Apollo 2 electrospray ion source

Exudate samples as well as water controls were analysed by ultra performance liquid chromatography coupled to electron spray ionisation quadrupole time of flight mass spectrometry (UPLC/ESI-Q-ToF-MS). An ultra performance liquid chromatography (ACQUITY UPLC; Waters, Eschborn, Germany) equipped with an ACQUITY UPLC® HSS T3 column (ACQUITY UPLC HSS T3 Column, 100 Å, 1.8 µm, 1 mm × 100 mm; Waters) coupled to MicrOTOF–Q II hybrid quadrupole time-of-flight mass spectrometer equipped with an Apollo II electrospray ion source (Bruker Daltonics) was used for MS mode. To obtain CID mass spectra (MS/MS) of exuded compounds UPLC/ESI-Q-ToF-MS with an ultra performance ACQUITY UPLC platform (ACQUITY UPLC; Waters) equipped with an Aquity UPLC® H5S T3 column (ACQUITY UPLC HSS T3 Column, 100 Å, 1.8 µm, 3 mm × 100 mm, 1/pkg; Waters) and a MicrOTOF–Q I hybrid quadrupole time-of-flight mass spectrometer equipped with an Apollo II electrospray ion source (Bruker Daltonics). Detailed description were provided in the publication of Dietz et al.²¹ .

For LC/MS-based metabolite profiling (UPLC: Acquity, Waters, Eschborn, Germany; MS: MicrOTOF–Q I hybrid quadrupole time-of-flight mass spectrometer equipped with an Apollo II electrospray ion source, Bruker Daltonik GmbH, Bremen, Germany), the ground tissue material was processed by solid liquid extraction using methanol/water, 80/20 (v/v) (40 mg root fresh weight corresponds to 200 µL extraction solution and 50 mg leaf fresh weight corresponds to 400 µL extraction solution). Analytes of the nutrient solution were extracted by a reversed-phase solid phase extraction procedure (180 mL medium result in 120 µL analysis solution).

Mycelium extracts (20-fold concentrated) were measured on an Acquity UPLC system (Waters, www.waters.com) coupled to a MicrOTOF–Q I hybrid quadrupole time-of-flight mass spectrometer. For this purpose, samples were ionized at positive ESI(+) and negative ESI(−) ionization using an Apollo II electrospray ion source (Bruker Daltonics, www.bruker.com). All mass spectra were acquired in centroid mode and recalibrated on the basis of lithium formate cluster ions. DataAnalysis 4.2 (Bruker Daltonics) was used for the generation of extracted ion chromatograms, deconvolution of mass spectra and calculation of elemental compositions. γ-Glu-Leu was quantified using Quant Analysis 4.2.