Egm mv2

Human PAECs and human SMCs (PASMCs) were purchased from PromoCell. Human microvascular ECs of the dermis and the lings (MVECs-D and MVECs-L) and human coronary artery ECs (CAEC) were purchased from Clonetics. PAECs and CAECs were cultured in HuMedia-EG2 (Kurabo), and hMVECs-L and hMVECs-D were cultured in EGM-MV2 (Lonza). When the cells became subconfluent (~90%), RNA was extracted from cultured ECs using TRIzol (Invitrogen). RNAs for 5-donor pooled human lungs, heart, kidneys, and liver were obtained from BioChain Institute. Labeled cRNA was prepared using 1 vial of the mixed RNAs for each sample according to the One-Color Microarray-Based Gene Expression Analysis ver. 6.5 protocol, and 600 ng of cRNAs was used for hybridization with microarray chips (SurePrint G3 Human GE; G4851B) for 17 h. After washing, signals were scanned using an Agilent DNA microarray scanner, and the data were analyzed using Feature Extraction ver. 10.7.1.1 (Agilent Technologies).

Human lung microvascular endothelial cells (hMVECs) were cultured as previously described (12 (link)). In brief, hMVECs were purchased from Lonza (Basel, Switzerland) and cultured in EGM MV2 with microvascular endothelial cell supplementary kit C-22121 (Lonza, Basel, Switzerland) on cell culture ware coated with 1% gelatin until they reached 90% confluency. Cells were used from passage 4 to 7.

Human PAECs and human SMCs (PASMCs) were purchased from PromoCell. hMVECs-L and hMVECs-D were purchased from Clonetics. PAECs were cultured in HuMedia-EG2 (Kurabo), and PASMCs were cultured in Smooth Muscle Cell Growth Medium 2 (PromoCell). hMVECs-L and hMVECs-D were cultured in EGM-MV2 (Lonza).