Log In Sign up
The largest database of trusted experimental protocols

410 mt cf

Manufactured by R&D Systems
Visit Supplier

The 410-MT/CF is a piece of laboratory equipment. It is designed for the separation and concentration of cells or other particles from liquid samples. The core function of this product is to facilitate the isolation and purification of target analytes from complex matrices.

Automatically generated - may contain errors

Lab products found in correlation

L929 cell, by American Type Culture Collection (1 mentions) 7666 mb cf, by R&D Systems (1 mentions) 404 ml cf, by R&D Systems (1 mentions) 401 ml cf, by R&D Systems (1 mentions) A92902, by Merck Group (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Huvecs, by Thermo Fisher Scientific (1 mentions) Cc 2159, by Lonza (1 mentions) Huvecs, by R&D Systems (1 mentions) 210 ta cf, by R&D Systems (1 mentions) Precisionglide needle, by BD (1 mentions) Af 410 na, by R&D Systems (1 mentions) Microliter syringe, by Hamilton Company (1 mentions)

Spelling variants of this product

410-MT-025/CF (3 citations) 410-MT-010/CF (2 citations)

4 protocols using 410 mt cf

1

Collagen Deposition in BMDM-Fibroblast Co-culture

Check if the same lab product or an alternative is used in the 5 most similar protocols
GFPtpz-collagen BMDMs were seeded on matrigel-coated coverslips at a cell density of 3 × 104 cells/cm2 together with murine L929 cells (1.5 × 104 cells/cm2, obtained from the American Type Culture Collection). In order to stimulate collagen deposition, growing medium (DMEM with 10% FBS, 2 mM L-glutamine and 2% penicillin/streptomycin) was supplemented with a combination of supernatants (SNTs) from stimulated L929 fibroblasts and Mouse primary Cardiac microvascular Endothelial Cells, MCEC, (C57-6024, obtained from Cell Biologics) in a ratio of 2:1:1 (growing medium:L929 SNT:MCEC SNT) during 6 to 9 days. To facilitate collagen deposition, 50 µg/ml of fresh ascorbic acid (A92902, Sigma-Aldrich) was added daily and medium supplemented with SNTs was changed every 2 days. For supernatants preparation, L929 and MCEC monoculture were grown to confluence in DMEM supplemented with 10% FBS, 2 mM L-glutamine and 2% penicillin/streptomycin. L929 cells medium was supplemented with 10 ng/ml TNFα (410-MT/CF, R&D Systems), 10 ng/ml IL-1β (401-ML/CF, R&D Systems), 10 ng/ml IL-4 (404-ML/CF, R&D Systems) and 10 ng/ml TGFβ (7666-MB/CF, R&D Systems). MCEC medium was supplemented with 10 ng/ml TNFα (410-MT/CF, R&D Systems). After 48 h of incubation, the supernatants from both monocultures were harvested, centrifuged to remove cell debris and stored at −20 °C until use.
Simões F.C., Cahill T.J., Kenyon A., Gavriouchkina D., Vieira J.M., Sun X., Pezzolla D., Ravaud C., Masmanian E., Weinberger M., Mayes S., Lemieux M.E., Barnette D.N., Gunadasa-Rohling M., Williams R.M., Greaves D.R., Trinh L.A., Fraser S.E., Dallas S.L., Choudhury R.P., Sauka-Spengler T, & Riley P.R. (2020). Macrophages directly contribute collagen to scar formation during zebrafish heart regeneration and mouse heart repair. Nature Communications, 11, 600.
+ Open protocol
+ Expand
2

Isolation and Culture of Endothelial Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
HUVECs were purchased from Lonza (cc-2159) and cultured in EC growth medium EGM-2 (cc-3162). Cells were plated on 12-well plates at 60,000/well and allowed to grow to 80%–90% confluency. In some experiments, HUVECs were stimulated with 10 ng/ml of recombinant human TNF-α (210-TA/CF, R&D Systems) for 4 h. After stimulation, HUVECs were harvested for RNA isolation using TRIzol reagent (15596018, Invitrogen). Cells were passaged less than five times for all experiments. Primary lung and liver ECs from miR-181a2b2flox/flox, miR-181a2b2 knock out (miR-181a2b2−/−), or ECs-specific miR-181a2b2 deficient mice (miR-181a2b2iECKO) mice were isolated and cultured as described in our previous works [10 (link),12 ]. Lung or liver ECs were stimulated with or without 20 ng/ml recombinant mouse TNF-α (410-MT/CF, R&D Systems) for 4 or 8 h and harvested for cell adhesion assay or Western blot.
Yang D., Haemmig S., Chen J., McCoy M., Cheng H.S., Zhou H., Pérez-Cremades D., Cheng X., Sun X., Haneo-Mejia J., Vellarikkal S.K., Gupta R.M., Barrera V, & Feinberg M.W. (2022). Endothelial cell-specific deletion of a microRNA accelerates atherosclerosis. Atherosclerosis, 350, 9-18.
+ Open protocol
+ Expand
3

TNF-α Depletion and Combination Therapy

Check if the same lab product or an alternative is used in the 5 most similar protocols
Procedures similar to those described for the anti-IL-1β antibody or recombinant IL-1β were used. To deplete TNF-α, mice were injected with 50 μg of a TNF-α-specific antibody (AF410-NA; R&D Systems) 1 day before infection by bacteria and then twice per week for 2 weeks thereafter. The Salmonellae and TNF-α combination therapy groups received an intratumoral injection of recombinant TNF-α (410-MT/CF; 0.25 μg in PBS; R&D Systems) every 2 days starting at 5 dpi and continuing until 11 dpi. Recombinant TNF-α injections were performed using a Microliter syringe (Hamilton Company) fitted with a PrecisionGlide Needle (BDM011455-1; BD Biosciences).
Kim J.E., Phan T.X., Nguyen V.H., Dinh-Vu H.V., Zheng J.H., Yun M., Park S.G., Hong Y., Choy H.E., Szardenings M., Hwang W., Park J.A., Park S., Im S.H, & Min J.J. (2015). Salmonella typhimurium Suppresses Tumor Growth via the Pro-Inflammatory Cytokine Interleukin-1β. Theranostics, 5(12), 1328-1342.
+ Open protocol
+ Expand
4

Hypothermia Induction by Murine TNF

Check if the same lab product or an alternative is used in the 5 most similar protocols
Seven-week old C57BL/6 J wild-type female mice received 1% hydroxyethylcellulose (Sigma 09368) by oral gavage followed 1 h later by 300 µg/kg recombinant murine TNF (R&D Systems 410-MT-CF) intravenously via tail vein. TNF was dissolved and diluted in endotoxin-free PBS (EMD Millipore TMS-12-A). Core body temperature was recorded by digital rectal probe as baseline before injection and every hour thereafter. Mice with a core body temperature below 30.0 °C were euthanized.
Bader S.M., Preston S.P., Saliba K., Lipszyc A., Grant Z.L., Mackiewicz L., Baldi A., Hempel A., Clark M.P., Peiris T., Clow W., Bjelic J., Stutz M.D., Arandjelovic P., Teale J., Du F., Coultas L., Murphy J.M., Allison C.C., Pellegrini M, & Samson A.L. (2022). Endothelial Caspase-8 prevents fatal necroptotic hemorrhage caused by commensal bacteria. Cell Death and Differentiation, 30(1), 27-36.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!