The migration and invasion of CRC cells were examined using polycarbonate transwell filters containing 8 μm pores (Becton Dickinson Labware, Franklin Lakes, NJ, USA). After treatment, the cells were seeded in serum-free media on the upper side of a transwell chamber that was either uncoated, for the migration assay, or coated with Matrigel (BD Biosciences, Bedford, MA, USA), for the invasion assay. The cells were allowed to migrate toward media containing 10% fetal bovine serum for 24 h. After the incubation period, the cells on the lower side of the membrane were fixed, stained with crystal violet, and counted. The migration and invasion indices were calculated as the mean number of cells in 10 random fields at × 20 magnification.
Polycarbonate transwell filters
Manufactured by BD
Sourced in United States
Polycarbonate transwell filters are a type of laboratory equipment used for cell culture applications. They consist of a porous polycarbonate membrane that allows for the separation and study of different cell populations in a shared culture environment.
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Lab products found in correlation
2 protocols using polycarbonate transwell filters
1
CRC Cell Migration and Invasion
2
Cell Migration Assay Protocol
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Cell growth was assessed by counting the number of cells with a hematocytometer or by the Cell Titer-Glo assay (Promega, Madison, WI, USA). Cell migration was examined using 8 μm pore size polycarbonate transwell filters (Becton Dickinson). After serum starvation for 48 h, the cells were seeded in serum-free media on the upper side of a transwell chamber and allowed to migrate towards media containing 10% FBS in the lower chamber for 24 h. After migration, the cells on the lower side of the membrane were fixed, stained with Diff-Quick stain (Sysmex, Kobe, Japan), and counted. The migration indices were calculated as the mean number of cells in 5 random fields at 20× magnification.
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