Water was purified to Milli-Q water grade using an in-house Milli-Q® Reference Water Purification System (Millipore). Collagen–fluorescein (FITC) conjugate type I from bovine tendon was purchased from BioVision Inc. (Milpitas, CA, USA) The EnzChek™ gelatinase/collagenase assay kit, containing DQTM gelatin from pig skin fluorescein conjugate, was obtained from InvitrogenTM by Thermo Fisher Scientific Inc. (Waltham, MA, USA) (lot 2,174,593 and 2,281,586). GibcoTM Dulbecco’s phosphate-buffered saline (no calcium, no magnesium, pH 7.0 to 7.3; DPBS (2.67 mM KCl, 1.47 mM KH2PO4, 137.93 mM NaCl, 8.06 mM Na2HPO4-7H2O)) was purchased from Thermo Fisher Scientific Inc. Meanwhile, 2-propanol HPLC grade was purchased from VWR International. Acetonitrile HPLC-R (ACN), formic acid 99%, ULC/MS–CC/SFC (FA) and trifluoroacetic acid HPLC grade (TFA) were obtained from Biosolve B.V. Difluoroacetic acid for LC-MS (LiChropur, ≥97.5% (GC); DFA) was purchased from Sigma-Aldrich.
Trifluoroacetic acid hplc grade tfa
Manufactured by Biosolve
Trifluoroacetic acid HPLC grade (TFA) is a chemical compound commonly used as a mobile phase additive in high-performance liquid chromatography (HPLC) analysis. It is a colorless, volatile, and corrosive liquid with a pungent odor. TFA HPLC grade is a highly purified version of trifluoroacetic acid that is suitable for sensitive HPLC applications.
Automatically generated - may contain errors
Lab products found in correlation
Milli q reference water purification system, by Merck Group (2 mentions)
Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (1 mentions)
Hoechst 33342, by Thermo Fisher Scientific (1 mentions)
Dulbecco s phosphate buffered saline, by Thermo Fisher Scientific (1 mentions)
Varespladib, by Merck Group (1 mentions)
Trizma base, by Thermo Fisher Scientific (1 mentions)
2 protocols using trifluoroacetic acid hplc grade tfa
1
Collagen-based Enzymatic Assay Protocol
2
Detailed Reagent Preparation Protocol
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Ultrapure water was obtained through purification using a Milli-Q® Reference Water Purification System (Millipore). Dulbecco's phosphate buffered saline (no calcium, no magnesium, pH 7.0–7.3; PBS) was purchased from Thermo Fisher Scientific Inc. Tris-HCl buffer (50 mM, pH 7.4) was prepared with Trizma® base in ultrapure water and the pH was as adjusted using 1.0 M HCl. Egg yolk from chicken (powder), Trizma® base (≥99.9 %), hydrochloric acid (ACS reagent, 37 %; HCl) and Triton™ X-100 (BioXtra) were obtained from Sigma-Aldrich. Hoechst 33342 (trihydrochloride, trihydrate - 10 mg/mL solution in water; Hoechst) nucleic acid stain was obtained from Invitrogen™ by Thermo Fisher Scientific Inc., Gibco™. For the neutralisation assays, varespladib (Sigma-Aldrich) was used as a PLA2-inhibitor. Acetonitrile HPLC-R (ACN) and trifluoroacetic acid HPLC grade (TFA) were obtained from Biosolve B.V.
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