Log In Sign up
The largest database of trusted experimental protocols

Small airway epithelial cell growth medium

Manufactured by Lonza
Visit Supplier

Small airway epithelial cell growth medium is a cell culture medium designed to support the growth and maintenance of human small airway epithelial cells in vitro. It provides the necessary nutrients and growth factors required for the proliferation and differentiation of these specialized cells. The medium composition is optimized to maintain the phenotypic characteristics of the cells.

Automatically generated - may contain errors

Lab products found in correlation

Compound 3, by Cayman Chemical (1 mentions) Poly 1 c, by Merck Group (1 mentions) Poly 1 c hmw, by InvivoGen (1 mentions) Hsaec, by Lonza (1 mentions) Nhbes, by Lonza (1 mentions) Saecs, by Lonza (1 mentions) Bronchial epithelial cell growth medium, by Lonza (1 mentions)

6 protocols using small airway epithelial cell growth medium

1

Evaluating SAEC and COPD-SAEC Response to PG and Gly

Check if the same lab product or an alternative is used in the 5 most similar protocols
Commercially available primary human SAECs and COPD-SAECs were used in this study. SAECs and COPD-SAECs were purchased from LONZA (Basel, Switzerland). For SAECs, cells from healthy and non-smoker donors were selected. Both sets of cells were cultured in small airway epithelial cell growth medium (LONZA) in accordance with the manufacturer’s instructions. Cells from passages 2–4 were used for the assays. Three batches of SAECs and COPD-SAECs were evaluated on two separate occasions in each experiment. PG (FUJIFILM WAKO Pure Chemical Corporation, Osaka, Japan) or Gly (FUJIFILM WAKO Pure Chemical Corporation) were added into the culture medium at concentrations of 0–4%.
Komura M., Sato T., Yoshikawa H., Nitta N.A., Suzuki Y., Koike K., Kodama Y., Seyama K, & Takahashi K. (2022). Propylene glycol, a component of electronic cigarette liquid, damages epithelial cells in human small airways. Respiratory Research, 23, 216.
+ Open protocol
+ Expand
2

Human Small Airway Epithelial Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols
The human telomerase reverse transcriptase (hTERT) immortalized human small airway epithelial (SAE) cells were previously generated [24 (link)]. Cells were maintained in serum-free Small Airway Epithelial Cell Growth Medium supplemented with various growth factors supplied by the manufacturer (Lonza). Wild-type and DRP1 knockout HCT116 cell line were obtained from the National Institute of Neurological Diseases and Stroke, NIH (Bethesda, MD) through Dr. C. Wang [25 (link)]. Cells were maintained in McCoy 5A’s medium (Life Technologies) supplemented with glutamine (2 mmol/L) and nonessential amino acid (1X). Cultured cells were maintained at 37°C in a humidified 5% CO2 incubator.
Wu J., Zhang B., Wuu Y.R., Davidson M.M, & Hei T.K. (2017). Targeted Cytoplasmic Irradiation and Autophagy. Mutation research, 806, 88-97.
+ Open protocol
+ Expand
3

Culturing and Treating Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
HeLa cervical cancer and primary human foreskin fibroblasts (HFF) cells were cultured in DMEM supplemented with 10% FBS and penicillin/streptomycin. MCF7 breast cancer cells were cultured in RPMI supplemented with 10% FBS and penicillin/streptomycin. HSAEC1-KT epithelial cells (ATCC) were cultured in Small Airway Epithelial Cell Growth Medium (Lonza) supplemented with the contents of the SAGM SingleQuot Kit. Cell lines were maintained at 37 °C in an atmosphere of 5% CO2 and routinely sub-cultured by Trypsin. Immortal cell lines were used at passage numbers 30 or lower and checked to be mycoplasma-free on a monthly basis. Stock solutions of 1 and 2 (20 mM) or CHIR-124 (10 mM) were prepared in DMSO before dilution in cell medium (final DMSO concentration = 0.5%). Cells treated with 1 or 2 were shielded from light to minimise phototoxicity. Cisplatin (2 mM) and mitoxantrone (5 mM) stock solutions were prepared in 100% PBS and each agent applied in DMSO-free media.
Gill M.R., Harun S.N., Halder S., Boghozian R.A., Ramadan K., Ahmad H, & Vallis K.A. (2016). A ruthenium polypyridyl intercalator stalls DNA replication forks, radiosensitizes human cancer cells and is enhanced by Chk1 inhibition. Scientific Reports, 6, 31973.
+ Open protocol
+ Expand
4

Immortalized Human Small Airway Epithelial Cell Culture

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Immortalized human small airway epithelial cells (hSAECs) were previously described.9 (link) hSAECs were grown in small airway epithelial cell growth medium (Lonza, Walkersville, MD) in a humidified atmosphere of 5% CO2. Poly(I:C) was obtained from Sigma (St. Louis, MO) and used at 10 μg/mL in cell culture. Compound 1 was purchased from Tocris, and compound 3 was purchased from Cayman Chemical (Ann Arbor, Michigan). Compounds were solubilized in DMSO and added at the indicated concentrations.
Liu Z., Li Y., Chen H., Lai H.T., Wang P., Wu S.Y., Wold E.A., Leonard P.G., Joseph S., Hu H., Chiang C.M., Brasier A.R., Tian B, & Zhou J. (2022). Discovery, X‑ray Crystallography, and Anti-inflammatory Activity of Bromodomain-containing Protein 4 (BRD4) BD1 Inhibitors Targeting a Distinct New Binding Site. Journal of medicinal chemistry, 65(3), 2388-2408.
+ Open protocol
+ Expand
5

NF-κB Activation in Primary Human Small Airway Epithelial Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Two strains of primary human small airway epithelial cells (hSAEC) from different donors were purchased from Lonza Inc., (Allendale, NJ) and cultured in small airway epithelial cell growth medium (Lonza) supplemented as recommended by the supplier, and used for experiments at early passage (16 (link)). For the preparation of SAECs containing an NF-κB-luciferase reporter, hSAECs were infected with commercially available ready-to-transduce lentiviral particles that express the firefly luciferase gene under the control of a minimal CMV promoter and tandem repeats of the NF-κB transcriptional response element (SA Biosciences; Valencia CA). Typically, hSAECs were pre-incubated for 24 hours in basal medium (without supplements) to minimize the effect of medium components on inducing inflammatory signaling. Cells were then pretreated with RvD1 for 30 minutes, followed by 5μ/ml of high molecular weight (HMW) poly(I:C) (Invivogen, San Diego, CA) for the indicated time points.
Hsiao H.M., Thatcher T.H., Levy E.P., Fulton R.A., Owens K.M., Phipps R.P, & Sime P.J. (2014). Resolvin D1 Attenuates Poly(I:C)-Induced Inflammatory Signaling in Human Airway Epithelial Cells via TAK1. Journal of immunology (Baltimore, Md. : 1950), 193(10), 4980-4987.
+ Open protocol
+ Expand
6

Comparative Analysis of Airway Cell Responses

Check if the same lab product or an alternative is used in the 5 most similar protocols
SAECs and NHBEs were purchased from Lonza. Three batches of each cell type, each originally isolated from three different healthy never-smoker donors, were used. SAECs were cultured with small airway epithelial cell growth medium (Lonza), and NHBEs were cultured with bronchial epithelial cell growth medium (Lonza), according to the manufacturer’s instructions (SAECs and NHBEs were seeded at 2500 and 3500 cells/cm2, respectively, in 25 cm2 plastic flasks). Both cell types were used at passages three to six and assayed at the same passages for direct comparisons. After reaching approximately 80% confluence, cells were treated with or without 2.5% CSE until assayed.
Baskoro H., Sato T., Karasutani K., Suzuki Y., Mitsui A., Arano N., Nurwidya F., Kato M., Takahashi F., Kodama Y., Seyama K, & Takahashi K. (2018). Regional heterogeneity in response of airway epithelial cells to cigarette smoke. BMC Pulmonary Medicine, 18, 148.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!