An individual sequencing library was prepared for each isolate using the NexteraXT DNA sample preparation kit (Illumina, San Diego, CA, USA). Then, isolates were pooled and multiplexed for paired-end sequencing on a single MiSeq instrument, using the MiSeq reagent kit v3 150 bp (Illumina). Libraries were assembled by SeqSphere+ software version 4.1.9 (Ridom GmbH, Muenster, Germany) for automated quality trimming and assembly. The resulting FASTA files were uploaded to the KmerFinder 3.1 on the Center for Genomic Epidemiology and the top Kmer result for each isolate was recorded (18 (link)– (link)20 (link)). Each isolate was also analyzed by the Comprehensive Antibiotic Resistance Database (CARD) and the presence of mecA, mecC, and vanA was determined (17 (link), 21 (link)– (link)23 (link)).
Miseq reagent kit v3 150 bp
Manufactured by Illumina
The MiSeq reagent kit v3 150 bp is a laboratory equipment product designed for use with the MiSeq sequencing system. The kit provides the necessary reagents and consumables to perform 150 base pair sequencing runs on the MiSeq platform.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using miseq reagent kit v3 150 bp
1
Comprehensive Genomics-based Bacterial Profiling
2
Comprehensive Genomics-based Bacterial Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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An individual sequencing library was prepared for each isolate using the NexteraXT DNA sample preparation kit (Illumina, San Diego, CA, USA). Then, isolates were pooled and multiplexed for paired-end sequencing on a single MiSeq instrument, using the MiSeq reagent kit v3 150 bp (Illumina). Libraries were assembled by SeqSphere+ software version 4.1.9 (Ridom GmbH, Muenster, Germany) for automated quality trimming and assembly. The resulting FASTA files were uploaded to the KmerFinder 3.1 on the Center for Genomic Epidemiology and the top Kmer result for each isolate was recorded (18 (link)– (link)20 (link)). Each isolate was also analyzed by the Comprehensive Antibiotic Resistance Database (CARD) and the presence of mecA, mecC, and vanA was determined (17 (link), 21 (link)– (link)23 (link)).
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