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Meta morph image analysis software

Manufactured by Olympus
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Sourced in Japan

Meta-Morph is an image analysis software developed by Olympus. It provides tools for processing and analyzing digital images. The software's core function is to assist users in extracting and quantifying relevant data from images.

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Lab products found in correlation

Rhodamine 6g, by Merck Group (2 mentions) Carboxyfluorescein succinimidyl ester (cfse), by Merck Group (2 mentions) Ix81 microscope, by Olympus (1 mentions) Anti fade mounting solution, by Electron Microscopy Sciences (1 mentions) Dsu confocal microscope, by Olympus (1 mentions) Anti fade gold, by Thermo Fisher Scientific (1 mentions)

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Image analysis software

4 protocols using meta morph image analysis software

1

Immunofluorescence Staining of BMDCs

Cited 2 times
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The immunofluorescence staining was described previously[6 (link), 8 (link), 15 (link)]. Briefly, harvested BMDCs were seeded in 8-well chamber slides at 2×105 cells/chamber and cultured with 10% FBS RPMI-1640 medium overnight. Cells were starved for 2 hours in pre-warmed SFM and then treated with CpG-B (1μM) for 30 minute or left untreated. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.2% Triton X-100, blocked with 5% BSA in PBS, and stained with primary anti-DNA-PKcs and anti-TRAF3 Abs in PBS with 2% BSA at 4°C overnight. Alexa fluorescence-conjugated secondary Abs (Alexa 488 or 594) (Lifetechnologies, NY, USA) was added and incubated for 2 hours. Slides were mounted with anti-fade mounting solution (Electron Microscopy Sciences, PA, USA) and observed under an IX81 Olympus microscope with 60X oil immersion objective powered by 1.6x magnification. The images were captured by an ORCA R2 CCD mono camera and processed by the Metamorph Image Analysis Software (Olympus, Japan).
Ma C., Spies N.P., Gong T., Jones C.X, & Chu W.M. (2015). Involvement of DNA-PKcs in the Type I IFN Response to CpG-ODNs in Conventional Dendritic Cells in TLR9-Dependent or -Independent Manners. PLoS ONE, 10(3), e0121371.
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2

Amylo-Glo Staining of ReN Cells

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The fixed 3D thin-layer cultures of ReN cells were washed 3 times with saline and incubated with 0.001% (v/v) Amylo-Glo solution for 5 minutes in a dark environment. The cells were then washed with saline followed by three washings with DDW. To avoid fluorescence quenching, a drop of anti-fade gold (Life Technologies) was added on top of the stained cells. The Amylo-Glo fluorescence was measured by Olympus DSU confocal microscope with Metamorph image analysis software (Olympus).
Choi S.H., Kim Y.H., Hebisch M., Sliwinski C., Lee S., D’Avanzo C., Chen J., Hooli B., Asselin C., Muffat J., Klee J.B., Zhang C., Wainger B.J., Peitz M., Kovacs D.M., Woolf C.J., Wagner S.L., Tanzi R.E, & Kim D.Y. (2014). A three-dimensional human neural cell culture model of Alzheimer’s disease. Nature, 515(7526), 274-278.
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3

Intravital Imaging of Cerebral Platelets

Cited 1 time
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Intravital fluorescence imaging of cerebral was performed at 4 h after 20-min tHI insults in β-thalassemia and littermate control mice, as previously reported (Sun et al., 2017 (link)). Briefly, isolated platelets (1 × 108) from a wildtype mouse were stained with 90 μM carboxyfluorescein succinimidyl ester (CFSE, Sigma-Aldrich), followed by tail-vein injection at 5 min before intravital imaging. Rhodamine 6G (Sigma-Aldrich, 0.02%) was further injected via an infusion pump to label circulating leukocytes. The leukocytes and platelets were visualized on an inverted fluorescence microscope (AX70, Olympus). The randomly selected intravital images were captured 20 frames/min for 2 min by a CCD camera (CoolSNAP HQ2, Photometrics) and analyzed with the Meta-Morph image analysis software (Olympus). Adherent cells were defined as having a stationary interaction on the venular wall for longer than 3 s.
Sun Y.Y., Yao H.W., Chen H.R., Chen C.W., Kinkaid M.M, & Kuan C.Y. (2022). Stroke propensity in the Th3+/ mouse model of β-thalassemia intermedia. Neurobiology of disease, 171, 105802.
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4

Intravital Imaging of Cerebral Platelets

Cited 1 time
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Intravital fluorescence imaging of cerebral was performed at 4 h after 20-min tHI insults in β-thalassemia and littermate control mice, as previously reported (Sun et al., 2017 (link)). Briefly, isolated platelets (1 × 108) from a wildtype mouse were stained with 90 μM carboxyfluorescein succinimidyl ester (CFSE, Sigma-Aldrich), followed by tail-vein injection at 5 min before intravital imaging. Rhodamine 6G (Sigma-Aldrich, 0.02%) was further injected via an infusion pump to label circulating leukocytes. The leukocytes and platelets were visualized on an inverted fluorescence microscope (AX70, Olympus). The randomly selected intravital images were captured 20 frames/min for 2 min by a CCD camera (CoolSNAP HQ2, Photometrics) and analyzed with the Meta-Morph image analysis software (Olympus). Adherent cells were defined as having a stationary interaction on the venular wall for longer than 3 s.
Sun Y.Y., Yao H.W., Chen H.R., Chen C.W., Kinkaid M.M, & Kuan C.Y. (2022). Stroke propensity in the Th3+/ mouse model of β-thalassemia intermedia. Neurobiology of disease, 171, 105802.
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