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Epicatechin gallate

Manufactured by Fujifilm
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Sourced in Japan

Epicatechin gallate is a polyphenolic compound found in green tea. It is a type of catechin, a class of antioxidants. Epicatechin gallate is used in laboratory research as a reference standard and analytical tool.

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Lab products found in correlation

Epigallocatechin gallate, by Fujifilm (4 mentions) Epicatechin ec, by Fujifilm (2 mentions) Epigallocatechin, by Fujifilm (2 mentions) Acetonitrile, by Avantor (2 mentions) Methanol, by Avantor (2 mentions) Retinoic acid, by Merck Group (1 mentions) Pancreatin from porcine pancreas, by Merck Group (1 mentions) Lipase from porcine pancreas, by Merck Group (1 mentions) Sodium acetate, by Samchun (1 mentions) Epicatechin, by Fujifilm (1 mentions) Pepsin from porcine gastric mucosa, by Merck Group (1 mentions) Acetic acid, by Avantor (1 mentions) Ethyl acetate, by Samchun (1 mentions) Dichloromethane dcm, by Samchun (1 mentions) Trichloroacetic acid, by Merck Group (1 mentions) Gallic acid, by Merck Group (1 mentions) Ascorbic acid, by Fujifilm (1 mentions) Bile acid, by Merck Group (1 mentions) Lipase, by Merck Group (1 mentions) Phosphoric acid, by Junsei (1 mentions)

5 protocols using epicatechin gallate

1

Procyanidin Compounds Synthesis and Purification

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(−)-Catechin (C), (−)-epicatechin (EC), (−)-epicatechin gallate (ECG), and (−)-epigallocatechin gallate (EGCG) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Procyanidin B1 (EC-C) was purchased from Extrasynthese (Lyon, France). Procyanidin B2 (EC-EC) was purchased from Toronto Research Chemicals Inc. (Toronto, Canada). Procyanidin C1 (EC-EC-EC) and retinoic acid (RA) were purchased from Sigma-Aldrich Co. LLC (St. Louis, MO, USA). Gallic acid (GA) was purchased from Funakoshi Co. Ltd. (Tokyo, Japan). All chemicals were used without further purification. Procyanidin B3 (C–C), procyanidin B4 (C-EC), procyanidin B4-GA (C-ECG), EC-CG, C–C–C, and C–C-ECG were synthesized according to our previous study [14 (link)].
Nakamura Y., Tsuchiya T., Hara-Chikuma M., Yasui M, & Fukui Y. (2020). Identification of compounds in red wine that effectively upregulate aquaporin-3 as a potential mechanism of enhancement of skin moisturizing. Biochemistry and Biophysics Reports, 24, 100864.
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2

Analytical Standards for Tea Polyphenols

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The analytical standards of (−)-epigallocatechin-3-gallate (EGCG), (−)-epigallocatechin gallate (EGCG), (−)-epicatechin gallate (ECG), (−)-epigallocatechin (EGC), and (−)-epicatechin (EC) were purchased from Wako (Osaka, Japan). Benzo[α]pyrene (B[α]P), α-amylase from human saliva, pepsin, from porcine gastric mucosa, bile extract from porcine, lipase from porcine pancreas, and pancreatin from porcine pancreas were obtained from Sigma Aldrich (St. Louis, MO, USA). Water, methanol, acetonitrile, and acetic acid with a grade for ultra-performance liquid chromatography (UPLC) were purchased from J.T. Baker (Phillipsburg, NJ, USA.) and Sigma Aldrich (St. Louis, MO, USA), respectively. Sodium acetate, dichloromethane (DCM), and ethyl acetate were obtained from Samchun Chemicals (Seoul, Korea).
Jeong K.H., Lee H.J., Park T.S, & Shim S.M. (2019). Catechins Controlled Bioavailability of Benzo[a]pyrene (B[α]P) from the Gastrointestinal Tract to the Brain towards Reducing Brain Toxicity Using the In Vitro Bio-Mimic System Coupled with Sequential Co-Cultures. Molecules, 24(11), 2175.
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3

Antioxidant Assays of Tea Compounds

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Aluminum nitrate nonahydrate, aluminium chloride, 2,2-diphenyl-1-picryl-hydrazyl (DPPH), ferric chloride, ferrous sulfate, gallic acid, Folin–Ciocalteu’s reagent, 3,5-dinitrosalicylic acid, hydrogen peroxide, potassium dihydrogen phosphate, potassium ferricyanide, sodium salicylate, sodium carbonate, sodium nitrite, trichloroacetic acid, amino acids [alanine (Ala), aspartic acid (Asp), arginine (Arg), histidine (His), gamma-butyric acid (GABA), lysine (Lys), glutamic acid (Glu), serine (Ser), threonine (Thr), theanine (Thea), tyrosine (Tyr)], and caffeine were obtained from Sigma Chemical Co. (St. Louis, MO, USA). Catechins [(+)-catechin (C), (-)-epicatechin (EC), (-)-epigallocatechin (EGC), (-)-epicatechingallate (ECG), and (-)-epigallocatechin gallate (EGCG)] and theaflavins [theaflavin (TF), theaflavin-3-gallate (TF3G), and theaflavin-3’-gallate (TF3’G)] were obtained from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). All other chemicals and solvents used were of standard analytical grade.
Tong T., Liu Y.J., Kang J., Zhang C.M, & Kang S.G. (2019). Antioxidant Activity and Main Chemical Components of a Novel Fermented Tea. Molecules, 24(16), 2917.
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4

Enzymatic Assay of Tannase Substrates

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Methyl gallate (MG), epicatechin gallate (ECg) epigallocatechin gallate (EGCg) catechin gallate (Cg), and gallocatechin gallate (GCg), used as substrates for enzyme assay of tannases, were obtained from Wako Pure Chemical Industries., Ltd. (Osaka, Japan). In addition, (−)-epigallocatechin-3-O–(3-O–methyl) gallate (EGCg3″Me) was purchased from Nagara Science (Gifu, Japan). Structures of substrates are shown in Additional file 1: Figure S1. All substrates were dissolved in 50 mM phosphate buffer (pH 6.8) containing 1% ascorbic acid (Wako) at a final concentration of 0.5 mM or 1 mM.
Ueda S., Nomoto R., Yoshida K.I, & Osawa R. (2014). Comparison of three tannases cloned from closely related lactobacillus species: L. Plantarum, L. Paraplantarum, and L. Pentosus. BMC Microbiology, 14, 87.
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5

Quantification of Catechins and Flavonoids

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Epigallocatechin (EGC), epigallocatechin-3-gallate (EGCG), epicatechin gallate (ECG), and epicatechin (EC) were obtained from Wako (Osaka, Japan). Monopotassium phosphate (KH2PO4), dipotassium phosphate (K2HPO4), α-amylase, pepsin, lipase, bile acid, pancreatin, sodium bicarbonate (NaHCO3), fisetin, myricetin, kaempferol, quercetin, and formic acid were purchased from Sigma Aldrich (St. Louis, MO, USA). Sodium hydroxide and hydrogen chloride (HCl) were purchased from Daejung chemicals and metals (Gyeonggi-do, South Korea). Triple distilled water was purchased from Ultra 370 series, Gyeonggi-do, South Korea. Phosphoric acid was purchased from Junsei Chemical (Tokyo, Japan). Acetonitrile, water, methanol, and HPLC-grade distilled water were purchased from J.T.Baker (Phillipsburg, NJ, USA). Dimethyl sulfoxide (DMSO) with 50% MeOH was purchased from Duksan (Ansan, Gyeonggi-do, South Korea).
Oh J.H., Lee C.Y., Lee Y.E., Yoo S.H., Chung J.O., Rha C.S., Park M.Y., Hong Y.D, & Shim S.M. (2021). Profiling of In Vitro Bioaccessibility and Intestinal Uptake of Flavonoids after Consumption of Commonly Available Green Tea Types. Molecules, 26(6), 1518.
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