chen Y., Li M., Yang T., Ma X, & Xiang G. (2019). Synergism of cisplatin-oleanolic acid co-loaded calcium carbonate nanoparticles on hepatocellular carcinoma cells for enhanced apoptosis and reduced hepatotoxicity. International Journal of Nanomedicine, 14, 3753-3771.

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Formulation and Characterization of CRT-Loaded Lipid Nanoparticles

Cited 1 time

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Full-length clone DNA of human CRT cloned into pCMV3 vector was used (HG13539-ACR, Sino Biological Inc., Wayne, PA, USA). For CRT-NP synthesis, a lipid film was hydrated in 10 mM HEPES buffer (pH 7.4) at 55°C, and the lipid suspension was then extruded five times through filters of 200 nm pore size to yield homogeneous liposomes 34 (link). Next, a one-step method for loading the plasmid was developed by adding the pDNA solution in the liposomes vial (1:10, wt/wt), gently mixing them by pipetting, and incubating at room temperature for 30 min. The resultant CRT-NPs were characterized for plasmid encapsulation by the gel retardation assay in 1% agarose pre-cast gels containing ethidium bromide. A control sample of free pCRT as well as blank NPs were loaded onto the gels and the gels were run at 80 V on a Bio-Rad electrophoresis system. DNA dose was 0.2 µg per lane. Approximately 60 minutes after beginning the run, the gels were observed for plasmid migration. The CRT-NPs were also characterized in physiological buffers at room temperature by size (z-average) and zeta-potential using dynamic light scattering (DLS) with a Brookhaven ZetaPALS instrument (Holtsville, NY, USA). Furthermore, transmission electron microscopy (TEM) as previously published was performed to assess the morphology of CRT-NP using the JEOL JEM-2100 TEM (JEOL USA, Peabody, MA, USA) 26 (link).

Sethuraman S.N., Singh M.P., Patil G., Li S., Fiering S., Hoopes P.J., Guha C., Malayer J, & Ranjan A. (2020). Novel calreticulin-nanoparticle in combination with focused ultrasound induces immunogenic cell death in melanoma to enhance antitumor immunity. Theranostics, 10(8), 3397-3412.

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Functionalized Au Nanoparticles for RNA Extraction

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Gold(III) chloride trihydrate (HAuCl₄·3H₂O, ≥99.9%), sodium citrate tribasic dihydrate (Na₃Ct.2H₂O, ≥99%), 1-octadecanethiol (98%, C₁₈SH), poly(allylamine hydrochloride), (PAH, M.W. 15,000 g/mole), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) were obtained from Sigma Aldrich and were used as received. 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (sodium salt) (POPS), 1-palmitoyl-2-hydroxy-sn-glycero-3-phosphocholine (LPC) were obtained from Avanti Polar Lipids and were used as received. Trizol (Invitrogen) and RNeasy kit (Qiagen) were used in the extraction of RNA. Ultrapure deionized water (17.9 MΩ, Barnstead NANOpure II) was used for all solution preparations. Glassware was cleaned with aqua regia and rinsed thoroughly before use. Absorption spectra of Au NPs (Figure S1) were taken on a Cary 500 scan UV-vis-NIR spectrophotometer and absorption spectra of RNA were taken on Nanodrop 1000. Zeta potential and dynamic light scattering measurements were performed on a Brookhaven Zeta PALS instrument.

Grzincic E.M., Yang J.A., Drnevich J., Falagan-Lotsch P, & Murphy C.J. (2015). Global Transcriptomic Analysis of Model Human Cell Lines Exposed to Surface-Modified Gold Nanoparticles: The Effect of Surface Chemistry. Nanoscale, 7(4), 1349-1362.

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Characterizing CNC Suspension Zeta Potentials

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A ZetaPALS instrument (Brookhaven Instruments, Holtsville, NY, USA) was used to characterize the zeta potentials of the CNC suspensions. At least five measurements were performed and the data were averaged, all measurements were taken at 25 °C.

Wu Q., Li X., Li Q., Wang S, & Luo Y. (2019). Estimation of Aspect Ratio of Cellulose Nanocrystals by Viscosity Measurement: Influence of Aspect Ratio Distribution and Ionic Strength. Polymers, 11(5), 781.

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Biotinylation and Characterization of Purified Aquasomes

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Purified Ana GVs were biotinylated using a 10⁵ molar excess of EZ-Link Sulfo-NHS-LC-biotin (Thermo Scientific, Rockford, IL) for 4 hours in PBS buffer. The excess biotin was removed by two rounds of overnight dialysis in PBS buffer. Biotinylated or control GVs at OD_500,PS = 10 were incubated with streptavidin (Geno Technology, St. Louis, MO) at the ratio specific to each experiment for 30 minutes at room temperature before loading into MRI phantom. Dynamic light scattering measurements were performed using a Zeta-PALS instrument (Brookhaven Instruments, Hotsville, NY) at a concentration equivalent to OD_500,PS = 0.2 in PBS.

Lu G.J., Farhadi A., Szablowski J.O., Lee-Gosselin A., Barnes S.R., Lakshmanan A., Bourdeau R.W, & Shapiro M.G. (2018). Acoustically modulated magnetic resonance imaging of gas-filled protein nanostructures. Nature materials, 17(5), 456-463.

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Dynamic Light Scattering Catalyst Characterization

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DLS experiments were conducted on a Brookhaven ZETAPALS instrument. DLS measurements were obtained both before and after heating the catalyst solutions. The autocorrelation functions were analyzed using the Multimodal Size Distribution algorithm of the accompanying software, where the viscosity of the solutions was assumed to equal that of water.

Norton A.M., Nguyen H., Xiao N.L, & Vlachos D.G. (2018). Direct speciation methods to quantify catalytically active species of AlCl3 in glucose isomerization. RSC Advances, 8(31), 17101-17109.

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Characterization of Nanoparticles by Analytical Techniques

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FTIR analysis was achieved using an FTIR spectrometer (8400 S, Shimadzu, Japan) with attenuated total reflection mode, spectral range of 4000–400 cm⁻¹, and a resolution of 4 cm⁻¹. Measurement of the crystalline status of the prepared samples was performed by an X-ray diffractometer machine (XRD-6000, Shimadzu, Japan). A Cu Kα incident beam (λ = 1.542 A°) at 2θ = 20°− 60° was employed to obtain the diffraction patterns. X ray tubes had a voltage of 45 kV and a current of 30 mA. Field Emission Scanning Electron Microscopic (FESEM) analysis was conducted using MIRA 3 TESCAN (Brno -Czech Republic) coupled to an EDAX Team (EDAX, Mahwah, NJ 07430, USA) to examine the shape and presence of the prepared nanoparticles. Transmission electron microscope (TEM; Tecnai G2 20 S-TWIN, China) was applied to examine the shapes of the prepared nanoparticles. Zeta potentials and particle size were measured by Brookhaven Zeta PALS instrument (Milton Keynes, UK) to characterize the nanoparticles.

Sulaiman G.M., Waheeb H.M., Jabir M.S., Khazaal S.H., Dewir Y.H, & Naidoo Y. (2020). Hesperidin Loaded on Gold Nanoparticles as a Drug Delivery System for a Successful Biocompatible, Anti-Cancer, Anti-Inflammatory and Phagocytosis Inducer Model. Scientific Reports, 10, 9362.

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Characterization of CeO2 Nanoparticles

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The primary diameter and length of CeO₂ nanomaterials were determined by transmission electron microscopy (TEM), while the suspended particles were used for assessment of hydrodynamic size and surface charge. CeO₂ nanomaterials were dispersed in deionized water by vortexing and bath sonication to yield a stock solution of 5 mg/mL as previously described.⁸ The hydrodynamic sizes of the CeO₂, dispersed in deionized water, DMEM cell culture medium (supplemented with 10 % FBS), and Holtfreter’s medium (supplemented with 100 ug/mL alginate), were determined by high throughput dynamic light scattering (HT-DLS, Dynapro Plate Reader, Wyatt Technology), as described by Ji et al.^{67 (link)} The surface charge of CeO₂ was determined by a ZetaPALS instrument (Brookhaven Instruments, Holtsville, NY). The ζ-potential was derived using the Helmholtz-Smoluchowski equation based on the measurements of the electrophoretic mobility of CeO₂ in deionized water and Holtfreter’s medium.

Lin S., Wang X., Ji Z., Chang C.H., Dong Y., Meng H., Liao Y.P., Wang M., Song T.B., Kohan S., Xia T., Zink J.I., Lin S, & Nel A.E. (2014). Aspect Ratio Plays a Role in the Hazard Potential of CeO2 Nanoparticles in Mouse Lung and Zebrafish Gastrointestinal Tract. ACS nano, 8(5), 4450-4464.

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Preparation and Characterization of Lipid Nanoparticles for mRNA Delivery

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LNPs were prepared using a previously described method with minor modifications^{15 (link)}. Lipids were dissolved in ethanol at a molar ratio of 50:10:38.5:1.5 (SM-102: DSPC: cholesterol: DMG-PEG2000). mRNA was diluted with 6.25 mM sodium acetate buffer (pH 5) to 0.1 mg/mL. mRNA and lipids were combined in a Dolomite micromixer chip at a volume ratio of 3:1 (aqueous:ethanol) and flow rates of 4.5 mL/min (aqueous) and 1.5 mL/min (ethanol). The buffer of the resulting formulation was exchanged for PBS using Amicon Ultra centrifugal filters (100 K NMWL). To prepare DiD’-labeled LNPs, DiD’ (1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindodicarbocyanine, 4-Chlorobenzenesulfonate Salt; ThermoFisher Scientific, Cat#: D7757) was incorporated in the initial lipid mixture at a concentration of 0.05 mg/mL. Size distribution was measured via Nanoparticle Tracking Analysis using a NanoSight NS300 (Malvern), and zeta potential was determined using dynamic light scattering detected with a ZetaPALS instrument (Brookhaven). The particles were diluted 1:100 (v/v) in PBS for size measurements, and 1:33 (v/v) in water for zeta potential quantitation.

Fitzgerald K., Stephan S.B., Ma N., Wu Q.V, & Stephan M.T. (2024). Liquid foam improves potency and safety of gene therapy vectors. Nature Communications, 15, 4523.

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Characterization of Functionalized Gold Nanoparticles

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A UV-vis spectrometer was used to record the UV-vis absorption spectrum of the synthesized AuNPs (15 nm in diameter). A Fourier transform infrared spectrometer (FT-IR, Thermo Fisher Scientific, USA) was used to record the infrared spectrum of MTX-PEG. The morphology of AuNPs, Au @PDA NPs and Au @PDA-PEG-MTX NPs were observed by TEM. The Brookhaven Zeta PALS instrument was used to record dynamic light scattering (DLS) intensity and zeta potential of AuNPs, Au @PDA NPs, Au @PDA-PEG NPs and Au @PDA-PEG-MTX NPs.

Li W., Cao Z., Yu L., Huang Q., Zhu D., Lu C., Lu A, & Liu Y. (2021). Hierarchical drug release designed Au @PDA-PEG-MTX NPs for targeted delivery to breast cancer with combined photothermal-chemotherapy. Journal of Nanobiotechnology, 19, 143.

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