Shim pack gis 0.5 mm ods c18 column

Manufactured by Shimadzu

Sourced in Japan

The Shim-pack GIS 0.5 mm ODS C18 column is a high-performance liquid chromatography (HPLC) column that features a 0.5 mm internal diameter and a C18 stationary phase. The column is designed for efficient separation and analysis of a wide range of compounds.

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Lab products found in correlation

Spectromonitor 3200, by Shimadzu (1 mentions)

Close spelling variants of this product

Shim-pack GIS-ODS column GIS-ODS C18 column Shim-pack C18 column GIS-ODS column GIS C18 column ODS-C18 column GISS C18 C18 column

3 protocols using shim pack gis 0.5 mm ods c18 column

HPLC Analysis of Compounds

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For HPLC analysis, a Shimadzu SpectroMonitor 3200 digital UV-Vis detector equipped with a Shim-pack GIS 0.5 mm ODS C18 column (250 × 4.6 mm I.D.) was used (Shimadzu, Kyoto, Japan). The mobile phase consisted of water with 0.1% TFA (Solvent A) and acetonitrile (Solvent B). The gradient method was used with a flow rate of 1 mL/min and the analysis was done by increasing solvent B from 10% to 100% for 30 min.

Park T., Park J.S., Sim J.H, & Kim S.Y. (2020). 7-Acetoxycoumarin Inhibits LPS-Induced Inflammatory Cytokine Synthesis by IκBα Degradation and MAPK Activation in Macrophage Cells. Molecules, 25(14), 3124.

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HPLC Analysis of Compounds

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For HPLC analysis, a Shimadzu SpectroMonitor 3200 digital UV-Vis detector equipped with a Shim-pack GIS 0.5-mm ODS C18 column (250 × 4.6 mm id) was used (Shimadzu, Kyoto, Japan). The mobile phase consisted of water containing 0.1% v/v trifluoroacetic acid (TFA) (solvent A) and acetonitrile (solvent B). A gradient method was used with a flow rate of 1 mL/min of solvent B, which was increased from 10% to 100% over 30 min, maintained for 5 min, and then reduced to 10% over 3 min and maintained for 5 min.

Kim M.S., Park J.S., Chung Y.C., Jang S., Hyun C.G, & Kim S.Y. (2019). Anti-Inflammatory Effects of Formononetin 7-O-phosphate, a Novel Biorenovation Product, on LPS-Stimulated RAW 264.7 Macrophage Cells. Molecules, 24(21), 3910.

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HPLC Analysis of Organic Compounds

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A Shimadzu SpectroMonitor, 3200 digital UV/Vis detector, equipped with a Shim-pack GIS 0.5 mm ODS C18 column (250 mm × 4.6 mm I.D.) was used for HPLC analysis (Shimadzu, Kyoto, Japan). The mobile phase consisted of acetonitrile and water, with 0.1% Trifluoroacetic acid (TFA). A gradient method was used with a flow rate of 1 mL/min, and the analysis was performed by increasing solvent B (acetonitrile) from 10% to 100% for 30 min.

Sim J., Jang S., Park T., Chi W., & Kim S. (2020). Melanogenesis Effect of 7-acetoxy-4-methylcoumarin in B16F10 Melanoma Cells. Cosmetics, 7(4), 94-94.

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