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2 protocols using p her2 y1278

1

Immunoblotting and Chromatin Immunoprecipitation Assays

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This was performed as described previously.35 (link), 36 (link) Antibodies used in WB included mouse monoclonal antibodies against β-actin (Sigma), α-tubulin (Sigma), E-cadherin (610404, BD), p-HSF-1/S326 (ab76076, Epitomics/Abcam), p-JNK/S63/73 (sc-6254; Santa Cruz), as well as, rabbit antibodies against Slug (AP2053a,ABGENT), HSF-1 (4356, Cell Signaling), Hsp70 (4876, Cell Signaling), p38 (9212, Cell Signaling), p-p38/T180/Y182 (4511, Cell Signaling), pERK/T202/Y204 (9109, Cell Signaling), Akt/pan (4691, Cell Signaling), p-Akt/S473 (4060, Cell Signaling), mTOR (2983, Cell Signaling), p-mTOR/S2448 (5536, Cell Signaling), JNK (sc-571, Santa Cruz), HER2 (2165, Cell Signaling), p-HER2/Y1278 (2247, Cell Signaling). Rabbit polyclonal HSF-1 antibody used in IP and ChIP assay was from Cell signaling (4356).
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2

Immunoblotting and Chromatin Immunoprecipitation Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
This was performed as described previously.35 (link), 36 (link) Antibodies used in WB included mouse monoclonal antibodies against β-actin (Sigma), α-tubulin (Sigma), E-cadherin (610404, BD), p-HSF-1/S326 (ab76076, Epitomics/Abcam), p-JNK/S63/73 (sc-6254; Santa Cruz), as well as, rabbit antibodies against Slug (AP2053a,ABGENT), HSF-1 (4356, Cell Signaling), Hsp70 (4876, Cell Signaling), p38 (9212, Cell Signaling), p-p38/T180/Y182 (4511, Cell Signaling), pERK/T202/Y204 (9109, Cell Signaling), Akt/pan (4691, Cell Signaling), p-Akt/S473 (4060, Cell Signaling), mTOR (2983, Cell Signaling), p-mTOR/S2448 (5536, Cell Signaling), JNK (sc-571, Santa Cruz), HER2 (2165, Cell Signaling), p-HER2/Y1278 (2247, Cell Signaling). Rabbit polyclonal HSF-1 antibody used in IP and ChIP assay was from Cell signaling (4356).
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