For analysis of DC, the following monoclonal antibodies were used: anti-CD14, anti-HLA-DR, anti-CD80, anti-CD86, anti-CD83, and isotype controls (all from Miltenyi). 1 × 105 cells/tube were stained with fluorochrome conjugated mAbs (BD) and incubated for 30 min at 4 °C in the dark. All labeled cells were analyzed on a FACS Calibur (BD). Data analyses were conducted using CellQuest software (Becton Dickinson Company, Franklin Lakes, NJ, USA).
Anti cd83
Manufactured by Miltenyi Biotec
Sourced in United States
Anti-CD83 is a monoclonal antibody that binds to the CD83 cell surface molecule. CD83 is a member of the immunoglobulin superfamily and is expressed on mature dendritic cells. Anti-CD83 can be used for the identification and characterization of CD83-positive cells.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd80, by Miltenyi Biotec (3 mentions)
Anti cd86, by Miltenyi Biotec (3 mentions)
Anti hla dr, by Miltenyi Biotec (2 mentions)
Cellquest software, by BD (2 mentions)
Isotype controls, by Miltenyi Biotec (1 mentions)
Fluorochrome conjugated mabs, by BD (1 mentions)
Facscalibur, by BD (1 mentions)
Anti cd40, by BD (1 mentions)
Anti cd11c, by BD (1 mentions)
Anti cd209, by BD (1 mentions)
Anti cd1a, by BD (1 mentions)
Accuri c6, by BD (1 mentions)
Facscalibur system, by BD (1 mentions)
3 protocols using anti cd83
1
Characterization of Dendritic Cells
2
Phenotypic Characterization of Monocyte-Derived Dendritic Cells
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Before and after differentiation, the cells were collected, washed and resuspended in Dulbecco’s phosphate-buffered saline (D-PBS, HyClone). To analyze surface antigens, anti-CD1a (BD Biosciences, San Jose, CA), anti-CD11c (BD Biosciences), anti-CD14 (Miltenyi Biotec Gmbh), anti-CD40 (BD Biosciences), anti-CD80 (Miltenyi Biotec Gmbh), anti-CD83 (Miltenyi Biotec Gmbh), anti-CD86 (Miltenyi Biotec Gmbh) and anti-CD209 (BD Biosciences) fluorescence monoclonal antibodies were used. Matched labeled isotypes were used as controls. The labeled cells were analyzed using flow cytometry (Accuri C6, BD Biosciences). The screening criterion of monocyte-derived DCs was defined as CD40+CD209+ cells in this study [55 (link), 56 (link)].
3
Flow Cytometric Analysis of DC Phenotype
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For the analysis of surface DC phenotype using flow cytometry, the following monoclonal antibodies (mAb) were used: FITC-labeled anti-CD14 and anti-HLA I (both from Invitrogen, Camarillo, CA), Alexa Fluor 488-labeled anti-CCR7 (Biolegend, CA, USA), PE-labeled anti-CD40, anti-CD80, anti-CD83, anti-CD86, and anti-HLA DR (Miltenyi Biotec, Bergisch Gladbach, Germany).
The DCs, which were differentiated in Cellgenix® DC GMP medium (Cellgenix GmbH, Freiburg, Germany), were harvested and collected through centrifugation. Prior to cell staining, the cells were washed two times in DPBS in all instances. The appropriate antibody was added, and the cells were incubated in the dark for 15 min. Subsequently, the cells were washed twice and suspended in 2% paraformaldehyde (PFA). Analysis of the samples was performed using a FACSCalibur system (Becton Dickinson, Inc.), and the acquired data were analyzed using the CellQuest software (BD biosciences).
The DCs, which were differentiated in Cellgenix® DC GMP medium (Cellgenix GmbH, Freiburg, Germany), were harvested and collected through centrifugation. Prior to cell staining, the cells were washed two times in DPBS in all instances. The appropriate antibody was added, and the cells were incubated in the dark for 15 min. Subsequently, the cells were washed twice and suspended in 2% paraformaldehyde (PFA). Analysis of the samples was performed using a FACSCalibur system (Becton Dickinson, Inc.), and the acquired data were analyzed using the CellQuest software (BD biosciences).
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