In experiment 2, dry matter of digesta was quantified by weighing digesta samples prior to and after freeze-drying, as described in Passos et al. [50 (link)]. Nitrogen was quantified in ground feed and digesta samples using TruSpec N Nitrogen Determinator (LECO Corp., St. Joseph, MI, USA) to calculate crude protein (method 992.15; [51 ]). Gross energy was quantified in ground feed and digesta samples using a Parr 6200 Calorimeter (Parr Instrument Co., Moline, IL). Ether extract was quantified in ground feed and digesta samples using ether extraction method (method 920.39; [51 ]).
Truspec n nitrogen determinator
Manufactured by Leco
Sourced in United States, Sao Tome and Principe
The TruSpec N Nitrogen Determinator is a laboratory instrument designed to analyze the nitrogen content in a wide variety of samples. It utilizes combustion technology to determine the nitrogen concentration accurately and efficiently.
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using truspec n nitrogen determinator
1
Quantification of Digesta Composition
2
Nutrient Digestibility Determination Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dried digesta and feeds were used to measure the titanium dioxide, GE, EE, and CP. Gross energy was measured using a calorimeter bomb (Parr 6200–Parr instrument company, Moline, IL, USA). The ether extract was measured following AOAC [28 ] (Method 920.39). Nitrogen was measured using TruSpec N Nitrogen Determinator (LECO Corp., St. Joseph, MI, USA) to calculate CP (Method 992.15; AOAC [28 ]).
The AID of the nutrients was calculated using the following equation [29 (link)]:
where TiO2feed and TiO2digesta were the concentrations of titanium dioxide in the diet and the digesta; Nutrientdigesta and Nutrientfeed were the concentrations of nutrients in the digesta and the diet.
The AID of the nutrients was calculated using the following equation [29 (link)]:
where TiO2feed and TiO2digesta were the concentrations of titanium dioxide in the diet and the digesta; Nutrientdigesta and Nutrientfeed were the concentrations of nutrients in the digesta and the diet.
3
Ileal Nutrient Digestibility Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Diets and ileal digesta were stored at −20 °C until being freeze-dried (24D × 48, Virtis, Gardiner, NY, USA). Diet and freeze-dried digesta samples were ground and analyzed for dry matter (Method 934.01, AOAC, 2006 ). Titanium dioxide concentration was measured at the University of Missouri Experiment Station Chemical Laboratory (Columbia, MO, USA). Nitrogen in the feed and digesta samples was quantified using TruSpec N Nitrogen Determinator (LECO Corp., St. Joseph, MI, USA) to calculate crude protein (6.25 × N). Gross energy was determined using a calorimeter (Model 6200, Parr Instrument Company). Samples of feed and ileal digesta were analyzed sequentially for neutral detergent fiber (NDF) and acid detergent fiber (ADF) using the method of Van Soest et al. (1991) (link) in a batch processor (Ankom Technology Corp, Fairport, NY). Apparent ileal digestibility of dry matter (DM), gross energy (GE), crude protein (CP), NDF, and ADF were calculated using titanium concentration in the feed and digesta. The digestibility was calculated with the following equation: where Tifeed represents the titanium concentration in the feed, Tidigesta is the titanium concentration in the ileal digesta, Nfeed represents the nutrient concentration in the feed, and Ndigesta is the nutrient concentration in the ileal digesta.
4
Nutrient Digestibility Determination
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Samples of diets and freeze-dried digesta from the ileum and rectum were analyzed to determine DM (method 934.01; AOAC, 2006 ), EE (method 2003.06; AOAC, 2006 ), GE using a calorimeter (6200, Parr Instrument Company, Moline, IL), CP using a TruSpec N Nitrogen Determinator (LECO Corp., St. Joseph, MI), and titanium dioxide following Myers et al. (2004) (link). The digestibility of DM, GE, EE, and CP was calculated using the equation previously described by Duarte et al. (2019) (link):
With TiO2diet and TiO2digesta denoting the concentrations of titanium dioxide in the diet and in the digesta from either the ileum or rectal; Nutrientdigesta and Nutrientdiet indicating the concentrations of nutrients in the digesta from the ileum or rectum as well as in the diet. The digestibility of nutrients was reported as apparent ileal digestibility (AID ) and apparent total tract digestibility (ATTD ).
With TiO2diet and TiO2digesta denoting the concentrations of titanium dioxide in the diet and in the digesta from either the ileum or rectal; Nutrientdigesta and Nutrientdiet indicating the concentrations of nutrients in the digesta from the ileum or rectum as well as in the diet. The digestibility of nutrients was reported as apparent ileal digestibility (
5
Determining Nutrient Apparent Ileal Digestibility
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The concentration of titanium dioxide of all samples (feed and digesta) was measured according to Myers et al. (2004) (link), to determine the AID of nutrients. Gross energy (GE) content was measured in calorimeter bomb (Parr 6200 – Parr instrument company, Moline, Illinois). The nitrogen content was measured using a TruSpec N Nitrogen Determinator (LECO Corp., St. Joseph, MI) to calculate crude protein (CP, 6.25 × N). The AID of the nutrients was calculated using the equation: in which TiO2diet and TiO2digesta were the concentrations of titanium dioxide in the diet and in the digesta; Nutrientdigesta and Nutrientdiet were the concentrations of nutrient in the digesta and in the diet.
6
Measurement of Nutrient Digestibility Using Titanium Dioxide
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Titanium dioxide was added at an inclusion rate of 0.4% to starter diets to serve as an indigestible marker to determine the AID of nutrients. Ileal digesta were freeze-dried for 48 h (24D 48, Virtis, Gardiner, NY). The concentration of titanium dioxide in the feed and digesta were measured based on the approach of Myers et al. (2004) (link). The feed and digesta samples were used to measure the content of dry matter (DM , method 934.01), crude fiber (method 978.10), and ether extract (EE , method 2003.06) were measured based on AOAC (2006) . Gross energy (GE ) was measured using a bomb calorimeter (Model 6200, Parr Instrument Company, Moline, IL). The nitrogen content was measured using TruSpec N Nitrogen Determinator (LECO CN-2000, LECO Corp., St. Joseph, MI) and the CP concentration was calculated (6.25 × N). The AID of DM, GE, EE, and CP were calculated by using the following function:
Which TiO2feed and TiO2digesta were the measured concentration of titanium dioxide in the feed and in the digesta; Nutrientdigesta and Nutrientfeed were the measured concentration of nutrient in the digesta and in the feed as previously described by Moita et al. (2021) (link).
Which TiO2feed and TiO2digesta were the measured concentration of titanium dioxide in the feed and in the digesta; Nutrientdigesta and Nutrientfeed were the measured concentration of nutrient in the digesta and in the feed as previously described by Moita et al. (2021) (link).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!