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3 protocols using 2 3 dimethylfuran

1

Furan Derivatives Procurement and Analysis

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The furan standard was procured from Alfa Aesar Co. (Ward hill, MA, USA), while 3-methylfuran was from Acros Co., (Carlsbad, CA, USA), and 2-pentylfuran from Tokyo Chemical Industry Co. (Tokyo, Japan). The other furan derivative standards, including 2-methylfuran, 2,3-dimethylfuran, 2,5-dimethylfuran, 2-ethylfuran, furfural, furfuryl alcohol, 2-butylfuran, and 2-acetylfuran, were from Sigma-Aldrich (St. Louis, MO, USA). The internal standard d4-furan was from Dr. Ehrenstorfer GmbH Co. (Augsburg, Germany). The HPLC-grade methanol was from Merck (Darmstadt, Germany), and the sodium chloride was from Uni Region Bio-Tech (Los Altos, CA, USA). The sodium chloride was purchased from UNI-ONWARD CORP (New Taipei City, Taiwan).
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2

Analytical Standards for Amino Acids, Sugars, and Furan Compounds

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Analytical standards for twenty-two amino acids (list in Table 2, norvaline and sarcosine), six sugars (D-fructose, D-glucose, sucrose, Dmaltose, β-lactose and phenyl-b-D-glucopyranoside), furan and five alkylfurans (2-methylfuran, 3-methylfuran, 2-ethylfuran, 2,5-dimethylfuran and 2,3-dimethylfuran) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Thiophene and 2-methylthiophene were bought from VWR (Radnor, PA, USA) and acrylamide from Chiron (Trondheim, Norway). For labelled standards analogous, furan and alkyls (furan-d 4 , 2-methylfuran-d 6 , 3-methylfuran-d 3 and 2,5-dimethylfuran-d 3 ) were bought at Toronto Research Chemicals (Toronto, ON, Canada), while acrylamide (acrylamide-d 3 ) comes from Chiron.
Methanol HPLC grade, boric acid, trifluoroacetic acid (TFA), hexamethyldisilazane (HMDS) and Carrez Solution II (30% ZnSO4) were
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3

Quantification of Furan Derivatives

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Furan standard was purchased from Alfa Aesar Co. (Ward hill, MA, USA), while 3-methylfuran was from Acros Co., (Carlsbad, CA, USA), and 2-pentylfuran from Tokyo Chemical Industry Co. (Tokyo, Japan). The other furan derivative standards including 2-methylfuran, 2,3-dimethylfuran, 2,5-dimethylfuran, 2-ethylfuran, furfural, furfuryl alcohol, 2-butylfuran and 2-acetylfuran were from Sigma–Aldrich (St. Louis, MO, USA). Internal standard d4-furan was from Dr. Ehrenstorfer GmbH Co. (Augsburg, Germany). The HPLC-grade solvents methanol and petroleum ether were from Merck (Darmstadt, Germany). Deionized water was produced by a Milli-Q water purification system (Bedford, MA, USA). Sodium chloride, ninhydrin, 3,5-dinitrosalicylic acid, d-dextrose, potassium sodium tartrate 4-hydrate, and glycine was purchased from Sigma–Aldrich (St. Louis, MO, USA).
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