Blocking grade non fat dry milk

Manufactured by Bio-Rad

Sourced in United States

Blocking grade non-fat dry milk is a laboratory reagent used for the preparation of blocking solutions in various immunological and molecular biology techniques. It is a purified and standardized form of non-fat dry milk that is suitable for use in these applications.

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Lab products found in correlation

Enhanced chemiluminescence detection system, by Thermo Fisher Scientific (2 mentions) Hybond ecl membrane, by GE Healthcare (1 mentions) Protease inhibitor, by Merck Group (1 mentions) Bcip nbt phosphatase substrate, by LGC (1 mentions) Bis tris sds page gel, by Thermo Fisher Scientific (1 mentions) Imagequant, by GE Healthcare (1 mentions) Nupage 4 12 bis tris gel, by Thermo Fisher Scientific (1 mentions) Dc protein assay, by Bio-Rad (1 mentions) Gapdh, by Merck Group (1 mentions) Cellytic nuclear extraction kit, by Merck Group (1 mentions) Pvdf membrane, by Pall Corporation (1 mentions)

Spelling variants of this product

Non-fat dry milk (305 citations) Non-fat milk (201 citations) Dry milk (17 citations) Non-fat blocking grade milk (5 citations) Blocking grade milk (4 citations) Non-fat blocking milk (2 citations)

3 protocols using blocking grade non fat dry milk

Western Blot Analysis of Treated Cells

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Cells were treated with compound 1 at varying
concentrations for 24 h, and protein lysates were then prepared in RIPA lysis
buffer containing protease inhibitors (Sigma, St Louis, MO, USA). Volumes of
clarified protein lysate containing equal amounts of protein (50
μg) were separated on 10–12% sodium
dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and
electrophoretically (90 min at 100 V) transferred to a Hybond-ECL membrane (GE
Healthcare, Piscataway, NJ, USA). Blots were then blocked for 1 h in 1 ×
TBST (10 mM Tris-HCl, pH 8.0, 150 mM NaCl, and 0.05% Tween-20)
containing 5% blocking grade non-fat dry milk (Bio-Rad, Hercules, CA,
USA), and then incubated overnight with primary antibody at 4 °C. Blots
were then washed for three times in 1 × TBST and incubated for 2 h at
room temperature with HRP-conjugated goat anti-rabbit or anti-mouse IgG
secondary antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA).
Immunoreactive bands were visualized using an enhanced chemiluminescence
detection system (Thermo Scientific, Rockford, IL, USA).

Chen L.X., Xia G.Y., He H., Huang J., Qiu F, & Zi X.L. (2016). New withanolides with TRAIL-sensitizing effect from Physalis pubescens L.. RSC advances, 6(58), 52925-52936.

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Protein Detection in Vaccine Preparations

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Protein content of vaccine preparations was quantitated by BCA Protein Assay kit (Pierce, Rockford, IL). Twenty μg of protein extract from whole LVS, LVS-G, LVS-R and HK-LVS bacteria, lysed in 1% SDS, were electrophoresed on a 4–12% Bis-Tris SDS-PAGE gel (Life Technologies) and transferred to a nitrocellulose membrane. Immediately after protein transfer, the membranes were washed with Ponceau S concentrate (Sigma) for 3 minutes. The membranes were then washed with distilled water for 3 minutes, and images of the stained membranes were acquired. Membranes were then blocked with 5% Blocking Grade Non-Fat Dry Milk (Bio-Rad) in Tris-Buffered Saline with 0.1% Tween 20 (TBST). Pooled sera, collected after two or six weeks from mice vaccinated with LVS or heat-killed LVS, or collected after three days after LVS challenge, were used as primary antibodies at a 1:1000 dilution in TBST. A conjugated anti-mouse IgG antibody (Sigma-Aldrich) at a 1:4000 dilution in TBST was used to detected antigen binding antibodies. Blots were developed with BCIP/NBT Phosphatase substrate (Kirkegaard & Perry Laboratories) for 15 minutes, after which the reaction was stopped with water, per the manufacturer’s instructions.

De Pascalis R., Mittereder L., Chou A.Y., Kennett N.J, & Elkins K.L. (2015). Francisella tularensis Vaccines Elicit Concurrent Protective T- and B-Cell Immune Responses in BALB/cByJ Mice. PLoS ONE, 10(5), e0126570.

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Quantitative Western Blot Analysis of Nrf2 and Related Proteins

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The protein was extracted with CelLytic™ NuCLEAR™ Extraction Kit (Sigma) following the manufacturer’s instruction. The Bio-Rad DC Protein Assay (Bio-Rad Laboratories, Hercules, CA) was used to determine protein concentration. Target proteins were measured by western blot analysis with the following antibodies: rabbit antibodies against Nrf2 (1:200 dilution, Abcam), GCLM (1:1000 dilution, abcam), GCLC (1:800 dilution, abcam), and GAPDH (1:5000 dilution, Sigma). Aliquots containing 50μg proteins were heated for 5 min at 55°C and were loaded on NuPAGE 4-12% Bis-Tris gel (Life Technologies, Grand Island, NY), and then transferred to a PVDF membrane (Pall Life Sciences, Ann Arbor, MI). The membrane was blocked in TBS-T (Thermo) containing 5% blocking grade non-fat dry milk (Bio-Rad), and incubated overnight with primary antibodies at 4°C. After washing, the membrane was incubated with HRP-conjugated goat anti-rabbit secondary antibody (Cell signals) at 1:3000 dilution at RT for 2 hours. Immunoreactive bands were visualized using an enhanced chemiluminescence detection system (Thermo Scientific, Rockford, IL) (8 (link)). Densitometric measurements were done with Image Quant (Molecular Dynamics). GAPDH bands, as a housekeeping protein, were used to normalize the expression of the target proteins.

Masuda Y., Vaziri N.D., Takasu C., Li S., Robles L., Pham C., Le A., Vo K., Farzaneh S.H., Stamos M.J, & Ichii H. (2014). Salutary effect of pre-treatment with an Nrf2 inducer on ischemia reperfusion injury in the rat liver. Gastroenterology and hepatology, 1(1), 1-7.

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