5979 mass selective detector

Gas chromatography–mass spectrometry (GC–MS) analysis was performed in accordance with our previous developed method with slight modification (Supriady et al., 2015 (link)). The machine used was Agilent Technologies 6980N (GC) equipped with 5979 Mass Selective Detector (MS), HP-5MS (5% phenyl methyl siloxane) capillary column of dimensions 30.0 m × 250 μm × 0.25 μm and used helium as carrier gas at 1 mL/min. The column temperature was programmed initially at 40°C for 10 min, followed by an increase of 3°C/min to 250°C and was kept isothermally for 5 min. The MS was operating at 70 eV. The constituents were identified by comparison of their mass spectral data with those from NIST 05 Spectral Library.

GC-MS chemical profiling was conducted based on a previously developed protocol [122 (link)]. The analysis was conducted using Agilent Technologies 6980 N (GC) equipped with 5979 Mass Selective Detector (MS), HP-5MS (5% phenyl methyl siloxane) capillary column of dimensions 30.0 m × 250 μm × 0.25 μm using helium as the carrier gas at 1 mL/ min. The experiment was initiated by maintaining the column temperature at 40 °C for 10 mins, followed by an increase of 3 °C per min to 250 °C and was kept isothermal for 5 min. The MS was operating at 70 eV. Comparison of the mass spectral between the detected chemical constituents and the standards available in W9 N11 MS library was performed to identify the chemical constituents present in the extract.

GC-MS analysis was performed to profile the potential bioactive compounds present in MUM292 extract according to Supriady et al. [71 (link)]. The analysis was conducted using the Agilent Technologies 6980N (GC) equipped with 5979 Mass Selective Detector (MS), HP-5MS (5% phenyl methyl siloxane) capillary column of dimensions 30.0 m × 250 μm × 0.25 μm and helium as carrier gas at 1 mL/min. For the initial 10 min, the temperature of column was maintained at 40°C. The temperature was then increased by 3°C/min until 250°C and kept isothermally for 5 min. The MS was operating at 70 eV. The mass spectra of detected chemical constituents were compared to those available from W9N11 MS library.

GC-MS analysis was performed according to previously developed method with slight modification (Supriady et al., 2015 (link)). The analysis was conducted using Agilent Technologies 6980N (GC) equipped with 5979 Mass Selective Detector (MS), with HP-5MS (5% phenyl methyl siloxane) capillary column of dimensions 30.0 m × 250 μm × 0.25 μm and helium as carrier gas at 1 mL/min. The column temperature was programmed initially at 40°C for 10 min, followed by an increase of 3°C/min to 250°C and was kept isothermally for 5 min. The MS was operating at 70 eV. The constituents were identified by comparison of their mass spectral data with those from NIST 05 Spectral Library.

The profiling of chemical constituents in the methanolic extract of strain MUSC 14 was carried out based on method described by Tan et al. [54 (link)]. Agilent Technologies 6980N was coupled with a 5979 Mass Selective Detector. The HP-5MS (5% phenyl methyl siloxane) capillary column of dimensions 30.0 m × 250 μm × 0.25 μm was used as helium gas carrier at 1 mL/min. Initial temperature of column was set at 40°C with a gradual increase of 3°C every minute until it achieved the maximum of 250°C and then stationed for an intermediate 5 minutes. MS was set to operate at 70 eV. The detected constituents were identified by comparing their mass spectral data with standard compounds from NIST 05 spectral Library.

GC-MS analysis was performed in accordance with our previous developed method with slight modification (Supriady et al., 2015 (link); Ser et al., 2015a (link)). The machine used was Agilent Technologies 6980N (GC) equipped with 5979 Mass Selective Detector (MS), HP-5MS (5% phenyl methyl siloxane) capillary column of dimensions 30.0 m × 250 × 0.25 μm and used helium as carrier gas at 1 mL/min. The column temperature was programmed initially at 40°C for 10 min, followed by an increase of 3°C/min to 250°C and was kept isothermally for 5 min. The MS was operating at 70 eV. The constituents were identified by comparison of their mass spectral data with those from NIST 05 Spectral Library.

The GC-MS analysis was performed on the methanolic extract MUSC 125 following the method of Tan, et al. [46 (link)] which utilized an Agilent Technologies 6980N fitted with 5979 Mass Selective Detector and a HP-5 MS (5% phenyl methyl siloxane) capillary column to carry helium gas at the rate of 1 mLs⁻¹. Heat was gradually applied until 40 °C was reached whilst keeping it constant for 10 min; then, it was increased by 3 °C every minute until the peak temperature of 250 °C was reached keeping, it constant for 5 another minutes. The mass spectrometry was functioning at 70 eV. Individual compounds detected by GC-MS were matched with the NIST 05 reference library.