The following ink formulations were used: Dextran ink: 10 g/ml Dextran (Sigma-Aldrich) was dissolved in 75:25 v:v, water: isopropanol, TPU ink: TPU 15 wt% Elastollan 35A (BASF) dissolved in 4:1 v:v tetrahydrofuran:dimethylformamide, CB:TPU ink: TPU 15 wt% Elastollan 35A, 5 wt% carbon black (Vulcan XC72R, Cabot) dissolved in 4:1 v:v tetrahydrofuran:dimethylformamide. Ag:Pa ink: 50 g Silver flakes (5–8 μm) mixed with 5.2 g 30 wt% versamid 973 solution (BASF) dissolved in pentanol, mixed with an additional 2.72 g of pentanol. Soft PDMS Ink: SE1700 (Dow-Corning) with 1:25 curing agent based ratio was applied for cantilever covers, micro-pins and micro-wells. Rigid PDMS ink: SE1700 (Dow-Corning), with 1:10 curing agent weight ratio, mixed 1:5 wt:wt with Sylgard 184 1:10 curing agent weight ratio (Dow-Corning) is used for wells and covers, or as gasket adhesive below PLA or ABS wells and covers.
Se1700
Manufactured by Dow
Sourced in United States
The SE1700 is a high-performance laboratory equipment designed for precise analytical tasks. Its core function is to provide accurate and reliable measurements through advanced sensing and data processing capabilities. The SE1700 is engineered to deliver consistent and reproducible results, making it a valuable tool for scientific research and industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Sylgard 184, by Dow (4 mentions)
Elastollan 35a, by BASF (2 mentions)
Solidworks, by Dassault Systèmes (1 mentions)
Glass slide, by Corning (1 mentions)
Dragon skin 30, by Dow (1 mentions)
Phosphate buffer saline (pbs), by Thermo Fisher Scientific (1 mentions)
3 butyn 1 ol, by Merck Group (1 mentions)
W 450d sonifier, by Emerson (1 mentions)
Inkredible, by Cellink (1 mentions)
10 protocols using se1700
1
Inkjet-Printed Electronics Formulation
2
Inkjet-Printed Electronics Formulation
3
3D Printing Conductive Polymer Hydrogels
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3D printing of the conducting polymer ink and the PDMS ink (SE 1700, Dow Corning) were conducted based on a custom-designed 3D printer based on a Cartesian gantry system (AGS1000, Aerotech)18 (link) with various size of nozzles (200- and 100-µm nozzles from Nordson EFD; 50-µm nozzles from Fisnar; 30-µm nozzles from World Precision Instrument). Printing paths were generated by CAD drawings (SolidWorks, Dassault Systèmes) and converted into G-code by a commercial software package (CADFusion, Aerotech) and custom Python scripts to command the x-y-z motion of the printer head. The detailed printing paths are provided in Supplementary Figs. 6 , 11 , and 12 .
After printing, the 3D-printed conducting polymer was dried at 60 °C for 24 h followed by multiple cycles of annealing at 130 °C (3 cycles with 30 min per each cycle) to yield pure PEDOT:PSS33 (link). To achieve constrained drying of 3D-printed conducting polymers in thickness direction, the 3D-printed conducting polymers were placed on a glass substrate and dry-annealed. The dry-annealed 3D-printed conducting polymer was further equilibrated in PBS to be converted into a hydrogel state.
After printing, the 3D-printed conducting polymer was dried at 60 °C for 24 h followed by multiple cycles of annealing at 130 °C (3 cycles with 30 min per each cycle) to yield pure PEDOT:PSS33 (link). To achieve constrained drying of 3D-printed conducting polymers in thickness direction, the 3D-printed conducting polymers were placed on a glass substrate and dry-annealed. The dry-annealed 3D-printed conducting polymer was further equilibrated in PBS to be converted into a hydrogel state.
4
PDMS Well Array Fabrication for Bioprinting
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PDMS was prepared by blending two silicone elastomers, including a low-viscosity PDMS material Sylgard 184 and a shear-thinning PDMS material SE 1,700 (Dow Corning, Auburn, MI, USA) which is used to dilute Sylgard 184 for desired rheological properties. Both SE 1,700 and Sylgard 184 base materials were mixed with their curing agents for at least 10 min in a 10:1 (base: curing agent) ratio by weight before blending and were placed in a vacuum desiccator for degassing for 15–20 min just after mixing the base and agent. Then, SE 1,700 and Sylgard 184 were mixed in an optimized ratio and placed in a vacuum desiccator for 10 min. Next, the PDMS mixer was loaded into a 3-cc syringe (barrels syringe, Cellink) at room temperature and centrifuged at 5,000 rpm for 5 min to remove any air bubbles. The proper nozzle at 140 kPa extrusion pressure was used to print the PDMS wells. To be adaptive with different sizes and shapes of the bioprinted structure, three different constructs of PDMS well array were printed. The wells with 5 mm x 5 mm x 3 mm (Length, width, height) dimensions were printed for sphere bioprinted structures, and 10 mm x 10 mm x 3 mm wells were fabricated for printing rectangular bioprinted structures. Also, 15 mm x15mm x 3 mm wells were printed for 2D-drug screening experiments. Once printed, the PDMS well arrays were cured in an oven at 75 °C for 4 h.
5
Rapid Fabrication of PDMS Bioreactor Walls
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Bioreactor walls were fabricated using custom injection stainless steel molds designed to permit rapid casting of the PDMS, SYLGARD 184 (Dow Corning), walls prepared with a 10:1 elastomer-to-catalyst ratio. After casting and curing in an oven at 65°C for at least 8 hours, the resulting PDMS walls were bonded to glass slides using a printed layer of SE1700 (Dow Corning). The PDMS walls were aligned to the printed silicone and pressed firmly into contact with the slide. Assembled devices were cured at 65°C for at least 2 hours to irreversibly bond the walls to the glass surface.
6
Silicone Elastomer Ink Fabrication
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A silicone elastomer ink was prepared by mixing Dragon Skin 30 (Smooth-On) and SE 1700 (Dow Corning) together. Specifically, Dragon Skin 30 part A, Dragon Skin 30 part B, SE 1700 base, and SE 1700 catalyst were added in a 10:10:10:1 weight ratio and mixed thoroughly using a Thinky mixer (AR-100, Thinky). The ink was printed onto a glass slide (Corning) treated with a hydrophobic coating (RainX) into the desired geometry and cured in the oven at 120 °C for 30 min. After curing and cooling, a layer of the tissue adhesive ink (prepared as described above) was printed on top of the silicone layer, following the same geometry. After drying, the patch was removed from the glass slide and evaluated using a standard tensile test.
7
Bacterial Cellulose Membrane Fabrication
8
Fabrication of Superhydrophobic PDMS Surface
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A polydimethylsiloxane adhesive (SE1700) with a curing agent was purchased from Dow Corning, United States 3-Butyn-1-ol (97%) and 1H,1H,2H,2H-perfluorooctyl-trichlorosilane (CF3(CF2)5CH2CH2SiCl3; PFTS, 97%) were purchased from Sigma-Aldrich. Toluene and absolute ethanol were purchased from the Chengdu Kelong Chemical Company, China.
9
Fabrication of Conductive Silicone Elastomers
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Silicone elastomers, SE1700, OE6520 and MS2002 (Dow Corning), were prepared by mixing catalyst and base at a ratio of 1:10, 1:1 and 1:1, respectively, followed by degassing. For electrical fibres, conductive ink was fabricated by mixing platinum powder (particle diameter 0.2-1.8 μm, ChemPur, Germany) with TGME (Merck, KGaA), followed by tip sonication with Branson W-450 D sonifier (1/8" Tapered Microtip, 30% of power, 10 s/ 10 s pulse duration/delay). The platinum content in the suspension was around 15% by weight.
10
Fabrication of PDMS Molds and Posts for Cell Biomechanics
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In order to fabricate the PDMS molds for cell-laden hydrogel casting and posts for the force measurement platform, the 3D bioprinter INKREDIBLE+ from CELLINK® was used. Both structures were made of polydimethylsiloxane (PDMS; Dow Corning SE 1700) with a crosslinker ratio of 1:20 (coming with the base material as a kit), mixed according to the manufacturer's instructions. The designs of the structures were carried out in AutoCAD (v. 2019), exported as .stl files, and transformed into GCode to be 3D printed at a pressure of 220 kPa. After printing, the molds were cured at 80 ºC for 16 h and posts were cured at 37 ºC for 72 h. The walls of the molds were 1 mm high and their inner and outer diameters were 10 mm and 13 mm, respectively. The posts were 3 mm high, 0.5 mm wide and with 2 mm of lateral width, separated by a distance of 9 mm.
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