Mouse cells were lysed as described previously6 (link). Protein extracts were fractionated on 8% (for LMP1 immunoblot), 10% (for Trp1 immunoblot) or 15% (for Survivin and EphA2 immunoblots) sodium dodecyl sulfate polyacrylamide gels, transferred onto polyvinylidene difluoride membranes and immunoblotted with the following primary antibodies: anti-LMP1 (clone S12, 1:4; obtained as described previously9 (link)), anti-Survivin (clone 71G4B7, 1:1000; Cell Signaling), anti-EphA2 (clone C-3, 1:200; Santa Cruz), anti-Trp1 (clone TA99, 1:100; Santa Cruz), anti-Flag (clone M2, 1:5000; Sigma) and anti-GAPDH (clone 2118, 1:1000; Cell Signaling). Images were taken with ImageQuant LAS 4000 using ImageQuant LAS 4000 v1.2 software (both GE Healthcare Life Sciences).
Anti survivin clone 71g4b7
Manufactured by Cell Signaling Technology
Anti-Survivin (clone 71G4B7) is a mouse monoclonal antibody that recognizes the apoptosis inhibitor protein Survivin. This antibody is intended for research use only and its core function is to detect and quantify Survivin expression in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti flag clone m2, by Merck Group (2 mentions)
Anti lmp1 clone s12, by Cell Signaling Technology (2 mentions)
Anti epha2 clone c 3, by Santa Cruz Biotechnology (2 mentions)
Anti trp1 clone ta99, by Santa Cruz Biotechnology (2 mentions)
Anti gapdh clone 2118, by Cell Signaling Technology (2 mentions)
Imagequant las 4000 v1, by GE Healthcare (2 mentions)
Imagequant las 4000, by GE Healthcare (2 mentions)
2 protocols using anti survivin clone 71g4b7
1
Immunoblotting Analysis of Protein Extracts
2
Immunoblotting Analysis of Protein Extracts
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Mouse cells were lysed as described previously6 (link). Protein extracts were fractionated on 8% (for LMP1 immunoblot), 10% (for Trp1 immunoblot) or 15% (for Survivin and EphA2 immunoblots) sodium dodecyl sulfate polyacrylamide gels, transferred onto polyvinylidene difluoride membranes and immunoblotted with the following primary antibodies: anti-LMP1 (clone S12, 1:4; obtained as described previously9 (link)), anti-Survivin (clone 71G4B7, 1:1000; Cell Signaling), anti-EphA2 (clone C-3, 1:200; Santa Cruz), anti-Trp1 (clone TA99, 1:100; Santa Cruz), anti-Flag (clone M2, 1:5000; Sigma) and anti-GAPDH (clone 2118, 1:1000; Cell Signaling). Images were taken with ImageQuant LAS 4000 using ImageQuant LAS 4000 v1.2 software (both GE Healthcare Life Sciences).
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