Cell free circulating DNA was extracted in duplicate from 2 ml of plasma by using the QIAamp Circulating Nucleic Acid Kit (Qiagen AG, Basel, Switzerland) according to the manufacturer’s protocol. Absorbed DNA was eluted with 60 µl of provided elution buffer. The synthetic DNA RT-SPCY-T02 (Eurogentec, Angers, France) was added to the plasma to function as positive control for circulating DNA extraction. According to the manufacturer’s protocol, 2 ul of a tenfold diluted RT-SPCY-T02 was added to 2 ml of plasma. Reference buccal samples were extracted using the QIAamp DNA Mini Kit (Qiagen AG, Basel, Switzerland) according to the manufacturer’s protocol and eluted in 100 µl final volume. Both genomic and circulating DNA samples were stored at − 20 °C. DNA was extracted using the QIAamp DNA Mini Kit (Qiagen AG Switzerland) according to the manufacturer’s guidelines and quantified using the kit QuantiFiler Trio on a QuantStudio™ 5 System (Life Technologies Europe, Zug, Switzerland). The commercial DNAs CEPH 1347-02, Control DNA 007 (Life Technologies Europe, Zug, Switzerland), 2800 M Control DNA (Promega, Dübendorf, Switzerland) were genotyped as a reference controls for allele designation.
Rt spcy t02
Manufactured by Eurogentec
Sourced in France
The RT-SPCY-T02 is a laboratory equipment designed for reverse transcription and specific amplification. It is capable of converting RNA into complementary DNA (cDNA) and selectively amplifying target sequences.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rt spcy t02
1
Circulating Cell-free DNA Extraction
2
Extraction and Quantification of Circulating Cell-Free DNA
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Cell-free circulating DNA was extracted in duplicate from 2 ml of plasma by using the QIAamp Circulating Nucleic Acid Kit (Qiagen AG, Basel, Switzerland) according to the manufacturer's protocol. Absorbed DNA was eluted with 60 µl of provided elution buffer. The synthetic DNA RT-SPCY-T02 (Eurogentec, Angers, France) was added to the plasma to function as positive control for circulating DNA extraction. According to the manufacturer's protocol, 2 µl of a 10-fold diluted RT-SPCY-T02 was added to 2 ml of plasma. Reference buccal samples were extracted using the QIAamp DNA Mini Kit (Qiagen) according to the manufacturer's protocol and eluted in 100 µl final volume. Both genomic and circulating DNA samples were stored at -20 °C.
Fetal DNA quantity and percent was measured in the subset of plasma samples from pregnancies of male fetuses. The forensic kit Investigator Quantiplex HYres TM assay (Qiagen) and an AB 7500 Real-Time PCR system (Life Technologies Europe, Zug, Switzerland) was used according to the manufacturer's protocol; data were analyzed using the HID
Fetal DNA quantity and percent was measured in the subset of plasma samples from pregnancies of male fetuses. The forensic kit Investigator Quantiplex HYres TM assay (Qiagen) and an AB 7500 Real-Time PCR system (Life Technologies Europe, Zug, Switzerland) was used according to the manufacturer's protocol; data were analyzed using the HID
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