Ccrf cem

Immortalized T lymphocyte cell line (Jurkat), a promyelocytic cell line derived from human leukemia (HL60), a human myelomonocytic tumor cell line (U937), and a human T lymphoblasts tumor cell line (CCRF-CEM) was cultured in RPMI-1640-medium, and two human hepatocellular carcinomas cell lines (HepG2 and SMCC7721) were cultured in DMEM medium, with 10% fetal bovine serum (FBS) in humidified air at 37 °C with 5% CO₂. Then these tumor cells were seeded into 96-well plates at 6–10 × 10³ cells/well, treated with the complex 1–3 at different concentrations after these adherent cells hatched for 12 h at 37 °C with 5% CO₂. (The final concentrations of these compounds were 25, 10, 5, 2.5, 1, 0.1, and 0.01 μM). After 72 h treatment, the cells were incubated with 15 μL MTT solution (5 mg/mL) for 4 h at 37 °C with 5% CO₂. The formazan precipitates were dissolved in 100 mL DMSO. At 490 nm, the absorbance was measured by Infinite M1000 PRO (TECAN). The Jurkat, U937, CCRF-CEM, HL-60, and HepG2 cell lines were purchased from the National Collection of Authenticated Cell Cultures (Shanghai, China). The SMCC7721 cells were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

ALL cell lines, including Jurkat, CCRF-CEM, CEM/C1, Nalm6, Sup-B15, THP-1, HL-60, and Reh cells, and the human embryonic kidney cell line 293T, were purchased from the National Collection of Authenticated Cell Cultures (Shanghai, China). CCRF-CEM, CEM/C1, Nalm6, and Reh cells were cultured in RPMI 1640 medium (Gibco, Carlsbad, CA) containing 10% fetal bovine serum (FBS, Corning, New Zealand Sourced), and 1% penicillin–streptomycin (Gibco). Jurkat cells were cultured with RPMI 1640 containing 10% FBS, 1% GlutaMAX (Gibco), and 1% penicillin–streptomycin. THP-1 cells were cultured in RPMI 1640 containing 10% FBS, 0.05 mmol/l β-Mercaptoethanol (Gibco), and 1% penicillin–streptomycin. HL-60 and Sup-B15 cells were cultured in IMDM (Gibco) containing 20% FBS and 1% penicillin–streptomycin. 293T cells were cultured with Dulbecco’s modified Eagle’s medium (DMEM) (Gibco) containing 10% FBS and 1% penicillin–streptomycin. All cells were maintained at 37°C with 5% CO₂, and saturated humidity. Cells in the logarithmic growth phase were used for subsequent experiments. All cells were identified by short tandem repeat (STR) profiling and were mycoplasma negative.