Fast total rna extraction kit

Manufactured by Fastagen

The Fast total RNA extraction kit is a laboratory product designed to efficiently extract total RNA from a variety of biological samples. It provides a streamlined process to isolate high-quality RNA for downstream applications.

Automatically generated - may contain errors

Lab products found in correlation

Lightcycler 480 2, by Roche (2 mentions) Qpcr supermix, by Transgene (1 mentions) Reverse transcription kit, by Vazyme (1 mentions) Sybr green qpcr mix, by Yeasen (1 mentions)

2 protocols using fast total rna extraction kit

Cytokine-Induced Gene Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

After being treated with cytokine for 24h, the cells were digested with trypsin and collected in 1.5 mL tubes, then the total cellular RNA was extracted using Fast total RNA extraction kit (FASTAGEN, Shanghai, China) and reverse-transcribed. Quantitative Real-time PCR (qRT-PCR) was performed using the qPCR SuperMix (TransGen) on Roche LightCycler480II (Basel, Switzerland). The relative gene expression levels were calculated by the comparative threshold cycle method (2^–ΔΔCT). The primer sequences are shown in Supplementary Materials (Table S1).

Zhang Y., Zeng H., Hu Y., Jiang L., Fu C., Zhang L., Zhang F., Zhang X., Zhu L., Huang J., Chen J, & Zeng Q. (2022). Establishment and validation of evaluation models for post-inflammatory pigmentation abnormalities. Frontiers in Immunology, 13, 991594.

+ Open protocol

+ Expand

Comprehensive qPCR Analysis of Pigmentation and Signaling Genes

Check if the same lab product or an alternative is used in the 5 most similar protocols

The total cellular RNA was extracted using Fast total RNA extraction kit (FASTAGEN, Shanghai, China), and reverse-transcribed into cDNA using a reverse transcription kit (#R223-01, Vazyme), as per manufacturer’s instructions. The SYBR green qPCR Mix (#11184ES08, YEASEN) was used, and the reaction was performed on a real-time PCR instrument (Roche Light Cycler 480II, Basel, Switzerland). GAPDH was used as the internal control. The relative expression levels of MITF, TYR, TYRP1, DCT, RAB27A, MYO5A, VEGF, PTGS2, FGF2, ET-1, WNT3, WNT7A, TRPV1, TRPV2, TRPV3, and TRPV4, were calculated according to the ΔCt formula and normalized against GAPDH housekeeping gene. The primer sequences are listed in Table S1.

Zhang L., Zeng H., Jiang L., Fu C., Zhang Y., Hu Y., Zhang X., Zhu L., Zhang F., Huang J., Chen J, & Zeng Q. (2023). Heat promotes melanogenesis by increasing the paracrine effects in keratinocytes via the TRPV3/Ca2+/Hh signaling pathway. iScience, 26(5), 106749.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!