Anti cd34 ram34

Manufactured by Thermo Fisher Scientific

Anti-CD34 (RAM34) is a monoclonal antibody that recognizes the CD34 antigen, a surface glycoprotein expressed on hematopoietic stem and progenitor cells. This antibody is commonly used in various laboratory applications, including flow cytometry and immunohistochemistry, to identify and analyze CD34-positive cells.

Automatically generated - may contain errors

Lab products found in correlation

Facsaria, by BD (1 mentions) Histopaque 1077, by Merck Group (1 mentions) Anti cd117 microbeads, by Miltenyi Biotec (1 mentions) Fc block, by BD (1 mentions) Fixation permeabilization solution kit, by BD (1 mentions) Anti sca 1, by BioLegend (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by BioLegend (1 mentions) Accuri c6 flow cytometer, by BD (1 mentions) Pe conjugated anti ki 67 antibody, by BD (1 mentions) Anti phospho mtor ser2448, by Cell Signaling Technology (1 mentions) Anti phospho akt thr308, by Cell Signaling Technology (1 mentions) Anti cd45.1 a20, by BioLegend (1 mentions) Anti cd16 32 2.4g2, by BD (1 mentions) Anti b220 ra3 6b2, by BD (1 mentions) Anti cd4 rm4 5, by BD (1 mentions) Anti β actin, by Cell Signaling Technology (1 mentions) Anti ki67 b56, by BD (1 mentions) Anti c kit microbeads, by Miltenyi Biotec (1 mentions) Anti mac 1 m1 70, by BD (1 mentions) Anti ter119 ter 119, by BioLegend (1 mentions)

Spelling variants of this product

Anti-CD34 (23 citations) CD34 (RAM34) (8 citations) RAM34 (8 citations) Anti-CD34 (clone RAM34) (2 citations) Anti-CD34 coupled with eF660 (clone RAM34) (2 citations) FITC)-conjugated anti-CD34 (RAM34, (2 citations)

3 protocols using anti cd34 ram34

Isolation and Characterization of Murine Hematopoietic Stem and Progenitor Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bone marrow cells were harvested from 2, 12, and 24 month old C57Bl/6 male mice, and mononuclear cells were isolated using density gradient (Histopaque 1077, Sigma, St. Louis, MO). Mononuclear cells were then purified using anti-CD117 microbeads (Milteny Biotec, Aubum, CA). Cells in the CD117-positive fraction were stained with monoclonal antbodies and sorted on FACSAria (BD Bioscience, San Jose, CA). The following monoclonal antibodies were purchased from eBioscience (San Diego, CA) and used: anti-CD34 (RAM34) conjugated to FITC (fluorescein isothiocyanate); anti-FL2/Flt3 (A2F10) conjugated to phycoerythrin (PE); anti-CD127/Il7ra (A7R34) conjugated to PE-Cy5; anti-CD4 (GK1.5), -CD8 (53-6.7), -B220 (RA3-6B2), -Ter119 (TER119), -Mac-1 (M1/70), and anti-Gr-1 (RB6-8C5) conjugated to PE-Cy7; anti-c-Kit (2B8) conjugated to APC-Alexa Fluor 750. Monoclonal antibody anti-Sca-1 (E13-161-7) was purified from a hybridoma and conjugated to Pacific Blue in the Weissman laboratory.

Dimov I.K., Lu R., Lee E.P., Seita J., Sahoo D., Park S.M., Weissman I.L, & Lee L.P. (2014). Discriminating cellular heterogeneity using microwell-based RNA cytometry. Nature communications, 5, 3451.

+ Open protocol

+ Expand

Multiparameter Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cell suspension was preincubated with Fc block (BD Biosciences) to avoid nonspecific binding of antibodies. The following primary antibodies were used: anti-CD3 (145-2C11), anti-CD4 (RM4-5), anti-CD8 (53-6.7), anti-CD11b (M1/70), anti-B220 (RA3-6B2), anti-Gr-1 (RB6-8C5), anti-Ter119 (TER-119), anti-CD45 (30-F11), anti-FcRγII-III (93), anti-Sca-1 (E13-161.7), anti-c-Kit (2B8), anti-CD41 (MWReg30), anti-CD48 (HM-48-1), anti-CD150 (TC15-12F12.2), anti-IL7Rα (A7R34), anti-Flt3 (A2F10), anti-PDGFRα (APA5) (all from BioLegend, San Diego, CA) and anti-CD34 (RAM34; eBioscience, San Diego, CA). A mixture of CD4, CD8, CD11b, B220, Ter-119 and Gr-1 antibodies was used as the lineage (Lin) mixture. 7-AAD was used to identify and exclude dead cells. For cell cycle analysis, the cells were fixed and permeabilized using the Fixation/Permeabilization Solution kit (BD Biosciences) and were stained with PE-conjugated anti-Ki-67 antibody (BD Biosciences) and DAPI (BioLegend). The stained cells were analyzed and sorted using a FACSAria and an Accuri C6 flow cytometer (BD Biosciences), respectively. The flow cytometry data were analyzed using FlowJo ver. 10.0.5 (Treestar, Ashland, OH).

Igarashi H., Akahoshi N., Ohto-Nakanishi T., Yasuda D, & Ishii S. (2015). The lysophosphatidic acid receptor LPA4 regulates hematopoiesis-supporting activity of bone marrow stromal cells. Scientific Reports, 5, 11410.

+ Open protocol

+ Expand

Monoclonal Antibodies for Hematopoietic Cell Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following monoclonal antibodies (Abs) were used for flow cytometry and cell sorting: anti-c-Kit Microbeads (Miltenyi Biotec), anti-c-Kit (2B8, BD Biosciences / Biolegend), anti-Sca-1 (E13–161.7, BD Biosciences / Biolegend), anti-CD45.1 (A20, Biolegend), anti-CD45.2 (104, BD Biosciences), anti-CD4 (RM4–5, BD Biosciences), anti-B220 (RA3–6B2, BD Biosciences), anti-TER-119 (TER-119, Biolegend), anti-Gr-1 (RB6–8C5, BD Biosciences/Biolegend), anti-Mac-1 (M1/70, BD Biosciences), anti-CD71 (C2, BD Biosciences), anti-CD43 (S7, BD Biosciences), anti-CD16/32 (2.4G2, BD Biosciences), anti-Ki67 (B56, BD Biosciences), anti-CD34 (RAM34, eBioscience), anti-CD135 (A2F10, Biolegend), The primary Ab used for immunocytochemistry (ICC) was goat anti-Tfe3 (p-16; Santa Cruz Biotechnology). Anti-phospho mTor (Ser2448), anti-phospho Akt (Thr308), and anti-beta actin from Cell Signaling were used for western blotting.

Baba M., Toyama H., Sun L., Takubo K., Suh H.C., Hasumi H., Nakamura-Ishizu A., Hasumi Y., Klarmann K.D., Nakagata N., Schmidt L.S., Linehan W.M., Suda T, & Keller J.R. (2016). Loss of Folliculin disrupts hematopoietic stem cell quiescence and homeostasis resulting in bone marrow failure. Stem cells (Dayton, Ohio), 34(4), 1068-1082.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!