The MRI data was collected on a BioSpec 70/20 (7T field strength, 20 cm bore diameter) small animal MR system with a Paravision 360 console (Bruker BioSpin MRI, Ettlingen, Germany). T2w anatomical MRI was acquired using TurboRARE 3D with the following parameters: TR = 350 ms, echo spacing = 12 ms, six echoes, TEeff = 36 ms, field of view (FOV) 24 × 9.6 × 12 mm, matrix size 400 × 160 × 200, resolution = 60 × 60 × 60 μm, scan time was 33 min.
Paravision 360
Manufactured by Bruker
Sourced in Germany
Paravision 360 is a comprehensive software platform for the acquisition, processing, and analysis of magnetic resonance imaging (MRI) data. It provides a versatile and user-friendly interface for researchers and scientists working with preclinical and small-animal MRI systems.
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Lab products found in correlation
7 protocols using paravision 360
1
High-resolution 7T small animal MRI
2
T2-weighted and Diffusion-weighted MRI of Brain Tumors
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MR images were acquired at 7.1 T on a Bruker Avance300 spectrometer equipped with the Micro2.5 microimaging probe at room temperature. T2W morphological images were acquired using a standard RARE (Rapid Acquisition with Refocused Echoes) sequence with the following parameters: TR = 4000 ms, TE = 40.57 ms, RARE factor = 24, flip angle = 180°, number of averages = 4, FOV = 30 mm x 30 mm, slice thickness 1 mm, matrix size 256 x 256, and spatial resolution = 0.117 × 0.117 mm/pixel. Diffusion-Weighted MR Images (DWI-MRI) were acquired using Spin Echo sequences with TE = 27 ms, TR = 750 ms, number of averages = 1, FOV = 30 x 30 mm, slice thickness = 1 mm, matrix size 128 x 128, spatial resolution = 0.234 × 0.234 mm/pixel. Six different B-values were used (0, 150, 250, 500, 755, and 1000 s/mm2). Apparent diffusion constant (ADC) maps were calculated by fitting DW-MRI signal intensity as a function of B-values using Bruker ParaVision360 software.36 (link) ADC maps were obtained by superimposing the ADC values of the tumor ROI onto T2w MRI (Fiji-Image software).
3
Multimodal MRI Assessment of BBB Permeability and Hemorrhage
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MRI was performed using a Bruker 11.7 Tesla small animal scanner (Bruker BioSpin MRI, Ettlingen, Germany) equipped with a cross coil configuration with a mouse body resonator for transmit and a mouse surface coil for receive. Images were acquired using ParaVision 360 (Bruker BioSpin MRI). Permeability of the BBB was determined with a T1 weighted (T1w) sequence (2D RARE sequence, RARE factor = 2, repetition time (TR) 250 ms, echo time (TE) 6.7 ms, 1 mm slice thickness, 1 mm interslice distance, 13 images, field of view (FOV) = 2 x 2 cm, matrix = 384 x 384, number of acquisitions (NA) = 6) following intravenous injection of Gd-HPDO3A (Prohance®, 0.5 μmol/g mouse body weight). Hemorrhage was (2D FLASH sequence, flip angle (FA) = 15º, repetition time (TR) 250 ms, echo time (TE) 15 ms, 1 mm slice thickness, 1 mm interslice distance, 13 images, field of view (FOV) = 2 x 2 cm2, matrix = 192 x 192, number of acquisitions (NA) = 4).
4
NMR and MRI Data Analysis
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NMR spectra were quantified by manual integration after manual phase correction, line broadening, and baseline correction (MestReNova 14.2.2, Mestrelab Research S.L.). MRI images were analyzed using the manufacturer’s software (Paravision 360, Bruker).
5
Optimized 1H-MRS and ASL Perfusion Imaging
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The parameter settings for 1 H-MRS were as follows: RT/TE = 4808/33 ms, FOV = 20 × 20 mm2, image size = 128 × 128, and slice = 1. The ASL perfusion image was obtained using Bruker Para Vision 360 software.
6
Multimodal MRI Imaging of Metacestodes
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MRI scanning was performed using a small-animal MRI Facility (Bruker PharmaScan 70/16 US, Germany) with the Paravision 360 software platform. The scanning coil is mouse body coilRF RES 300 1H 075/040 QSN TR. The mice were anesthetized by inhalation with 2% ~ 3% isoflurane (RWD, Shenzhen) during scanning. The body temperature of mice was kept constant throughout the experiment using a thermoregulated water circulation system, and the oxygenation level of the mice was monitored by a pulse oximeter. The scan protocols for axial images were performed using the T2_TurboRARE as described previously (40 (link)). In brief, echo time (TE) = 25 ms, repetition time (TR) = 2100 ms, slice thickness = 1.0 mm, field of view (FOV) = 35 mm×30 mm, scanning time = 17 min 42s; T1_FLASH_flc: TE = 3 ms, TR = 300 ms, flip angle = 90°, slice thickness = 1.0 mm, FOV = 35 mm×30 mm, scanning time = 5min 45s. The maximum diameter of metacestodes was quantified and analyzed using T1 Imaging and T2 imaging of Sante MRI Viewer V3.0.
7
High-Field MRI Characterization of Tumor
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MR images were acquired at 7.1T on a Bruker Avance300 spectrometer equipped with the Micro2.5 microimaging probe at room temperature. T 2W morphological images were acquired using a standard RARE (Rapid Acquisition with Refocused Echoes) sequence with the following parameters: TR=4000 ms, TE=40.57 ms, RARE factor=24, ip angle=180°, number of averages=4, FOV=30 mm x 30 mm, slice thickness 1 mm, matrix size 256x256, and spatial resolution=0.117x0.117 mm/pixel. Diffusion-Weighted MR Images (DWI-MRI) were acquired using a Spin Echo sequence with TE=27 ms, TR=750 ms, number of averages=1, FOV=30x30 mm, slice thickness=1 mm, matrix size 128x128, and spatial resolution=0.234x0.234 mm/pixel. Six different B-values were used (0, 150, 250, 500, 755 and 1000 s/mm 2 ). Apparent diffusion constant (ADC) maps were calculated by tting DW-MRI signal intensity as a function of B-values using Bruker ParaVision360 software [27] . ADC maps were obtained by superimposing the ADC values of the tumor ROI onto T 2w MRI (Fiji-ImageJ software).
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