The chemicals for chromatography were previously reported [39 (link),48 (link),49 (link)]. Single-stranded salmon sperm DNA was obtained from Sigma-Aldrich (St. Louis, MO, USA). Quinoin from the seeds of C. quinoa and PD-L4 from the leaves of P. dioica were isolated as previously reported [34 (link),39 (link)]. The nuclease-treated rabbit reticulocyte lysate system was purchased from Promega (Madison, WI, USA).
The medium and the other chemicals were from Sigma Chemical Co. (St. Louis, MO, USA). The RPMI 1640 medium, fetal bovine serum (FBS), penicillin, streptomycin and trypsin were purchased from GIBCO BRL (Barcelona, Spain). The Z-VAD-fmk (pan-caspase inhibitor carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone) named Z-VAD was purchased from R&D Systems (Abingdon, UK).
Buffer A: 5 mM Na-phosphate, pH 7.2, containing 0.14 M NaCl; buffer B: 10 mM Na-acetate, pH 4.0; and buffer C: 5 mM Na-phosphate, pH 7.2.
The medium and the other chemicals were from Sigma Chemical Co. (St. Louis, MO, USA). The RPMI 1640 medium, fetal bovine serum (FBS), penicillin, streptomycin and trypsin were purchased from GIBCO BRL (Barcelona, Spain). The Z-VAD-fmk (pan-caspase inhibitor carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone) named Z-VAD was purchased from R&D Systems (Abingdon, UK).
Buffer A: 5 mM Na-phosphate, pH 7.2, containing 0.14 M NaCl; buffer B: 10 mM Na-acetate, pH 4.0; and buffer C: 5 mM Na-phosphate, pH 7.2.