Bt 549

MCF10A cells were cultured as previously described^{45 (link)}, HO15.19 (kindly provided by Dr. John Sedivy), MDA-MB-231 (HTB-26), HS 578T (HTB-126), HCT116 (CCL-247), and HeLa (CCL-2) cells were cultured in DMEM supplemented with 10% FBS, HCC1937 (CRL-2336), BT549 (HTB-122), HCC1954 (CRL-2338), A549 (CRM-CCL-185), HCC827 (CRL-2868), Raji (CCL-86, kindly provided by Dr. Eleanor Fish), H520 (HTB-182, kindly provided by Dr. Christine Allen), H2170 (CRL-5928), and Daudi (CCL-213) cells were cultured in RPMI-1640 supplemented with 10% FBS, OCI-AML2 (ACC-99) and OCI-AML3 (ACC-582) cells were cultured in Alpha-MEM supplemented with 10% FBS, HEC-1-A cells (HTB-112) were cultured in McCoy’s 5A supplemented with 10% FBS, and HMEC (CRL-3243) cells were cultured in MEGM with BPE, hEGF, hydrocortisone, GA-1000, and insulin (Lonza CC-3150). HCT116 FBXW7−/− or +/+ cells were obtained from Dr. Lars-Gunner Larson with permission from Dr. Bert Vogelstein.

The human breast cancer cell lines MCF7, T47D, MDA-MB-361, HCC1954, MDA-MB-468, MDA-MB-231, and BT-549 were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). MCF7, MDA-MB-361 and MDA-MB-231 cells were routinely cultured in Dulbecco's modified Eagle's medium (DMEM, Lonza, Walkersville, MD, USA), and T-47D, HCC1954, MDA-MB-468 and BT-549 were maintained in RPMI-1640 medium (Lonza), all supplemented with 10% fetal bovine serum (FBS, Sigma-Aldrich Co. LLC. St. Louis, MO, USA), 100 units/ml penicillin, and 100 mg/ml streptomycin. All cells were cultured at 37°C in a humidified atmosphere of 5% CO₂.

TNBC lines MDA-MB231 (ATCC HTB 26), BT549 (ATCC HTB122) and Normal Human Breast Epithelial cell line (MCF-10A) (ATCC CRL-10317) are cultured as per ATCC recommendations. MDA-MB-231cells were cultured in DMEM/F-12 (Lonza, Basel, Switzerland) supplemented with FBS (10%), insulin (10μg/ml), non-essential amino acids, sodium pyruvate, penicillin (100 U/ml) and streptomycin (0.1 mg/ml). BT-549 cells were cultured in RPMI (Lonza, Basel, Switzerland), with FBS (10%), insulin (10μg/ml), and penicillin (100 U/ml) and streptomycin (0.1 mg/ml). MCF-10A was grown in DMEM/F-12 with 5% horse serum (ATCC, Manassas, VA, USA), insulin (10μg/ml), hydrocortisone (0.5 mg/ml), EGF (20ng/ml), penicillin (100 U/ml) and streptomycin (0.1 mg/ml). All cells were kept at 37°C with 5% CO₂.

Human breast cancer cell lines T-47D, MCF7, HCC1954, MDA-MB-231, and BT-549 were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). Normal breast epithelial cell lines HMEC and MCF-12A were kindly provided by Dr. Shiaw-Yih Lin (University of Texas MD Anderson Cancer Center). MCF7 and MDA-MB-231 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM, Lonza, Walkersville, MD, USA) containing 10% fetal bovine serum (FBS, Sigma-Aldrich Co. LLC. St. Louis, MO, USA), 100 units/ml penicillin, and 100 mg/ml streptomycin. T-47D, HCC1954 and BT-549 were maintained in RPMI-1640 medium (Lonza), containing 10% FBS, 100 units/ml penicillin, and 100 mg/ml streptomycin. HMEC and MCF-12A cells were grown in MEBM supplemented with MEGM Single Quots and cholera toxin (Lonza). All cells were grown at 37 °C in a humidified atmosphere of 5% CO₂.