Ethylene diaminetetraacetic acid disodium salt dihydrate edta

Manufactured by Merck Group

Sourced in Germany, United States, United Kingdom

Ethylene-diaminetetraacetic acid disodium salt dihydrate (EDTA) is a chemical compound used as a chelating agent. It is a white, crystalline powder that is soluble in water. EDTA's primary function is to form stable complexes with metal ions, which can be useful in various applications.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Disodium EDTA (28 citations) EDTA disodium salt (14 citations) EDTA) disodium salt dihydrate (5 citations) Disodium salt dihydrate (5 citations)

5 protocols using ethylene diaminetetraacetic acid disodium salt dihydrate edta

HPLC Analysis of Glyphosate and AMPA

Check if the same lab product or an alternative is used in the 5 most similar protocols

High pressure liquid chromatography (HPLC) grade acetonitrile and pH
buffers (4.0, 7.0 and 10.0) were purchased from VWR (Atlanta, GA).
Aminomethylphosphonic acid (AMPA) analytical standard was purchased from Acros
Organics (Geel, Belgium). Glyphosate (PMG) analytical standards,
ethylene-diaminetetraacetic acid disodium salt dihydrate (EDTA) and acetic acid
were purchased from Sigma-Aldrich (Saint Louis, MO). HPLC grade acetone and
water + 0.1% formic acid were purchased from Fisher Scientific (Hampton, NH).
Activated carbon (AC) derived from coconut shell (mesh size: 100 – 325;
iodine number: 1100 mg/g; bulk density: 30 – 33 lbs/ft.³) was
obtained from the General Carbon Corporation (Paterson, NJ). Calcium
montmorillonite (CM) was obtained from Engelhard Corp. (Cleveland, OH). CM has
an average total surface area of 850 m²/g, an external surface area
of approximately 70 m^2/g, and a cation exchange capacity of 97
cmol/kg (Grant and Phillips, 1998 (link)). CM
has a general formula of
(Ca)_0.3(Al,Mg)₂Si₄O₁₀(OH)₂•nH₂O
(Marroquin-Cardona et al., 2011 (link)).
Deionized water (18.2 MΩ) was generated in the laboratory using an
Elga™ automated filtration system (Woodridge, IL) and was used in all
experiments.

Hearon S.E., Wang M., McDonald T.J, & Phillips T.D. (2020). Decreased bioavailability of aminomethylphosphonic acid (AMPA) in genetically modified corn with activated carbon or calcium montmorillonite clay inclusion in soil. Journal of environmental sciences (China), 100, 131-143.

+ Open protocol

+ Expand

Development of DNA Aptamer Library

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sodium chloride, magnesium chloride, potassium chloride, calcium chloride, 2-amino-2-(hydroxymethyl)-1,3-propanediol (Trizma base), hydrochloric acid, 2,5-dimethyl-4-hydroxy-3(2H)-furanone (furaneol), 5-Ethyl-3-hydroxy-4-methyl-2(5H)-furanone (maple furanone), 4,5-Dimethyl-3-hydroxy-2,5-dihydrofuran-2-one (sotolon), 5-Ethyl-4-hydroxy-2-methyl-3(2H)-furanone (homofuraneol), 2-Methoxyphenol (guaiacol), and ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA) were purchased from Sigma-Aldrich (Munich, Germany). Taq DNA polymerase, dNTPs solution, TAE and TBE electrophoresis buffers, DNA extraction kits, DNA cloning kit, Exonuclease I, and the Escherichia coli DH5α strain were purchased from Thermo Fisher Scientific (Waltham, MA, USA). SYBR Gold dye was purchased from Invitrogen (Thermo Fisher Scientific). SYBR Green I dye was obtained from Lumiprobe Ltd., (Moscow Russia). Streptavidin-coated magnetic beads were supplied by New England Biolabs (Ipswich, MA, USA).
The single-stranded DNA library B1_bank was synthesized by DNA Synthesis Ltd., Moscow, Russia. All primers for DNA amplification were ordered from the DNA Synthesis Ltd., Moscow, Russia. The B1_cap was either purchased from DNA Synthesis Ltd., Moscow, Russia or from Microsynth AG (Balgach, Switzerland). Candidate aptamer sequences were synthesized by Evrogen Ltd. (Moscow, Russia).

Komarova N., Andrianova M., Glukhov S, & Kuznetsov A. (2018). Selection, Characterization, and Application of ssDNA Aptamer against Furaneol. Molecules, 23(12), 3159.

+ Open protocol

+ Expand

Antioxidant Activity Evaluation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fipronil (PESTANAL, analytical standard; product code: 46451; empirical formula: C₁₂H₄Cl₂F₆N₄OS; molecular weight: 437.15 g/mol; CAS number: 120068-37-3; ≥98.8% purity), quercetin (product code: 337951; molecular weight: 302.21 g/mol; CAS number: 849061-97-8; ≥95% purity), and curcumin [curcumin from Curcuma longa (Turmeric), powder; product code: C1386; molecular weight: 368.38 g/mol; CAS number. 458-37-7; assay ≥65% (HPLC)] were purchased from Sigma Aldrich (Germany) and used.
DL-Dithiothreitol, 5,5’-Dithiobis (2-nitrobenzoic acid) (DTNB), ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA), phenylmethylsulfonyl fluoride, potassium chloride, hydrogen peroxide solution, Triton X-100, Trizma base, pyrogallol, L-glutathion reduced, β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate (β-NADPH), glutathion reductase, ammonium sulfate, trichloroacetic acid, 1-chloro-2,4-dinitrobenzene (CDNB), butylated hydroxytoluene, 2-thiobarbituric acid (TBA), bovine serum albumin, and disodium hydrogen phosphate were obtained from Sigma Aldrich. Hydrochloric acid, Folin–Ciocalteu’s phenol reagent, sodium hydroxide, and potassium dihydrogen phosphate were purchased from Merck (Germany).

Uzunhisarcikli M., Apaydin F.G., Bas H, & Kalender Y. (2023). The ameliorative effects of quercetin and curcumin against subacute nephrotoxicity of fipronil induced in Wistar rats. Toxicology Research, 12(3), 493-502.

+ Open protocol

+ Expand

Robust RNA Extraction Using CTAB-SSTE Method

Check if the same lab product or an alternative is used in the 5 most similar protocols

The hexadecyltrimethylammonium bromide (CTAB; Cat. no. 52365; Sigma, USA) protocol of Chang et al. [30 (link)] was tried to extract RNA. The extraction using CTAB buffer was followed by SSTE buffer [30 (link)]. All the solutions were prepared with nuclease-free water. The composition of the CTAB extraction buffer was: 2% (w/v) CTAB, 2% (w/v) polyvinylpyrrolidone K-40 (PVP, Cat. no. PV40; Sigma, USA), 100 mM Tris-HCl (pH 8.0; Trizma base, Cat. no. 1503; Sigma, USA), 25 mM ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA; Cat. no. EA023, Sigma, USA), 2.0 M sodium chloride (NaCl; Cat. no. 1103414; Sigma, USA), 0.5 g/1 spermidine (free acid, S2626-1G, Sigma, USA), 5% (v/v) β-mercaptoethanol (added just before use, Cat. no. 63689; Sigma, USA). SSTE buffer consisted of 1.0 M NaCl, 0.5% (w/v) sodium dodecyl sulfate (SDS, Cat. no. L3771; Sigma, USA), and 10 mM Tris-HC1 (pH 8.0).

Sasi S., Krishnan S., Kodackattumannil P., Shamisi A.A., Aldarmaki M., Lekshmi G., Kottackal M, & Amiri K.M. (2023). DNA-free high-quality RNA extraction from 39 difficult-to-extract plant species (representing seasonal tissues and tissue types) of 32 families, and its validation for downstream molecular applications. Plant Methods, 19, 84.

+ Open protocol

+ Expand

Antibody Purification and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protein A-purified rabbit polyclonal anti-E.coli IgG class antibodies were obtained from GenScript (UK). Protein A-purified rabbit polyclonal anti-myoglobin IgG class antibodies were acquired from GenScript (UK). Protein G-purified sheep anti-digoxin IgG class antibodies were obtained from Therapeutic Antibodies Ltd. (UK). Myoglobin from equine heart, PBS tablets, sodium chloride, sodium acetate trihydrate, acetic acid, hydrochloric acid, and ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA) were purchased from Sigma (UK). 2-mercaptoethylamine was acquired from Alfa Aesar (UK). Tris(2carboxyethyl)phosphine hydrochloride was obtained from Thermo Scientific (UK). Amicon Ultra-0.5 mL centrifugal filters (50KDa MWCO) were acquired from Merck Millipore (UK).
All chemicals were of analytical grade or better and the solutions were prepared in UHQ water. G:BOX equipped with GeneSys image acquisition software and analyzed using GeneTools analysis software (SynGene, UK).

Makaraviciute A., Jackson C.D., Millner P.A, & Ramanaviciene A. (2016). Considerations in producing preferentially reduced half-antibody fragments. Journal of immunological methods, 429.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!