FTIR spectra of EBMW formulations 1 and 5 were obtained in a FTIR spectrophotometer from Thermo Scientific (model Nicolet 6700, Madison, WI, USA) coupled with an ATR module (germanium crystal) (Smart Omni Sampler), from 4000 cm−1 to 675 cm−1 (resolution: 4 cm−1, 1024 scans), with Happ–Genzel apodization.
Smart omni sampler
Manufactured by Thermo Fisher Scientific
Sourced in United States
The SMART OMNI-Sampler is a versatile laboratory instrument designed for automated sampling and sample preparation. It performs precise liquid handling tasks such as aspirating, dispensing, and mixing samples across a range of applications.
Automatically generated - may contain errors
Lab products found in correlation
Omnic series software, by Thermo Fisher Scientific (2 mentions)
Nicolet 6700, by Thermo Fisher Scientific (1 mentions)
Is50 ft ir, by Thermo Fisher Scientific (1 mentions)
Nicolet 6700 spectrometer, by Thermo Fisher Scientific (1 mentions)
Originpro 8, by OriginLab (1 mentions)
Omnic 8, by Thermo Fisher Scientific (1 mentions)
5 protocols using smart omni sampler
1
FTIR Analysis of EBMW Formulations
2
FTIR Analysis of FNB-Carrier Interactions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The intermolecular interactions between FNB and the carrier matrix were investigated using an FTIR analysis (iS50 FTIR equipped with a SMART OMNI-Sampler, ThermoFisher Scientific, Waltham, MA, USA). The FNB, HPMC AS LG, PM, and crushed EXT were analyzed from 4000–700 cm−1, at a resolution of 4 cm−1 for % transmittance with 64 scans per run. The background was collected before every run. Weak intermolecular interactions were analyzed and assessed on the OMNICTM series software (ThermoFisher Scientific, Waltham, MA, USA) for the collected spectra.
3
Infrared Spectroscopic Analysis of HTCC-Coated Masks
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The infrared spectra of the HTCC-coated masks were obtained using a Nicolet 6700 spectrometer (Thermo Scientific) with an attenuated total reflection (ATR) Smart Omni sampler accessory, in the range of 670 to 4000 cm−1 and a scanning rate of 4 cm−1.
4
Insulin Secondary Structure Analysis by ATR-FTIR
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Attenuated total reflectance FTIR (ATR-FTIR) spectra were recorded using NicoletTM 8700 Fourier transform infrared spectrometer (Thermo Fisher Scientific) equipped with Smart OMNI-Sampler (diamond crystal). 5 µl of a sample (35 µM human insulin, 1750 μM compound) was spread on the diamond surface. Every sample was measured as a triplicate. Each spectrum represents an average of 128 repetitions recorded at the resolution of 2 cm−1 in the amide I region (1700–1600 cm−1). Recorded spectra were smoothed using OMNIC 8 software (Thermo Fisher Scientific) to achieve the quality of spectra adequate for deconvolution. 11-point Savitzky–Golay filter (10.607 cm−1) followed by 7-point Savitzky–Golay filter (6.750 cm−1) was applied. Spectra were subsequently deconvoluted by the peak analyzer in OriginPro 8 (OriginLab Corporation) (Fig. S8 ). Baseline was subtracted, and the positions of peaks in the amide I region were added manually in correlation with raw data. To correctly assign peak positions to the secondary structures, measured spectra were compared against the published literature43 (link). Gaussian peak function was used to fit the data, and appropriate secondary structures content was obtained by integration of resulting curves.
5
FT-IR Analysis of IND Formulations
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
FT-IR provides insight into the post-processing interactions between different functional groups present on the components. FT-IR analysis was used to investigate the changes in the IND spectrum after each process and the interactions between IND and other components (iS50 FT-IR equipped with a SMART OMNI-Sampler, Nicolet, ThermoFisher Scientific, Waltham, Massachusetts, USA). A sample of 20-25 mg of IND, excipients, PM-I, granules, PM-II, LS printed tablets, and extruded filaments (powdered for the analysis) were dispensed on the sample holder and their % transmittance was measured using a range of 3100-700 cm -1 . The resolution of the test was set to 4 cm -1 with 64 scans per run. To ensure the absence of contamination from previous samples, the cell was cleaned using isopropanol and the background spectrum was collected between each sample. The raw data were translated into spectra which were then investigated for intermolecular interaction, stability, and the solid-state of the samples using OMNIC TM series software (ThermoFisher Scientific, Waltham, Massachusetts, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!