Olaparib s1060

Manufactured by Selleck Chemicals

Sourced in Germany, United States

Olaparib (S1060) is a lab equipment product. It is a PARP inhibitor.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Olaparib (453 citations) S1060 (7 citations)

5 protocols using olaparib s1060

Detecting DNA Damage and Repair

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mitomycin C (MMC, M0503), diepoxybutane (DEB, ref. 202533-16) 5-bromo-2-deoxyuridine (BrdU, B5002) and Hoechst 33258 (B2883) and IGEPAL (I8896) were from Sigma (Madrid, Spain). Ethidium monoazide bromide (EMA, e1374) and Sytox Green (S7020) were from Thermofischer Scientific (Barcelona, Spain). PARP inhibitor Olaparib (S1060) was from Selleckchem (Munich, Germany). Propidium iodide (P3566) and RNAse (12091021) were from Invitrogen (Barcelona, Spain). Colcemid was from Gibco (Barcelona, Spain). Antibodies used included: mouse monoclonal to CDK5RAP3 (ab57817), rabbit polyclonal to BRCA2 (ab123491), mouse monoclonal to RPA32 (ab2175), rabbit polyclonal to actin (ab1801), rabbit polyclonal to GAPDH (ab9485) and mouse monoclonal to vinculin (ab18058) were from Abcam (Cambridge, UK). Rabbit polyclonal to RAD51 (SC8349) was from Santa Cruz biotechnology (Heidelberg, Germany).

Minguillón J., Ramírez M.J., Rovirosa L., Bustamante-Madrid P., Camps-Fajol C., Ruiz de Garibay G., Shimelis H., Montanuy H., Pujol R., Hernandez G., Bogliolo M., Castillo P., Soucy P., Martrat G., Gómez A., Cuadras D., García M.J., Gayarre J., Lázaro C., Benítez J., Couch F.J., Pujana M.A, & Surrallés J. (2022). CDK5RAP3, a New BRCA2 Partner That Regulates DNA Repair, Is Associated with Breast Cancer Survival. Cancers, 14(2), 353.

+ Open protocol

+ Expand

Antibodies for PARP Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The antibodies used in this study were the rabbit polyclonals anti-XRCC1 (Millipore; ABC738), anti-PARP2 (Active Motif; 39743), anti-PARP3 (4699; a kind gift from F. Dantzer), anti-PNKP (SK3195) (32 (link)), anti-poly (ADP-ribose) (Trevigen; 4336), rabbit Fc-fused anti-pan-ADP-ribose binding reagent (Millipore; MABE1016) and the mouse monoclonals anti-PARP1 (Serotec; MCA1522G), anti-poly (ADP-ribose) 10H (Enzo; ALX-804-2), anti-nucleophosmin (B23) (Invitrogen; 325200) and anti-actin (Sigma; A4700). The secondary antibodies employed for Western blotting were HRP-conjugated goat anti-rabbit (Bio-Rad; 170-6515) and goat anti-mouse (Bio-Rad; 170-6516) and for indirect immunofluorescence were goat anti-mouse or anti-rabbit Alexa 488 (Invitrogen; A11001 and A31628), goat anti-mouse Alexa 555 (Invitrogen; A21422) and donkey anti-mouse Alexa 647 (Invitrogen; A39571). 8.8 M H₂O₂ was obtained from Fischer Scientific. Olaparib (S1060) was purchased from Selleckchem and KU0058948 hydrochloride from Axon. Both PARP inhibitors were dissolved in dimethyl sulfoxide (Sigma) to a working concentration of 10 mM.

Hanzlikova H., Gittens W., Krejcikova K., Zeng Z, & Caldecott K.W. (2016). Overlapping roles for PARP1 and PARP2 in the recruitment of endogenous XRCC1 and PNKP into oxidized chromatin. Nucleic Acids Research, 45(5), 2546-2557.

+ Open protocol

+ Expand

Protein Expression and Apoptosis Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies for UBE2S (14115–1-AP) and β-actin (66009–1-Ig) were purchased from Proteintech (Wuhan, China). Antibodies for PARP (9532) and caspase-3 (9662) were obtained from Cell Signaling Technology (Danvers, MA, USA). Antibodies for c-Myc (ab32072), β-catenin (ab32572), and cyclin D1 (ab16663) were acquired from Abcam (Cambridge, UK). XAV-939 (S1180) and Olaparib (S1060) were obtained from Selleck Chemicals (Houston, TX, USA). Tosyl Arginine Methyl Ester (TAME, 172104) was purchased from Sigma-Aldrich (St. Louis, MO, USA).

Hu W., Li M., Chen Y, & Gu X. (2021). UBE2S promotes the progression and Olaparib resistance of ovarian cancer through Wnt/β-catenin signaling pathway. Journal of Ovarian Research, 14, 121.

+ Open protocol

+ Expand

ASPM Localization and DNA Damage Response

Check if the same lab product or an alternative is used in the 5 most similar protocols

All ASPM fragments were sub-cloned into a pEGFP-C1 vector. Olaparib (S1060), Etoposide (S1225), MG132 (S2619), Cycloheximide (S7418), NU6027 (S7114), KU55933 (S1092) and NU7026 (S2893) were purchased from Selleck. Rabbit anti-ASPM(BL1615), anti-RPA32 (A300-244A), anti-pKAP1 S824 (A700-013), anti-KAP1 (A700-014), anti-BRCA1 (A301-377A), anti-pRPA32 S33 (A300-246A) and anti-ATM (A300-299A) antibodies were purchased from Bethyl. Rabbit anti-pATM S1981 (ab81292) and anti-HERC2 (ab85832) were purchased from Abcam. Anti-BRCA1 (sc-6954) and anti-CtIP (sc-271339) was purchased from Santa Cruz, anti-BrdU (347580) from BD Biosciences, and anti-FLAG M2 (F1804), anti-FLAG® M2 Affinity Gel (A2220) and anti-β-actin (A5441) from Sigma.

Xu S., Wu X., Wang P., Cao S.L., Peng B, & Xu X. (2021). ASPM promotes homologous recombination-mediated DNA repair by safeguarding BRCA1 stability. iScience, 24(6), 102534.

+ Open protocol

+ Expand

Antibody Sources and Research Chemicals for Cell Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols

The sources of antibodies against the following proteins were as follows: ECT2 (07-1364, for Western blotting [WB], ChIP, and IF) and γH2AX (05-636, for IF and WB) were purchased from Millipore; poly(ADP-ribose) (ALX-840-220-R100, for IF) was purchased from ENZO; HA (sc-805, for WB) and PARP1 (sc-7150 for WB) were purchased from Santa Cruz Biotechnology; β-actin (AC026, for WB) was purchased from ABclonal; FLAG (F1804, for IP and WB) was purchased from Sigma; RAD51 (ab63801, for IF and WB) and RAD50 (ab89, for WB) were purchased from Abcam; BRCA1 (22362-1-AP, for IF and WB), KU70 (10723-1-AP, for WB), and KU80 (16389-1-AP, for WB) were purchased from Proteintech; H3 (YM3038, for WB), H2AX (YT2155, for WB), and LaminA/C (YT2521, for WB) were purchased from ImmunoWay. Anti-FLAG M2 affinity gel (A2220), 3× FLAG peptide (F4799), puromycin (P8833), 4-OHT (H6278), KU55933 (SML1109), and NU7026 (N1537) were purchased from Sigma. Olaparib (S1060) was purchased from Selleck. MNNG (70-25-7) was purchased from Tokyo Chemical Industry.

Cao C., Han P., Liu L., Tang Y., Tian S., Zhang K., Shi L., Liu Z., Zhuo D., Ge W, & Gong W. (2021). Epithelial cell transforming factor ECT2 is an important regulator of DNA double-strand break repair and genome stability. The Journal of Biological Chemistry, 297(3), 101036.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!