Zorbax bonus rp

Manufactured by Agilent Technologies

Sourced in France, Canada

The Zorbax Bonus RP is a reversed-phase high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a wide range of compounds. It features a silica-based stationary phase with a proprietary bonding technology that provides enhanced selectivity and efficiency.

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Lab products found in correlation

Oasis hlb, by Waters Corporation (1 mentions) Strata x, by Phenomenex (1 mentions) Oasis prime hlb, by Waters Corporation (1 mentions) Kinetex polar c18, by Phenomenex (1 mentions) Kinetex xb c18, by Phenomenex (1 mentions) Luna omega polar, by Phenomenex (1 mentions) Zorbax eclipse plus c18, by Agilent Technologies (1 mentions) Openlab cds chemstation edition, by Agilent Technologies (1 mentions) Hplc system, by Agilent Technologies (1 mentions) Zorbax sb c18, by Agilent Technologies (1 mentions)

Close spelling variants of this product

ZORBAX Bonus-RP C18 column Zorbax Bonus RP column

4 protocols using zorbax bonus rp

Quantifying Paralytic Shellfish Toxins

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To extract the PST, 5 mL of 0.1 N hydrochloric acid were added, and the samples were mixed with a high-speed homogenizer (15,000 rpm) for 2 min. The pH was adjusted between 2.0 and 4.0, then the samples were centrifuged at 4200× g for 10 min at 4 °C. The supernatants were filtered on 10-kDa polyethersulfone (PES) filters, and the toxin content was analyzed using the liquid chromatography with fluorescence detection (LC/FD) PSP toxin analyses method of Van de Riet [69 (link)]. The toxins GTX, dc-GTX, dc-STX and STX were separated using a reverse chromatography column (Zorbax Bonus RP, 3.5 μM, 4.6 mm × 150 mm, Agilent Technologies, Massy, France) with a flow rate of 0.8 mL·min⁻¹. The eluent pH and/or column temperature were optimized to separate dc-GTX3/GTX5/dc-GTX-2 and C1/C2. The toxin concentrations were determined using certified standards provided by CNRC (Halifax, NS, Canada).

Boullot F., Castrec J., Bidault A., Dantas N., Payton L., Perrigault M., Tran D., Amzil Z., Boudry P., Soudant P., Hégaret H, & Fabioux C. (2017). Molecular Characterization of Voltage-Gated Sodium Channels and Their Relations with Paralytic Shellfish Toxin Bioaccumulation in the Pacific Oyster Crassostrea gigas. Marine Drugs, 15(1), 21.

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Paralytic Shellfish Toxin Analysis

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Alaska DEC EHL received a single unspecified unshucked clam on 2 June 2016. The shucked weight was ~11 g. High-pressure liquid chromatography with post-column oxidation (HPLC-PCOX, AOAC Method 2011.02) testing procedure was used to analyze the shellfish sample: homogenized shellfish samples were mixed with dilute hydrochloric acid and heated in a boiling water bath for 5 min. An aliquot of the supernatant was deproteinated with trichloroacetic acid, and the pH was adjusted to ~3. A portion of filtered extract was chromatographed on a C18 silica column (Zorbax Bonus-RP, Agilent, Santa Clara, CA) under gradient conditions using a heptane sulfonic acid/phosphoric acid buffer system for the analysis of GTX and STXs. The extract was also chromatographed on a C8 silica column (BetaBasic 8, ThermoScientific, Waltham, MA) using an isocratic tetrabutylammonium phosphate buffer system to determine the N-sulfocarbamoyl GTX. The toxins were derivatized by PCOX at 85°C with a phosphoric acid and periodic acid buffer solution. This oxidized effluent was acidified using nitric acid, and the derivatives were detected by fluorescence (excitation: 330 nm; emission: 390 nm). Calibrators were prepared using certified reference materials (CRM, National Research Council Canada).

Coleman R.M., Ojeda-Torres G., Bragg W., Fearey D., McKinney P., Castrodale L., Verbrugge D., Stryker K., DeHart E., Cooper M., Hamelin E., Thomas J, & Johnson R.C. (2018). Saxitoxin Exposure Confirmed by Human Urine and Food Analysis. Journal of analytical toxicology, 42(7), e61-e64.

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Comprehensive Analytical Method Development

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The following materials were investigated during method development. LC column types: Zorbax Bonus RP, Zorbax Eclipse Plus C18 from Agilent (Santa Clara, CA); YMC PACK ODS-AQ (Kyoto, Japan); Aeris Peptide, Luna Omega Polar, Kinetex XB-C18, Kinetex Polar C18 from Phenomenex (Torrance, CA). Solid-phase extraction products: Oasis^® HLB and Oasis HLB PRiME^® (Waters Corp., Milford, MA), Captiva^® EMR-Lipid (Agilent), Strata-x (Phenomenex). Syringe filters: Different membranes for 13 mm, 2 μm syringe filters including Nylon, PTFE, polyethersulfone (PES), polyvinylidene fluoride (PVDF) (Pall Corp., Port Washington, NY). Molecular weight cut-off centrifugal filter units: Microcon 3 kDa, 10 kDa, 30 kDa (Millipore Sigma, Burlington, MA).

Wu I.L., Turnipseed S.B., Andersen W.C, & Madson M.R. (2020). Analysis of peptide antibiotic residues in milk using liquid chromatography-high resolution mass spectrometry (LC-HRMS). Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 37(8), 1264-1278.

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HPLC Retention Measurements Protocol

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Retention measurements were made using an Agilent HPLC system (Waldbronn, Germany). The system included a binary pump (G4220A) with Jet Weaver V35 Mixer (G4220-68135), autosampler (G7167A), thermostat- ted column compartment (G1316C), and diode-array detector (G4212A) equipped with a Max-Light Cartridge Cell (G4212-60038, 10 mm path length). The system was controlled using Agilent OpenLAB CDS Chemstation Edition (Rev. C.01.10). The injection volume for each analysis was 0.15 μL. Two columns were used in this work: 1) Agilent Zorbax SB-C18 (5 mm x 2.1 mm i.d., 1.8 μm); 2) Agilent Zorbax Bonus RP (5 mm × 2.1 mm i.d., 1.8 μm) (see Table S1).
The flow rate for all measurements was 1.0 mL/min, and the temperature was 40 • C. Mobile phases were "machinemixed" by the binary pump. A minimum of five mobile phases were used for each compound, where the compositions were chosen such that all retention factors were between 1 and 50, but roughly evenly spaced in that range. Five replicate retention measurements were made at each composition, and the means of these values were used as described in Section 3.1.

Brau T., Pirok B., Rutan S, & Stoll D. (2022). Accuracy of retention model parameters obtained from retention data in liquid chromatography. Journal of separation science, 45(17).

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