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Cresyl violet acetate

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Cresyl violet acetate is a chemical compound commonly used in laboratory settings. It is a synthetic dye that has applications in various staining techniques, particularly in histology and microscopy. Cresyl violet acetate is known for its ability to stain specific cellular structures, which can aid in the visualization and analysis of biological samples.

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Lab products found in correlation

Transmission electron microscope, by Hitachi (2 mentions) Pannoramic desk scanner, by 3DHISTECH (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions) H 7650, by Hitachi (1 mentions)

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Cresyl violet (26 citations)

7 protocols using cresyl violet acetate

1

Hippocampal Nissl Body Quantification

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The slices were stained using 0.5% cresyl violet acetate (Beyotime, China). The sections were scanned using a transmission electron microscope (Hitachi, Japan). The integrated optical density of Nissl bodies in the hippocampus, CA1, CA3 and DG regions was quantified by Image Pro Plus 6.0 software.
Gao Q., Wang Y., Wang X., Fu S., Zhang X., Wang R.T, & Zhang X. (2019). Decreased levels of circulating trimethylamine N-oxide alleviate cognitive and pathological deterioration in transgenic mice: a potential therapeutic approach for Alzheimer’s disease. Aging (Albany NY), 11(19), 8642-8663.
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2

Hippocampal Nissl Body Analysis

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The sections were stained by 0.5% cresyl violet acetate (Beyotime, China) and photographed using a transmission electron microscope (TEM)(Hitachi, Japan). The integrated optical density (IOD) of Nissl bodies in the hippocampus was calculated using Image Pro Plus 6.0 software.
Wang Q.J., Shen Y.E., Wang X., Fu S., Zhang X., Zhang Y.N, & Wang R.T. (2020). Concomitant memantine and Lactobacillus plantarum treatment attenuates cognitive impairments in APP/PS1 mice. Aging (Albany NY), 12(1), 628-649.
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3

Nissl Staining for Ischemic Brain Injury

Cited 1 time
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Nissl staining was performed as previously reported (Lin et al., 2021 (link)). Twenty four hours after reperfusion, the rats (n = 5) were anesthetized and perfused transcardially with cold saline and 4% paraformaldehyde (PFA, Sigma-Aldrich, United States). The brains were rapidly removed, immersed in the fixative for 48 h and then embedded in paraffin and micro-sectioned into coronal slices of 5 μm thickness. The coronal brain sections were stained using 0.5% cresyl violet acetate (Beyotime, Beijing, China). The severity of neural damage was monitored by counting the number of normal neurons in infarct tissues under a Pannoramic DESK scanner from 3D-HISTECH (Hungary). The cells that contained the Nissl stain in the cytoplasm, loose chromatin and prominent nucleoli were considered to be normal neurons. Stained cells in five lesioned regions of the ischemic cortex were counted randomly.
Zhang S., Jiang X., Wang Y., Lin K., Zhang Z., Zhang Z., Zhu P., Ng M.L., Qu S., Sze S.C, & Yung K.K. (2021). Protective Effect of An-Gong-Niu-Huang Wan Pre-treatment Against Experimental Cerebral Ischemia Injury via Regulating GSK-3β/HO-1 Pathway. Frontiers in Pharmacology, 12, 640297.
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4

Quantitative Analysis of Nissl Bodies

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The mice brains were removed after anesthesia and fixed in 4% paraformaldehyde. The sections (4 μm) were stained using 0.5% cresyl violet acetate (Beyotime, China). The integrated optical density (IOD) of Nissl bodies was quantified by using Image J software (Version 1.48, National Institutes of Health).
Wang C., Zhu M.H., Sun N., Shen W., Jiang N., Zhao Q.S., Zhang Y.X., Huang Y, & Zhou W.X. (2022). Isorhynchophylline ameliorates stress-induced emotional disorder and cognitive impairment with modulation of NMDA receptors. Frontiers in Neuroscience, 16, 1071068.
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5

Hippocampal Nissl Body Analysis

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The slices were stained by 0.7% cresyl violet acetate (Beyotime, China) and taken photos by a transmission electron microscope (Hitachi, Japan). The integrated optical density (IOD) of hippocampal Nissl bodies was analyzed by IPP 6.0 software.
Liu J., Zhang T., Wang Y., Si C., Wang X., Wang R.T, & Lv Z. (2020). Baicalin ameliorates neuropathology in repeated cerebral ischemia-reperfusion injury model mice by remodeling the gut microbiota. Aging (Albany NY), 12(4), 3791-3806.
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6

Analyzing Nissl Bodies in Hippocampal Regions

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The sections were stained using 0.5% cresyl violet acetate (Beyotime, Beijing China). Stained sections were scanned using a transmission electron microscope (H-7650, Hitachi, Tokyo, Japan). The integrated optical density (IOD) of Nissl bodies in the CA1 and CA3 regions was quantified using the Image Pro Plus 6.0 software.
Wang J.H., Lei X., Cheng X.R., Zhang X.R., Liu G., Cheng J.P., Xu Y.R., Zeng J., Zhou W.X, & Zhang Y.X. (2016). LW-AFC, a new formula derived from Liuwei Dihuang decoction, ameliorates behavioral and pathological deterioration via modulating the neuroendocrine-immune system in PrP-hAβPPswe/PS1ΔE9 transgenic mice. Alzheimer's Research & Therapy, 8, 57.
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7

Quantifying Hippocampal Neuron Survival

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The left hemisphere coronal sections were stained using 0.5% Cresyl Violet Acetate (Beyotime, Beijing, China). The severity of neural damage was assessed by counting the number of surviving neurons in the CA1, CA3, and dentate gyrus (DG) areas of the hippocampus under a microscope (Nikon, Tokyo, Japan). Cells with cytoplasmic Nissl staining, loose chromatin, and prominent nucleoli were considered healthy neurons. The data in Fig. 3B–D represent the number of cells per field.
Lin K., Sze S.C., Liu B., Zhang Z., Zhang Z., Zhu P., Wang Y., Deng Q., Yung K.K, & Zhang S. (2020). 20(S)-protopanaxadiol and oleanolic acid ameliorate cognitive deficits in APP/PS1 transgenic mice by enhancing hippocampal neurogenesis. Journal of Ginseng Research, 45(2), 325-333.
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