Cell apoptosis was evaluated by flow cytometry. The cells were harvested 48 hours after treatment with VC, ATO or both. The cells were resuspended at a density of 1×106 cells/ml in 1x binding buffer after three PBS washes. The cells were then stained with FITC-Annexin V and propidium iodide (PI) for 30 minutes at 4oC in the dark using the FITC Annexin V: PE Apoptosis Detection Kit I (BD Biosciences). The cells were analyzed using BD FACSCanto II flow cytometer according to the manufacturer’s instructions to detect early and late apoptosis. All experiments were performed in triplicate.
Fitc annexin 5 pe apoptosis detection kit 1
Manufactured by BD
Sourced in United States
The FITC Annexin V: PE Apoptosis Detection Kit I is a laboratory product designed for the detection and measurement of apoptosis. It provides a method for identifying apoptotic cells through the binding of Annexin V, a protein that interacts with phosphatidylserine, a marker of apoptosis. The kit includes both FITC-conjugated Annexin V and propidium iodide, which enables the differentiation between early apoptotic, late apoptotic, and necrotic cells.
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2 protocols using fitc annexin 5 pe apoptosis detection kit 1
1
Apoptosis Quantification by Flow Cytometry
2
Apoptosis Detection Using Annexin V-FITC
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Cell death was detected using an FITC Annexin V-PE apoptosis detection kit I according to the manufacturer's protocol (BD Biosciences, Bedford, MA, USA), as described previously.18 (link) Briefly, cells were treated with gas-only or plasma jets for 2 or 4 V for 1 s and then incubated further for 24 h. The cells were harvested, washed with PBS, and stained with Annexin V-FITC and propidium iodide (PI). The early and late apoptosis were quantified according to the manufacturer's instructions. Apoptosis was detected using a FACS Canto system (BD Biosciences), with the excitation and emission settings of 488 and 530 nm, respectively.
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