The terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) was performed using a cy5-TUNEL staining kit (R&D Systems). The positive controls used 10 minutes’ incubation at room temperature with 1500 U/ml DNAse1 in 50 mM Tris pH 7.5, 10 mM MgCl2 and 1 mg/ml BSA.
Cy5 tunel staining kit
Manufactured by R&D Systems
Sourced in United States
The Cy5-TUNEL staining kit is a reagent used to detect DNA fragmentation, a hallmark of apoptosis or programmed cell death. The kit utilizes the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) method, where Cy5-labeled nucleotides are incorporated into the DNA strand breaks, allowing for fluorescent detection and visualization of apoptotic cells.
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Lab products found in correlation
2 protocols using cy5 tunel staining kit
1
TUNEL Assay for Cell Death
2
Immunocytochemistry and TUNEL Assay
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Immunocytochemistry and terminal deoxynucleotidyl transferase-mediated digoxigenin-dUTP nick-end labeling (TUNEL) assay staining were done using an immunocytochemistry staining kit (Dako, Carpinteria, CA, USA) and a Cy5-TUNEL staining kit (R&D Systems, Beijing, China), respectively. The rabbit anti-CDK5 antibody was purchased from Abcam (Shanghai, China).
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