Log In Sign up
The largest database of trusted experimental protocols

Anti mouse il 12 p40

Manufactured by BioXCell
Visit Supplier

Anti-mouse IL-12 p40 is a lab equipment product that targets the p40 subunit of the interleukin-12 (IL-12) cytokine in mouse samples. It is used for the detection and quantification of IL-12 p40 in various biological applications.

Automatically generated - may contain errors

Lab products found in correlation

Anti mouse ifn γ, by BioXCell (2 mentions)

Spelling variants of this product

Anti-IL-12 (7 citations) IL-12 (5 citations) Anti-mouse IL-12 (3 citations) Anti-mouse IL-12/IL-23 p40 (3 citations)

2 protocols using anti mouse il 12 p40

1

Quantification of IL-12 and IFN-γ in LLC Tumors

Check if the same lab product or an alternative is used in the 5 most similar protocols
LLC tumor tissue sections were prepared as previously described (Oh et al., 2017 (link)). Briefly, tumor tissues were fixed in 2% paraformaldehyde overnight, followed by embedding in optimum cutting temperature (O.C.T.; Tissue-Tek) compound, and were cut into 14-μm sections. The sections were blocked with 1.0% BSA in PBST (PBS+ 0.1% Tween 20) and incubated with anti-mouse IL-12 p40 (Bio X Cell) or anti-mouse IFN-γ (Bio X Cell) at room temperature for 1 hr. After wash 3 times with PBST, the sections were stained with goat anti-rat IgG H&L (Alexa Fluor® 568) at room temperature for 1 hr. The sections were next counterstained with DAPI and mounted on glass slides. Images were acquired with a LSM880 microscope with Airyscan and FAST Airyscan High Resolution and 32-channel spectral detectors. Relative fluorescence intensity of IL-12 or IFN-γ was quantified by normalizing integrated fluorescence intensity of IL-12 or IFN-γ to the total cell numbers of macrophages, DCs, or CD8+ T cells per mg of tumor.
Wang Y., Song M., Liu M., Zhang G., Zhang X., Li M.O., Ma X., Zhang J.J, & Huang X.Y. (2021). Fascin inhibitor increases intratumoral dendritic cell activation and anti-cancer immunity. Cell reports, 35(1), 108948.
+ Open protocol
+ Expand
2

Quantification of IL-12 and IFN-γ in LLC Tumors

Check if the same lab product or an alternative is used in the 5 most similar protocols
LLC tumor tissue sections were prepared as previously described (Oh et al., 2017 (link)). Briefly, tumor tissues were fixed in 2% paraformaldehyde overnight, followed by embedding in optimum cutting temperature (O.C.T.; Tissue-Tek) compound, and were cut into 14-μm sections. The sections were blocked with 1.0% BSA in PBST (PBS+ 0.1% Tween 20) and incubated with anti-mouse IL-12 p40 (Bio X Cell) or anti-mouse IFN-γ (Bio X Cell) at room temperature for 1 hr. After wash 3 times with PBST, the sections were stained with goat anti-rat IgG H&L (Alexa Fluor® 568) at room temperature for 1 hr. The sections were next counterstained with DAPI and mounted on glass slides. Images were acquired with a LSM880 microscope with Airyscan and FAST Airyscan High Resolution and 32-channel spectral detectors. Relative fluorescence intensity of IL-12 or IFN-γ was quantified by normalizing integrated fluorescence intensity of IL-12 or IFN-γ to the total cell numbers of macrophages, DCs, or CD8+ T cells per mg of tumor.
Wang Y., Song M., Liu M., Zhang G., Zhang X., Li M.O., Ma X., Zhang J.J, & Huang X.Y. (2021). Fascin inhibitor increases intratumoral dendritic cell activation and anti-cancer immunity. Cell reports, 35(1), 108948.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!