Apc cy7 conjugated anti cd25

Manufactured by BioLegend

APC/Cy7-conjugated anti-CD25 is a fluorescent-labeled antibody that binds to the CD25 antigen. CD25 is the alpha chain of the interleukin-2 receptor, which is expressed on activated T cells and regulatory T cells. This product can be used for flow cytometric analysis.

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Lab products found in correlation

Bv421 conjugated anti cd3, by BioLegend (2 mentions) Fitc conjugated anti cd4, by BioLegend (2 mentions) Anti mouse cd16 32, by BioLegend (2 mentions) Il 17a pe cy7, by BioLegend (2 mentions) Percp cy5.5 conjugated anti rorγt, by BD (2 mentions) Alexa fluor 647 conjugated anti foxp3, by BioLegend (2 mentions) Pe conjugated anti cd44, by BioLegend (1 mentions)

Close spelling variants of this product

Anti-CD25–APC-Cy7 APC-conjugated anti-CD25 CD25-APC-Cy7 Anti-CD25-APC CD25-APC APC-Cy7

2 protocols using apc cy7 conjugated anti cd25

Multiplex Immune Cell Phenotyping

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Fc receptors were blocked with anti-mouse CD16/32 (BioLegend, clone: 93), and surface markers were stained with BV421-conjugated anti-CD3 (BioLegend, clone: 145-2C11), FITC-conjugated anti-CD4 (BioLegend, clone: RM4-5), Alexa Fluor 647-conjugated anti-CD11b (BioLegend, clone: M1/70), PE-conjugated anti-Gr1 (BioLegend, clone: RB6-8C5), PE-conjugated anti-CD44 (BioLegend, clone: IM7), PerCP/Cy5.5-conjugated anti-CD62L (BioLegend, clone: MEL-14), and APC/Cy7-conjugated anti-CD25 (BioLegend, clone: 3C7). After fixing and perminization, intracellular molecules, including cytokines and transcription factors, were stained with PE/Cy7-IL-17A (BioLegend, clone: TC11-18H10.1), PerCP/Cy5.5-conjugated anti-RORγt (BD, clone: Q31-378), Alexa Fluor 647-conjugated anti-FOXP3 (BioLegend, clone:150D), PerCP/Cy5.5-conjugated anti-GM-CSF (BioLegend, clone: MP1-22E9), and Alexa Fluor 647-conjugated anti-TNF-α (BioLegend, clone: MP6-XT22).

Kwon O.C., Lee E.J., Chang E.J., Youn J., Ghang B., Hong S., Lee C.K., Yoo B, & Kim Y.G. (2018). IL-17A+GM-CSF+ Neutrophils Are the Major Infiltrating Cells in Interstitial Lung Disease in an Autoimmune Arthritis Model. Frontiers in Immunology, 9, 1544.

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Multicolor Flow Cytometry for T Cell Phenotyping

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Anti-mouse CD16/32 (BioLegend, clone: 93) was used to block Fc receptors, and the surface markers were stained the following antibodies: FITC-conjugated anti-CD4 (BioLegend, clone: RM4-5), BV421-conjugated anti-CD3 (BioLegend, clone: 145-2C11), and APC/Cy7-conjugated anti-CD25 (BioLegend, clone: 3C7). After fix and permeabilization, cytokines and transcription factors were stained with the following antibodies: PerCP/Cy5.5-conjugated anti-RORγt (BD Biosciences, clone: Q31-378), PE/Cy7-IL-17A (BioLegend, clone: TC11-18H10.1), and Alexa Fluor 647-conjugated anti-FOXP3 (BioLegend, clone: 150D).

Lim D.H., Lee E.J., Kwon O.C., Hong S., Lee C.K., Yoo B., Youn J., Kim T.H, & Kim Y.G. (2019). Effect of tumor necrosis factor inhibition on spinal inflammation and spinal ankylosis in SKG mice. Scientific Reports, 9, 18000.

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