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Dm4000m microscope

Manufactured by Leica
Sourced in Germany

The DM4000M is a microscope designed for routine laboratory applications. It features a sturdy, ergonomic design and provides high-quality optical performance for a wide range of sample types. The microscope is equipped with various illumination options and supports a range of magnification levels to accommodate diverse research and analysis requirements.

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5 protocols using dm4000m microscope

1

Plant DNA Extraction and Sequencing

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Retsch MM400 Ball Mill (Laichi co., Berlin, Germany); PTC200PCR (Bio‐Rad, Berkeley, CA, USA); GelDox XR gel imaging system (Bio‐Rad, USA); DYY‐8C Electrophoretic apparatus (Beijing Liuyi Instrument, China); BI3730XL sequenator (Applied Biosystems, Foster City, CA, USA); KRQ‐300P manual climatic box (ChongQing YinHe Test Instrument, China); Plant DNA Extraction Kit (TIANGEN Biotech Co., Chengdu, China); 2 × Taq PCR Master Mix (TIANGEN Biotech Co.,); primer was compounded by Sangon Co., China. Shimadzu; DM4000M microscope (Leica, Frankfurt, Germany); TruSeq® DNA PCR‐Free Sample Preparation Kit (Illumina Co., San Diego, CA, USA); QIAquick gum recovery kit (QIAGEN, Duesseldorf, Germany); Phusion® High‐Fidelity PCR Master Mix with GC Buffer (New England Biolabs, USA); High‐efficient enzyme (New England Biolabs, Boston, MA, USA).
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2

Structural Characterization of Photodetectors

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Raman spectroscopy (LabRAM HR800, He–Ne laser excitation at 532 nm) was used for structural characterization. Optical images were acquired by a Leica DM4000 M microscope. Field-emission scanning electron microscopy (FE-SEM, FEI Nova Nano-SEM 450) and transmission electron microscopy (TEM, Tecnai G220 U-TWIN) were used for investigating the morphology and structure of the samples. The current–time characteristics of the photodetector were measured by a low-temperature cryogenic probe station (Lake Shore CRX-6.5 K), a semiconductor parameter analyzer (Keithley 4200-SCS), and a light source (Energetiq EQ-1500).
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3

Thin Film Fabrication and Characterization of N-Heterotriangulenes

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Optical microscopy images were recorded using a Leica DM 4000 M microscope equipped with a Leica DFC 320 camera. AFM images and corresponding height profiles were measured in non‐contact mode using a JPK NanoWizard4 microscope. NEXAFS spectra were recorded in partial electron yield mode at the HE‐SGM beamline at BESSY II, Berlin, Germany. Electron diffraction patterns were recorded using a Philips CM 30 transmission electron microscope at an acceleration voltage of 300 kV. Charge‐transport characteristics of fabricated OFETs were recorded at a Keithley SCS 4200 parameter analyzer under atmospheric conditions. Thin films were prepared via liquid‐liquid processing. Beakers were sonicated in acetone and isopropanol for 15 minutes subsequently and rinsed thoroughly with deionized (Millipore) water afterwards. 15 mL of Millipore water were then given into a beaker and solution of the respective N‐heterotriangulene concentration was added dropwise to form a floating lens on the water surface. Beakers were stored at RT or 278 K respectively until the solvent had evaporated. Resulting thin films were transferred from the water surface onto silicon wafers using a home‐made vertical lifting system. Single crystals of 3 suitable for X‐ray crystallographic analysis were successfully grown by solvent diffusion of hexanes into a solution of compound 3 in CH2Cl2.
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4

Phase Behavior Characterization by HSM

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To qualitatively investigate the phase behavior of the formulations, they were investigated by HSM at a heating rate of 2 K·min−1 in the same temperature range as for the DSC measurements. Experiments were performed on a Linkam THMS600 hot stage (Tadworth, UK) mounted to a Leica DM4000M microscope (Wetzlar, Germany). A sample of 5–10 mg was placed onto the heating block and HSM images were taken in time frames of 60 s.
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5

Quantification of Citrus Flavonoids

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They were as follows: Retsch MM400 Ball Mill(Laichi co., Germany); PTC200PCR(BIO-Rad, American); GelDox XR gel imaging system (BIO-Rad, American); DYY-8C Electrophoretic apparatus (Beijing Liuyi Instrument, China); BI3730XL sequenator (Applied Bio-systems, American); KRQ-300P manual climatic box (ChongQing YinBe Test Instrument, China); Plant DNA Extraction Kit (Tiangen Biotech Co., China); 2×Taq PCR MasterMix (Tiangen Biotech Co., China); primer was compounded by Sangon Co., China. Shimadzu; LC-20AT HPLC (Shimadzu Co., Japan); DM4000M microscope (Leica, Germany); Agilent 8453 ultraviolet and visible spectrophotometer (Agilent Technologies Co., American); hesperidin (MUST-12041206); narirutin (MUST-14081915); hesperetin (MUST-14100914); naringenin (MUST-14112310), four reference substance were bought from Chengdu Mansite biological technology co. LTD, purity>98.0%. The other reagents were analytically pure.
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