Sc 29679

Manufactured by Santa Cruz Biotechnology

Sourced in United States

sc-29679 is a lab equipment product offered by Santa Cruz Biotechnology. The core function of this product is to facilitate scientific research and experiments. No further details are available while maintaining an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

4d nucleofector, by Lonza (1 mentions) Sc 43390, by Santa Cruz Biotechnology (1 mentions) Sc 72578, by Santa Cruz Biotechnology (1 mentions) Sc 7020, by Santa Cruz Biotechnology (1 mentions) Sc 44230, by Santa Cruz Biotechnology (1 mentions) Mk 2206 2hcl s1078, by Selleck Chemicals (1 mentions)

2 protocols using sc 29679

Silencing p62 and LC3B in THP-1 Cells

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THP-1 cells were electroporated with small interfering RNA (siRNA) against human p62 (sc-29679), LC3B (sc-43390), or corresponding control siRNA (sc-37007) (all from Santa Cruz Biotechnology, Santa Cruz, CA, USA), using SF Cell Line 4D-Nucleofector X Solution and program DV-100 on a 4D-Nucleofector (Lonza, Basel, Switzerland) following the manufacturer’s instructions. After transfection, cells were transferred to 12-well plates (1 × 10⁶ cells/well) and incubated in a complete cell culture medium with 5% FBS, as described in the figure legends.

Paunovic V., Vucicevic L., Misirkic Marjanovic M., Perovic V., Ristic B., Bosnjak M., Mandic M., Stevanovic D., Harhaji-Trajkovic L., Lalosevic J., Nikolic M., Bonaci-Nikolic B, & Trajkovic V. (2023). Autophagy Receptor p62 Regulates SARS-CoV-2-Induced Inflammation in COVID-19. Cells, 12(9), 1282.

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Autophagy Regulation in Host-Pathogen Interactions

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Rapamycin (Rapa, R8781), 3-methyladenine (3-MA, M9281), bafilomycin A1 (Baf-A1, B1793), antibodies against ATG12 (WH0009140M1), MAP1LC3B (L7543), and ATG5 (WH0009474M1) were purchased from Sigma-Aldrich (Shanghai, CHINA), and MK-2206 2HCL (S1078) purchased from Selleckchem (Houston, TX, USA). Antibodies against Akt (9272), mTOR (2972), AMPK (2532), phospho-Akt (Ser473) (4060), LAMP1 (9091), phospho-mTOR (Ser2448) (5536), phospho-S6 ribosomal protein (Ser235/236) (2211), ribosomal protein (2217), phospho-c-Met (Y1234/Y1235) (4033), and c-Met (4560) were obtained from Cell Signaling Technology (Beverly, MA, USA), whereas antibodies against β-actin (sc-10731), VacA (sc-25790), CagA (sc-17450), phospho-tyrosine (PY99) (sc-7020) and SQSTM1 (sc-28359), and siRNAs specific for SQSTM1 (human, sc-29679), ATG12 (human, sc-72578), c-Met (human, sc-29397), and ATG5 (human, sc-41445), along with a control siRNA (sc-44230) were obtained from Santa Cruz Biotechnology (CA, USA).

Li N., Tang B., Jia Y.P., Zhu P., Zhuang Y., Fang Y., Li Q., Wang K., Zhang W.J., Guo G., Wang T.J., Feng Y.J., Qiao B., Mao X.H, & Zou Q.M. (2017). Helicobacter pylori CagA Protein Negatively Regulates Autophagy and Promotes Inflammatory Response via c-Met-PI3K/Akt-mTOR Signaling Pathway. Frontiers in Cellular and Infection Microbiology, 7, 417.

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