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Au400 automatic biochemistry analyzer

Manufactured by Olympus
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Sourced in Japan

The AU400 is an automatic biochemistry analyzer designed to perform a wide range of clinical chemistry tests. It is capable of processing multiple samples and reagents simultaneously, providing efficient and reliable results for laboratory testing.

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Lab products found in correlation

Total antioxidant status kit, by Randox (1 mentions) Ransel kit, by Randox (1 mentions)

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AU400 automatic analyzer Automatic biochemistry analyzer AU400 analyzer Automatic analyzer AU400

11 protocols using au400 automatic biochemistry analyzer

1

Evaluating Diabetes Biomarkers in Serum

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All detection kits were purchased from Beyotime Institute of Biotechnology (Beijing, China). Fasting serum insulin (FINS) and GSP levels were measured by using an enzyme-linked immunosorbent assay (ELISA) kit according to their manufacturer’s instruction.
The levels of ALT, AST, Scr, BUN, serum total proteins (TP), albumin (ALB), total cholesterol (TC), and triglyceride (TG) in serum were detected by Olympus AU400 automatic biochemistry analyzer (Olympus, Tokyo, Japan). The SOD activity, MDA and GSP levels in serum were determined with SOD, MDA and GSP detection kits according to the manufacturer’s protocols.
The OGTT procedure was performed at the end of 0, 3, 20 and 48 weeks after induction of diabetes. After food restricted for 6 h, blood samples were collected from snipped tails by tail milking at 0, 30, 60, and 120 min after administration of d-glucose (20% solution; 2 g/kg BW) by stomach tube. 1, 3, 20 and 44 weeks after induction of diabetes, fasting plasma glucose (FBG) and fasting serum insulin (FINS) were determined as described previously [12 (link)]. The OGTT-area under curve (OGTT-AUC) was calculated by the following formula:
Homoeostasis model assessment (HOMA) of beta-cell function (HOMA-B) and assessment-insulin resistance (HOMA-IR) were calculated by the HOMA method using the following equations:

Xu M., Sun B., Li D., Mao R., Li H., Li Y, & Wang J. (2017). Beneficial Effects of Small Molecule Oligopeptides Isolated from Panax ginseng Meyer on Pancreatic Beta-Cell Dysfunction and Death in Diabetic Rats. Nutrients, 9(10), 1061.
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2

Lipid and Inflammatory Biomarker Profiling

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Fasting serum glucose, high‐sensitivity C‐reactive protein (hs‐CRP), total cholesterol (CHO), triacylglycerol (TG), high‐density lipoprotein cholesterol (HDL‐C), and low‐density lipoprotein cholesterol (LDL‐C) levels were measured by Olympus AU400 automatic biochemistry analyzer (Olympus, Tokyo, Japan). All detection kits were purchased from InTec PRODUCTS, INC. (Xiamen, China).
Tan X., Gao L., Cai X., Zhang M., Huang D., Dang Q, & Bao L. (2021). Vitamin D3 alleviates cognitive impairment through regulating inflammatory stress in db/db mice. Food Science & Nutrition, 9(9), 4803-4814.
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3

Biocompatibility Evaluation of Enzyme Capsules

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To evaluate the biocompatibility of enzyme capsules, the blood samples were collected on Day 8 and centrifuged at 3000 rpm for 10 min, and the supernatants were subjected to the measurement of serum biochemical indicators including aspartate transaminase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), blood urea nitrogen (BUN) and creatinine (CREA) on an Olympus AU-400 Automatic Biochemistry Analyzer.
Liang X., Wen K., Chen Y., Fang G., Yang S, & Li Q. (2022). Oral Administration of Therapeutic Enzyme Capsule for the Management of Inflammatory Bowel Disease. International Journal of Nanomedicine, 17, 4843-4860.
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4

Biochemical Markers in Liver Tissues

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The levels of ALT, AST, TP, ALB, GLB, TBIL, TC, and TG in serum were detected by Olympus AU400 automatic biochemistry analyzer (Olympus, Tokyo, Japan). The SOD activity, GSH, GSSG and MDA levels in liver tissues were determined with SOD, MDA and GSH/GSSG detection kits according to the manufacturer’s protocols. All detection kits were purchased from Beyotime Institute of Biotechnology (Beijing, China).
Cai X., Bao L., Wang N., Xu M., Mao R, & Li Y. (2016). Dietary Nucleotides Supplementation and Liver Injury in Alcohol-Treated Rats: A Metabolomics Investigation. Molecules, 21(4), 435.
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5

Antioxidant System Profiling

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The antioxidant system was analyzed by determining the total antioxidant capacity (TAC) and the glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities (Mehdipour et al., 2017 (link)). They were determined spectrophotometrically by using Randox kits (RANDOX Laboratories Ltd.) and an Olympus AU 400 automatic biochemistry analyzer (Olympus, Tokyo, Japan), converting absorbance to specific units with calibration curves. TAC was assessed by adding the reactive provided in the kit and measuring the absorbance at 600 nm, converting absorbance to mmol/L. GPx was measured in the presence of oxidized glutathione (cumene hydroperoxide) and NADPH. The oxidized glutathione was then reduced by GPx with a concomitant oxidation of NADPH to NADP+, determining the decrease in absorbance at 340 nm. SOD determination is based on the degree of inhibition of the oxidation of 2-(4 -iodophenyl)-3-(4- nitrophenol)-5-phenyltetrazoliumchloride (INT) to the red formazan dye by superoxide radicals (produced by a xanthine/xanthine oxidase system). One unit of SOD prevents reduction of INT by 50% under the conditions of the assay. The absorbance was recorded at 505 nm.
Najafi A., Taheri R.A., Mehdipour M., Martínez-Pastor F., Rouhollahi A.A, & Nourani M.R. (2018). Improvement of post-thawed sperm quality in broiler breeder roosters by ellagic acid-loaded liposomes. Poultry Science, 98(1), 440-446.
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6

Antioxidative System in Seminal Plasma

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The antioxidative system in seminal plasma was monitored by determining the superoxide dismutase, glutathione peroxidase, and total antioxidant capacities. The superoxide dismutase activity, glutathione peroxidase activity, and total antioxidant capacity of the seminal plasma samples were each determined spectrophotometrically on an Olympus AU 400 automatic biochemistry analyzer (Olympus, Tokyo, Japan) using commercial Randox ® kits (Randox Laboratories Ltd., London, UK). Namely, the superoxide dismutase activity was determined by a commercial Ransod ® kit (Cat. No. SD124; Ransod Control reference material SD126, Randox Laboratories Ltd., London, UK), the activity of glutathione peroxidase by a commercial Ransel ® kit (Cat. No. RS504; Ransel Control reference material SC692, Randox Laboratories Ltd., London, UK), and the total antioxidant capacity concentration was determined with a commercial Total Antioxidant Status ® kit (Cat. No. NX 2332; Randox Total Antioxidant Control Cat. No. NX 2331, Randox Laboratories Ltd., London, UK). Superoxide dismutase activity was expressed in U/mL, glutathione peroxidase activity in IU/L, and the concentration of total antioxidant capacity in mmoL/L of seminal plasma.
Galić I., Dragin S., Stančić I., Maletić M., Apić J., Kladar N., Spasojević J., Grba J, & Kovačević Z. (2022). Effect of an Antioxidant Supplement Combination on Boar Sperm. Animals : an Open Access Journal from MDPI, 12(10), 1301.
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7

Lipid Quantification in Seminal Plasma

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Commercially available kits for the determinations of TG, TC, LDL and HDL were purchased from Shanghai Zhicheng Biotechnology Co., Ltd., China. Calibration and quality control products were purchased from Randox Laboratories Ltd., Northern Ireland, United Kingdom. Determination of lipids in seminal plasma was carried out using Olympus AU400 automatic biochemistry analyzer (Olympus Optical Co. Ltd., Japan). The sample with higher lipid level exceeding the linear range of the kit should be diluted with normal saline and the diluted volume was calculated.
Lu J.C., Jing J., Yao Q., Fan K., Wang G.H., Feng R.X., Liang Y.J., Chen L., Ge Y.F, & Yao B. (2016). Relationship between Lipids Levels of Serum and Seminal Plasma and Semen Parameters in 631 Chinese Subfertile Men. PLoS ONE, 11(1), e0146304.
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8

Antioxidant System Assessment Protocol

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The antioxidant system was examined by the assessment of GPx, TAC, and SOD levels [18 (link)]. These variables were assessed spectrophotometrically by Randox™ kits (RANDOX Laboratories Ltd.) and an Olympus AU 400 automatic biochemistry analyzer (Olympus, Tokyo, Japan).
Mehdipour M., Daghigh Kia H., Najafi A., Mohammadi H, & Álvarez-Rodriguez M. (2020). Effect of crocin and naringenin supplementation in cryopreservation medium on post-thaw rooster sperm quality and expression of apoptosis associated genes. PLoS ONE, 15(10), e0241105.
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9

Biomarker Profiles in Mouse Serum

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The levels of IL-6, IL-8, TNF-α, CCL2, CRP, IL-10, SDF-1α and NO in mice serum were measured by an enzyme linked immunosorbent assay (ELISA), according to the kit’s instructions. The levels of serum ALB, PA and TRF were detected by Olympus AU400 automatic biochemistry analyzer (Olympus, Tokyo, Japan). The ROS contents in serum and hydroxyproline levels in skin tissue were determined with ROS and hydroxyproline detection kits according to the manufacturer’s protocols. All detection kits were purchased from Beyotime Institute of Biotechnology (Beijing, China).
Li D., Li L., Xu T., Wang T., Ren J., Liu X, & Li Y. (2018). Effect of Low Molecular Weight Oligopeptides Isolated from Sea Cucumber on Diabetic Wound Healing in db/db Mice. Marine Drugs, 16(1), 16.
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10

Serum Biomarker Profiling in Wound Healing

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The levels of albumin (ALB), prealbumin (PA) and transferrin (TRF) in serum were determined by Olympus AU400 automatic biochemistry analyzer (Olympus, Tokyo, Japan). The level of hydroxyproline (Hyp) in wound tissue was detected according to the kit’s instructions. The levels of interleukin (IL)-8, IL-10, tumor necrosis factor (TNF)-α, chemokine (C-C motif) ligand 2 (CCL2), C-reactive protein (CRP) and SDF-1α in mice serum were measured by an enzyme linked immunosorbent assay (ELISA). All detection kits were purchased from Beyotime Institute of Biotechnology (Beijing, China).
Li D., Ren J.W., Xu T., Li L., Liu P, & Li Y. (2021). Effect of bovine bone collagen oligopeptides on wound healing in mice. Aging (Albany NY), 13(6), 9028-9042.
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