Cellroxs deep red reagent

Intracellular reactive oxygen species (ROS) generation was evaluated by Cell ROXs Deep Red Reagent (Thermo Fisher Scientific, USA). Briefly, the cells grown in 96-well plates and treatment with 10 μM artemisinin for 2 h. After 2 h, media was removed and cells were exposed to 100 μM H₂O₂ for 24 h. Cells were then incubated with Cell ROXs Deep Red Reagent (5μM) in fresh DMEM for 1h in dark. Cells were washed with 1X PBS. The fluorescence was measured by fluorescence microscope with a high content screening system (ArrayScanVTI, Thermo FisherScientific, USA) at excitation 640 nm and the emission 665 nm wave length. ROS level was semi-quantified and percentage of ROS level was normalized with the control group.

Intracellular reactive oxygen species (ROS) levels were assessed by CellROXs Deep Red Reagent (Thermo Fisher Scientific, USA), according to the commonly used protocols in our laboratory [24 (link)]. Briefly, cells grown in 96-well plates were incubated with 1 μM Aβ_1-42 with or without pretreatment with different concentrations of Artemether. Cells were then incubated with CellROXs Deep Red Reagent (5 μM in fresh DMEM for 1 hour in the dark). Fluorescence was measured, and semiquantitative ROS levels and normalized ROS levels are presented as a percentage of the control group.