S2302 h d prolyl l phenylalanyl l arginine p nitroanilide

Manufactured by Diapharma

S2302 (H-D-prolyl-L-phenylalanyl-L-arginine-p-nitroanilide) is a synthetic peptide substrate for the measurement of the enzyme activity of thrombin. It is commonly used in laboratory settings to assess thrombin-like enzymatic activity.

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Lab products found in correlation

S 2366 l pyro glu l pro l arg p nitroanilide, by Diapharma (3 mentions) Corn trypsin inhibitor, by Enzyme Research (3 mentions) Kaolin, by Thermo Fisher Scientific (2 mentions) Hrp conjugated antihemagglutinin ha igg, by Thermo Fisher Scientific (2 mentions) Soybean trypsin inhibitor sbti, by Merck Group (2 mentions) Activated partial thromboplastin time reagent, by Diagnostica Stago (2 mentions) Streptavidin hrp, by Thermo Fisher Scientific (2 mentions) Horseradish peroxidase conjugated hrp goat antihuman fxii, by Enzyme Research (2 mentions) Dextran sulfate, by Merck Group (1 mentions) Ellagic acid, by Thermo Fisher Scientific (1 mentions) Argatroban, by GlaxoSmithKline (1 mentions) ε aminocaproic acid εaca, by Merck Group (1 mentions) Ptt a reagent, by Diagnostica Stago (1 mentions) Soybean trypsin inhibitor, by Merck Group (1 mentions) Polyethyleneimine, by Merck Group (1 mentions) α thrombin, by Enzyme Research (1 mentions) βfxiia, by Enzyme Research (1 mentions)

3 protocols using s2302 h d prolyl l phenylalanyl l arginine p nitroanilide

Quantification of FXII and PK Activation

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Materials used were as follows: normal human plasma (Precision BioLogic, Dartmouth, Canada); FXII-deficient plasma (George King Biomed); FXII, FXIIa, βFXIIa, PK, PKa, α-thrombin, corn trypsin inhibitor, and horseradish peroxidase–conjugated goat anti-human FXII and anti-human PK (Enzyme Research Laboratory, South Bend, IN); dextran sulfate (500 000 Da), polyethyleneimine (PEI; 750 000 Da), soybean trypsin inhibitor, and ε-aminocaproic acid (ε-ACA; Sigma-Aldrich, St. Louis, MO); ellagic acid (ACROS Organics, The Hague, Netherlands); S2302 (H-d-prolyl-l-phenylalanyl-l-arginine-p-nitroanilide) and S-2366 (l-pyro-Glu-l-Pro-l-Arg-p-nitroanilide; DiaPharma, Westchester, OH); PTT-A reagent (Diagnostica Stago, Asnieres-sur-Seine, France); argatroban (GlaxoSmithKline, Brentford, United Kingdom); polyphosphate (poly-P; 200-1300 units; provided by James Morrissey, University of Michigan); and horseradish peroxidase–conjugated antihemagglutinin immunoglobulin G (IgG; Invitrogen). Monoclonal IgGs 15H8 and 1B2^{8,18 (link)} are described.

Shamanaev A., Ivanov I., Sun M.F., Litvak M., Srivastava P., Mohammed B.M., Shaban R., Maddur A., Verhamme I.M., McCarty O.J., Law R.H, & Gailani D. (2022). Model for surface-dependent factor XII activation: the roles of factor XII heavy chain domains. Blood Advances, 6(10), 3142-3154.

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Assay of Contact Activation Factors

Cited 1 time

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Human pooled normal plasma (Precision BioLogic). FXII-deficient plasma (George King Biomed). FXII, FXIIa, βFXIIa, PK, PKa, XI, XIa, corn-trypsin inhibitor (CTI), and horseradish peroxidase-conjugated (HRP) goat antihuman FXII (Enzyme Research Laboratory). Soybean trypsin inhibitor (SBTI) (Sigma-Aldrich). S2302 (H-D-prolyl-L-phenylalanyl-L-arginine-p-nitroanilide) and S-2366 (L-pyro-Glu-L-Pro-L-Arg-p-nitroanilide (DiaPharma). Activated partial thromboplastin time (aPTT) reagent (Diagnostica Stago). Kaolin (ACROS Organics). Phe-Pro-Arg-chloromethylketone (FPRCK) (Prolytix). Long-chain PolyP with a broad range of polymer lengths (200-1300 orthophosphate units), and size-fractionated short-chain PolyP (PolyP-31, 27-35 units) have been described [12 (link)]. HRP-conjugated antihemagglutinin (HA) IgG (Invitrogen). Monoclonal biotinylated anti-FXI IgG 14E11 has been described [13 (link)]. Streptavidin-HRP (Thermo Scientific).

Hara-Kudo Y., Ikedo M., Kodaka H., Nakagawa H., Goto K., Masuda T., Konuma H., Kojima T, & Kumagai S. (2000). Selective Enrichment with a Resuscitation Step for Isolation of Freeze-Injured Escherichia coli O157:H7 from Foods. Applied and Environmental Microbiology, 66(7), 2866-2872.

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Intrinsic Coagulation Pathway Analysis

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Human pooled normal plasma (Precision BioLogic). FXII-deficient plasma (George King Biomed). FXII, FXIIa, βFXIIa, PK, PKa, XI, XIa, corn-trypsin inhibitor (CTI), and horseradish peroxidase-conjugated (HRP) goat antihuman FXII (Enzyme Research Laboratory). Soybean trypsin inhibitor (SBTI) (Sigma-Aldrich). S2302 (H-D-prolyl-L-phenylalanyl-L-arginine-p-nitroanilide) and S-2366 (L-pyro-Glu-L-Pro-L-Arg-p-nitroanilide (DiaPharma). Activated partial thromboplastin time (aPTT) reagent (Diagnostica Stago). Kaolin (ACROS Organics). Phe-Pro-Arg-chloromethylketone (FPRCK) (Prolytix). Long-chain PolyP with a broad range of polymer lengths (200-1300 orthophosphate units), and size-fractionated short-chain PolyP (PolyP-31, 27-35 units) have been described [12] . HRP-conjugated antihemagglutinin (HA) IgG (Invitrogen). Monoclonal biotinylated anti-FXI IgG 14E11 has been described [13] . Streptavidin-HRP (Thermo Scientific).

Shamanaev A., Litvak M., Cheng Q., Ponczek M., Dickeson S.K., Smith S.A., Morrissey J.H, & Gailani D. (2023). A site on factor XII required for productive interactions with polyphosphate. Journal of thrombosis and haemostasis : JTH, 21(6).

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