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Aqua green fluorescent reactive dye

Manufactured by Thermo Fisher Scientific
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The Aqua green-fluorescent reactive dye is a fluorescent dye designed for use in various laboratory applications. It is optimized to produce a bright green fluorescent signal when excited at the appropriate wavelength. The core function of this dye is to provide a reliable and consistent fluorescent label for molecules, cells, or other samples being analyzed or imaged.

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Lab products found in correlation

Bv610, by Beckman Coulter (1 mentions)

Spelling variants of this product

Fluorescent dyes (25 citations) Aqua fluorescent reactive dye (17 citations) Aqua dye (13 citations) Aqua Fluorescent Reactive (2 citations) Aqua green (2 citations)

2 protocols using aqua green fluorescent reactive dye

1

Multiparameter Flow Cytometry for SIV Immunity

Cited 1 time
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SIV-specific cellular immune responses were assessed by IFN-γ ELISPOT assays and multiparameter ICS assays essentially as previously described9 (link). 12-color ICS assays were performed with the Aqua green-fluorescent reactive dye (Invitrogen, L23101) and predetermined titers of mAbs (Becton-Dickinson) against CD3 (SP34; Alexa Fluor 700), CD4 (OKT4; BV711, Biolegend), CD8 (SK1; allophycocyanin-cyanine 7 [APC-Cy7]), CD28 (L293; BV610), CD95 (DX2; allophycocyanin [APC]), CD69 (TP1.55.3; phycoerythrin-Texas red [energy-coupled dye; ECD]; Beckman Coulter), gamma interferon (IFN-γ) (B27; phycoerythrin-cyanine 7 [PE-Cy7]), Ki67 (B56; fluorescein isothiocyanate [FITC]), CCR5 (3A9; phycoerythrin [PE]), CCR7(3D12; Pacific Blue), and PD-1(EH21.1; peridinin chlorophyll-A-cyanine 5.5 [PerCP-Cy5.5]). IFN-γ backgrounds were consistently <0.01% in PBMC.
Whitney J.B., Hill A.L., Sanisetty S., Penaloza-MacMaster P., Liu J., Shetty M., Parenteau L., Cabral C., Shields J., Blackmore S., Smith J.Y., Brinkman A.L., Peter L.E., Mathew S.I., Smith K.M., Borducchi E.N., Rosenbloom D.I., Lewis M.G., Hattersley J., Li B., Hesselgesser J., Geleziunas R., Robb M.L., Kim J.H., Michael N.L, & Barouch D.H. (2014). Rapid Seeding of the Viral Reservoir Prior to SIV Viremia in Rhesus Monkeys. Nature, 512(7512), 74-77.
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2

Multiparameter Flow Cytometry Analysis of Immune Responses

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
12-color ICS assays shown in Figure 3D were performed essentially as described (Barouch et al., 2016 (link)) in response to stimulation with prM, Env, Cap, and NS1 peptide pools (JPT, Berlin, Germany) using the Aqua green-fluorescent reactive dye (Invitrogen, L23101) and predetermined titers of mAbs (Becton-Dickinson) against CD3 (SP34; Alexa Fluor 700), CD4 (OKT4; BV711, Biolegend), CD8 (SK1; allophycocyanin-cyanine 7 [APC-Cy7]), CD28 (L293; BV510), CD95 (DX2; allophycocyanin [APC]), CD69 (TP1.55.3; phycoerythrin-Texas red [energy-coupled dye; ECD]; Beckman Coulter), gamma interferon (IFN-γ) (B27; phycoerythrin-cyanine 7 [PE-Cy7]), Ki67 (B56; fluorescein isothiocyanate [FITC]), CCR5 (3A9; phycoerythrin [PE]), CCR7(3D12; BV421), and PD-1(EH21.1; peridinin chlorophyll-A-cyanine 5.5 [PerCP-Cy5.5]). IFN-γ backgrounds were consistently <0.01% in PBMC.
Aid M., Abbink P., Larocca R.A., Boyd M., Nityanandam R., Nanayakkara O., Martinot A.J., Moseley E.T., Blass E., Borducchi E.N., Chandrashekar A., Brinkman A.L., Molloy K., Jetton D., Tartaglia L.J., Liu J., Best K., Perelson A.S., De La Barrera R.A., Lewis M.G, & Barouch D.H. (2017). Zika Virus Persistence in the Central Nervous System and Lymph Nodes of Rhesus Monkeys. Cell, 169(4), 610-620.e14.
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