Blood samples for PLR analysis were drawn the day before operation and analyzed by a Beckman Coulter LH 780 Analyzer (Pasadena, California, USA). The samples were stored in EDTA-containing tubes. PLR was defined as the absolute platelet count divided by the absolute lymphocyte count. Neutrophil-to-lymphocyte ratio (NLR) was defined as the absolute neutrophil count divided by the absolute lymphocyte count. In a population-based study, reference mean value of PLR and NLR was calculated as 137 ± 102 and 2.8 ± 1.6 consecutively.[2 ]
Lh 780 analyzer
Manufactured by Beckman Coulter
Sourced in United States
The LH 780 Analyzer is a laboratory instrument designed for the analysis of complete blood count (CBC) and white blood cell differential. It utilizes flow cytometry technology to provide accurate and reliable results for a variety of hematological parameters.
Automatically generated - may contain errors
Lab products found in correlation
Elecsys and modular dp, by Roche (2 mentions)
Xe 2100 analyzer, by Sysmex (1 mentions)
Advia 2400 analyzer, by Siemens (1 mentions)
Acl top 700, by Beckman Coulter (1 mentions)
Cobas e411 device, by Roche (1 mentions)
Array 360 system, by Beckman Coulter (1 mentions)
Compound microscope, by Nikon (1 mentions)
Cobas 702, by Roche (1 mentions)
Au5800 analyzer, by Beckman Coulter (1 mentions)
Coulter access 2, by Beckman Coulter (1 mentions)
Unicel dxi 800 immunoassay system, by Beckman Coulter (1 mentions)
Bn 2 system, by Siemens (1 mentions)
U 2900 spectrophotometer, by Hitachi (1 mentions)
Cobalt chloride, by Merck Group (1 mentions)
20 protocols using lh 780 analyzer
1
Platelet-Lymphocyte Ratio and Neutrophil-Lymphocyte Ratio Analysis
2
Standardized Clinical Laboratory Testing
3
Comprehensive Blood Analysis for Coronary Angiography
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Peripheral venous blood samples were obtained from a forearm vein after at least 12 hours of overnight fasting before coronary angiography. Routine blood tests were performed as routine procedures in the Laboratory Department of the hospital. The red blood cell count, red cell distribution width, platelet count, and platelet distribution width were analyzed using a Beckman Coulter LH 780 analyzer (Miami, FL, USA). Triglycerides, total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, and fasting blood glucose were analyzed using a Siemens ADVIA 2400 analyzer (Tarrytown, NY, USA). The serum sEPCR level was measured by using a commercially available enzyme-linked immunosorbent assay (ELISA) kit (Lanji Biotech, Shanghai, China), in accordance with the manufacturer’s instructions.
4
Plasma Electrolytes and Cardiac Function
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The blood specimens, including plasma sodium, glucose, and BUN, were measured in all patients within 8 hours after admittance and analyzed by utilizing standard biochemical quantitative techniques, with the Beckman Coulter LH 780 Analyzer (Beckman Coulter Ireland Inc., Mervue, Galway, Ireland). The plasma osmolarity was measured using the following equation, which showed mean differences of <1 mOsmol/L compared to the direct measurement of the plasma osmolarity: 2 × sodium + 0.9 glucose + 0.93 × urea × 0.5.15 (link)Additionally, an echocardiographic evaluation of the left ventricular (LV) systolic function (ejection fraction) was performed within 24 hours after admission using a Vivid 7 imaging system (GE Vingmed Ultrasound AS, Horten, Norway) to investigate the patients. We utilized the Simpson’s rule to calculate the left ventricular ejection fraction (LVEF).
5
Platelet Indices Measurement Protocol
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Platelet indices were measured in blood samples drawn into collecting tubes containing tripotassium ethylenediaminetetraacetic acid (7.2 mg). All samples were analyzed by an automated blood counter (Beckman Coulter LH 780 Analyzer, Miami, FL, USA) within 1 h of collection.
6
Hematological Parameters Analysis Protocol
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Complete blood counts were performed by flow cytometry (Beckman Coulter LH 780 Analyzer; Beckman Coulter, Miami, FL, USA). Calibration of the analyzer was performed twice a day by the control blood samples with low and high parameters. Peripheral blood was obtained at the time of diagnosis. Venous blood samples were drawn into EDTA tubes for the evaluation of hematological parameters before treatment. Hematological parameters (leukocytes, neutrophils, lymphocytes, NLR, MPV, RDW, ESR, and CRP) were recorded. The NLR was calculated as follows: NLR = neutrophil count to lymphocyte count. CRP was measured by the turbidimetric method (Toshiba ACCUTE TBA-40FR; Toshiba Medical Systems, Tokyo, Japan). ESR was measured by spectrophotometric assay (Alifax test-1 THL, 950 nm; Alifax, Polverara, Italy).
7
Platelet-Lymphocyte Ratio Analysis
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Samples for PLR analysis were drawn on admission and analyzed within 1 h after sampling by a Beckman Coulter LH 780 Analyzer (Pasadena, California, USA). The blood samples were stored in ethylenediaminetetraacetic acid-containing tubes. PLR was defined as the absolute platelet count divided by the absolute lymphocyte count. NLR was defined as the absolute neutrophil count divided by the absolute lymphocyte count.
8
Blood Sample Collection and Analysis
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Blood samples were collected between 8:00 and 10:00 by venepuncture after a 12-hour fasting period. Serum and plasma were obtained after centrifugation (for 5 minutes at 1000 g). The CBC was measured in whole blood following standard laboratory techniques using the Beckman Coulter LH780 Analyzer (Beckman Coulter, Brea, CA). Routine coagulation tests including prothrombin time, activated partial thromboplastin time, and fibrinogen were assayed on a blood coagulation analyzer ACL TOP 700 (Beckman Coulter). Serum iron, serum ferritin, total iron binding capacity, serum vitamin B12, serum folate, sTfR, and other biochemical variables were determined by modular analyzers (Elecsys and Modular DP; Roche Diagnostics, Mannheim, Germany).
9
Serum Biomarker Measurement Protocol
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Serum PCT levels were measured with the electrochemiluminescence immunoassay method using a Roche Cobas E411 device at a reference range of 0.04–0.1 µg/L. Serum CRP levels were assayed via the nephelometric method using the Beckman Array 360 System at a reference range of 0–5 mg/L. The WBC count was determined using a hematological cell counter (LH 780 Analyzer, Beckman Coulter Inc., Miami, FL, USA) at a reference range of 4.2–10.6 × 103/µL.
10
Hematological Markers in Diagnosis
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All peripheral blood samples were obtained at the time of diagnosis.
Flow cytometry was used for complete blood count (Beckman Coulter LH 780 Analyzer; Beckman Coulter Inc. Miami, FL, USA). Hematological parameters (neutrophils, lymphocytes, NLR, MPV and RDW were recorded. The NLR was defined as follows: NLR=neutrophil count divided by lymphocyte count.
ACE was measured by spectrophotometric methods. ESR was also measured by spectrophotometric assay (Alifax test – 1 THL, 950 nm).
Flow cytometry was used for complete blood count (Beckman Coulter LH 780 Analyzer; Beckman Coulter Inc. Miami, FL, USA). Hematological parameters (neutrophils, lymphocytes, NLR, MPV and RDW were recorded. The NLR was defined as follows: NLR=neutrophil count divided by lymphocyte count.
ACE was measured by spectrophotometric methods. ESR was also measured by spectrophotometric assay (Alifax test – 1 THL, 950 nm).
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