Cells were seeded at 5000 cells per well in round bottom clear 96 well plates (USA, Costar, Fisher Scientific—cat 7007) suspended in RPMI 10% FBS, 1% PS containing 1:10 dilution of spheroid formation buffer (Thermofisher—cat 3500-096-K, USA). The plate was then centrifuged 200× g at 20 °C for 3 min before incubation at 37 °C for 3 days. Gel matrix (Thermofisher—cat 3500-096-K, USA) was then added to each well and the plate was centrifuged 300× g at 4 °C for 5 min before incubation at 37 °C for 1 h. Variables were then added in RPMI (1%FBS, 1% PS) to each well [Vehicle (PBS1x) or, zoledronate 1, 3, 10 µM] and the plate was re-incubated at 37 °C for 1, 4, 7 and 12 days. Images were captured using a Canon powershot camera A640 with a Soligor adaptor 426,126 and an inverted light microscope Axiovert 40 C (Zeiss, Thornwood, NY, USA) at 10× magnification. Area of spheroid invasion was measured using the ImageJ software.
Powershot a640 camera
Manufactured by Canon
Sourced in Japan, United States, Canada
The PowerShot A640 is a digital camera designed for general photography. It features a 10.0-megapixel image sensor and a 4x optical zoom lens. The camera supports various shooting modes and image file formats.
Automatically generated - may contain errors
Lab products found in correlation
Pageruler unstained protein ladder, by Thermo Fisher Scientific (2 mentions)
Mini protean tetra gel cell, by Bio-Rad (2 mentions)
Rapidstain, by G Biosciences (2 mentions)
Axiovert 40 c inverted light microscope, by Canon (2 mentions)
52 mm soligor adaptor tube, by Canon (2 mentions)
Axiovert 40c, by Zeiss (1 mentions)
Axiolab, by Canon (1 mentions)
Rotor hda, by Singer Instruments (1 mentions)
Carboxymethylcellulose, by Merck Group (1 mentions)
Crystal violet, by Merck Group (1 mentions)
Axiovert 40 cfl inverted microscope, by Canon (1 mentions)
Axiovision software, by Zeiss (1 mentions)
Axioskop 40 microscope, by Zeiss (1 mentions)
Lsm 510 meta confocal microscope, by Zeiss (1 mentions)
Bx53 fluorescence microscope, by Olympus (1 mentions)
Dfc425 camera, by Leica (1 mentions)
Dp80 digital camera, by Olympus (1 mentions)
Szx7 microscope, by Canon (1 mentions)
Matrigel basement membrane matrix, by BD (1 mentions)
Eclipse ti e microscope, by Nikon (1 mentions)
17 protocols using powershot a640 camera
1
Spheroid Formation and Zoledronate Assay
2
Histopathological Analysis of Liver and Kidney
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The liver and kidney tissues were fixed in 10% formalin for 48 h, then dehydrated in graduated ethanol (50-100%) and embedded in paraffin. Sections 5µm thick were made and stained with hematoxylin and eosin dye, then examined for histopathological changes under light microscopy (ZEISS, Axiolab) and fitted with Canon Power Shot camera (A640) to capture images for histological studies. All sections were evaluated to know about for the degree of liver and kidney injury.
3
SDS-PAGE Protein Separation Protocol
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20 μg of protein samples were mixed with 2× loading buffer [38 (link)] and heated to 95°C for 6 min. A protein ladder (PageRuler unstained protein ladder, Thermo Fischer Scientific Inc., Waltham, MA, USA) and the protein sample were loaded on a SDS mini gel containing 12% acrylamide resolving gel and 5% stacking gel prepared according to [39 ]. The gel was separated in a Mini-Protean® Tetra gel cell (Bio-Rad, Herculas, CA, USA) by applying 80 V for 30min followed by 180 V for 65min. A fluorescent picture was taken at 312nm irradiation using a UV transilluminator (UV star, Bio-Rad), a PowerShot A640 camera (Canon, Tokyo, Japan) and a 520 nm long pass filter. The gel was stained with RAPIDstain™ (G-Biosciences, St. Louis, MO, USA).
4
Neurite Outgrowth Microscopy Analysis
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Each medium was applied in duplicate wells and two random images per well were taken using a Zeiss Axiovert 40 C inverted light microscope (Toronto, ON, Canada) with a Canon PowerShot A640 camera and 52 mm Soligor adaptor tube (Mississauga, ON, Canada). The percentage of cells with neurites was determined and then averaged for each experimental condition.
5
Surface Characterization of CSH Films
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The surface of the CSH films was observed with a Nikon Eclipse E100 light microscope (Nikon Corporation, Kanagawa, Japan) and analyzed at least five times (at six-day intervals) during the soil burial test. After washing the samples with distilled water, images of dried CSH film samples (×10 and ×40 magnification) were taken using a Canon PowerShot A640 camera (Melville, NY, USA).
6
Systematic Genetic Interaction Mapping
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SGA analysis was performed as described in [72 ] in two replicates. The query strain (KAY27) was prepared by introducing the mutation prp45(1–169) in the strain Y9072. Y8835 served as a control strain expressing the nourseothricin N-acetyl transferase cassette. The query and control strains were crossed with the deletion mutant array [73 (link)]. All manipulations were performed robotically using RoToR HDA (Singer Instruments) in 1536-dot format (four replicate spots per strain). The final plates were incubated at 30° and 35°C and the growth of the double mutant progeny was documented after one, two and three days of growth using a Canon PowerShot A640 camera. Colony sizes were analysed using computer scoring software (http://sgatools.ccbr.utoronto.ca/ ). Hits were confirmed by random spore analysis, measurements of growth rates in liquid culture, or by tetrad dissection after crossing the strains de novo. Except for hits where the colonies were consistently much smaller at all SGA plates across all temperatures, all other SGA hits were validated. Those that remained dubious were excluded or the double mutants were prepared de novo (see STab. S4 for details). Primary SGA data, list of hits containing their computed scores and verification status, and the results of the Gene Ontology (GO) term analysis are available in STab. S3, S4, and S5, respectively.
7
Catalase Activity Profiling by SDS-PAGE
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Aliquots (10 µL) of each pre-incubated catalase (9 µL of untreated catalase and 1 µL of deionized water) were mixed with 10 µL of 2× loading buffer [44 (link)] and heated to 95 °C for 6 min. A protein ladder (PageRuler unstained protein ladder, Thermo Scientific) and 15 µL of each sample were loaded on a 12% SDS mini gel and separated in a Mini-Protean® Tetra gel cell (Bio-Rad, Herculas, CA, USA) by applying 80 V for 30 min followed by 180 V for 65 min. A fluorescent picture was taken at 365 nm irradiation using a UV transilluminator (Bio-Rad, UV star), a PowerShot A640 camera (Canon, Tokyo, Japan) and a commercially available UV filter (HMC Hoya Multi-Coated Filter, Hoya, Tokyo, Japan). The gel was stained with RAPIDstain™ (G-Biosciences, St. Louis, MO, USA).
8
VACV Plaque Assay in MEFs
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Semiconfluent monolayers of TRAF2+/+ and TRAF2−/− MEFs were infected with VACV for 1 h at 37°C. The inoculum was removed (time point 0 h), cells were washed with medium, 2 ml of 1.5% carboxy methylcellulose (Sigma) in 2.5% DMEM was added, and cells were incubated for 2 days at 37°C. The semisolid overlay was then aspirated, and cells were washed briefly with PBS and stained with 0.1% (wt/vol) crystal violet (Sigma) in 15% ethanol. Images of individual plaques were taken using a Zeiss Axiovert 40 CFL inverted microscope and a Canon Powershot A640 camera. The diameter of each plaque was measured using Adobe Photoshop image software.
9
Bioluminescent Imaging of Nanoparticle Localization
10
Fluorescence Microscopy Imaging Protocol
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Cells were observed under phase contrast and epifluorescence under a Zeiss Axioskop 40 microscope (Carl Zeiss, Göttingen, Germany) with 40 X and 63 X objectives under the FITC excitation/emission filter. Data were acquired using a Canon PowerShot A640 camera (Canon Inc., Japan) and the Carl-Zeiss AxioVision software (Carl-Zeiss). Alternatively, CLSM images were acquired using a Zeiss LSM 510 META confocal microscope (Carl Zeiss) equipped with an argon and a helium/neon laser. GFP fluorescence was imaged using excitation with the 488-nm line of the argon laser.
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