Yeast peptone dextrose medium

Manufactured by Merck Group

Sourced in United Kingdom, United States

Yeast peptone dextrose (YPD) medium is a commonly used growth medium for cultivating yeast cells. It contains yeast extract, peptone, and dextrose (glucose) as the primary components, providing essential nutrients and energy sources for optimal yeast growth and proliferation.

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Lab products found in correlation

Rpmi 1640, by Merck Group (3 mentions) Microbank vial, by Pro-Lab Diagnostics (3 mentions) S c easycomp transformation kit, by Thermo Fisher Scientific (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Qiaamp dna mini kit, by Qiagen (1 mentions) Bamhi, by Thermo Fisher Scientific (1 mentions) Apoptosis detection kit, by Merck Group (1 mentions) Cdna synthesis kit, by Roche (1 mentions) Ni nta agarose, by Qiagen (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Dithiothreitol (dtt), by Thermo Fisher Scientific (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions) Iodoacetamide, by Thermo Fisher Scientific (1 mentions) Trypan blue, by Merck Group (1 mentions) Trizol, by Merck Group (1 mentions) Hindiii, by Thermo Fisher Scientific (1 mentions) Rnase a, by Merck Group (1 mentions) Xhoi, by Thermo Fisher Scientific (1 mentions) Caprylic acid, by Merck Group (1 mentions) Giemsa dye, by Merck Group (1 mentions)

Spelling variants of this product

Dextrose (252 citations) Peptone (200 citations) Yeast peptone dextrose (8 citations) Yeast extract-peptone-dextrose (YPD) medium (3 citations) Yeast Extract-Peptone-Dextrose medium (2 citations)

7 protocols using yeast peptone dextrose medium

Yeast Strain Transformation and Growth

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Yeast strain 31019b was kindly provided to us by Prof. Brent N. Kaiser from the University of Sydney. The S.c. EasyComp Transformation Kit (#K505001) (Thermo Fisher Scientific, Waltham, Massachusetts, US) was used to make transformation competent 31019b cells. The following primers were used to PCR amplify eGFP and ChloR coding region fragments.
The coding regions were ligated into the yeast expression vector pYES2.1 using a TOPO ligation kit (#K415001) (Thermo Fisher Scientific, Waltham, Massachusetts, US).
Yeast cells were grown in Yeast Peptone Dextrose medium (#Y1375) (Sigma-Aldrich, St. Louis, Missouri, US) or Yeast Nitrogenous Base medium (#233520) (BD, Franklin Lakes, New Jersey, US) with 2% glucose for culture propagation at 30 °C. Experimental cultures were grown in YNB medium enriched with 2% galactose with various additives such as uracil (final concentration 20 μg/ml) or chloramphenicol (final concentration 4 mg/ml) depending on the experiment. Yeast growth curve standards were made using a haemocytometer and measuring the corresponding absorbance value at OD600 in a 1 cm cuvette using a Nanodrop 200c spectrophotometer (Thermo Fisher Scientific, Waltham, Massachusetts, US).

Chen J.E., Barbrook A.C., Cui G., Howe C.J, & Aranda M. (2019). The genetic intractability of Symbiodinium microadriaticum to standard algal transformation methods. PLoS ONE, 14(2), e0211936.

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Comprehensive Cell-Based Assay Protocol

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M-199, RPMI-1640, yeast peptone dextrose medium, Giemsa dye, Trypan blue, MTT, 2′,7′-dichlorodihydrofluorescein diacetate dye, N-acetyl l-cysteine (NAC), R-mevalonate lithium salt, phosphoenolpyruvate, lactate dehydrogenase, pyruvate kinase, β-NADH, ATP, glycine, diphenyleneiodonium chloride, ammonium bicarbonate, urea, sodium chloride, magnesium chloride, manganese chloride, zinc chloride, calcium chloride, copper sulphate, proteinase K, ergosterol (ERG), diphenyl hexatriene (DPH), RNase A, TRIZOL, nitro blue tetrazolium, the lactate dehydrogenase (LDH) assay kit, caprylic acid, 8-amino caprylic acid, 4-methyl octanoic acid, methyl octanoate, valproic acid, the Apoptosis Detection Kit, Taq Polymerase for PCR, Anti-His primary antibody and IgG-HRP conjugated secondary antibody and all solvents were from Sigma-Aldrich Co. (St Louis, MO, USA). The QIAamp DNA Mini kit, RNeasy Mini Kit, High fidelity Taq DNA Polymerase, and Ni-NTA agarose were from Qiagen. from Thermo-Fisher. The cDNA synthesis kit was from Hoffmann-La Roche (Basel, Switzerland). Trypsin, iodoacetamide, and DTT, restriction enzymes like BamHI, HindIII, and XhoI were purchased from Thermo Fischer (Waltham, MA, United States). The RAW 264.1 and THP-1 cell line was obtained commercially from the National Cell Repository, NCCS, Pune.

Prasad S.R., Kumar P., Mandal S., Mohan A., Chaurasia R., Shrivastava A., Nikhil P., Aishwarya D., Ramalingam P., Gajbhiye R., Singh S., Dasgupta A., Chourasia M., Ravichandiran V., Das P, & Mandal D. (2022). Mechanistic insight into the role of mevalonate kinase by a natural fatty acid-mediated killing of Leishmania donovani. Scientific Reports, 12, 16453.

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Candida albicans Adhesion Dynamics

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C. albicans SC5314 was propagated in yeast-peptone-dextrose medium (Sigma-Aldrich) for 18 h at 30 °C in an orbital shaker at 150 rpm. The cells were washed in sterile phosphate buffered saline (PBS, Sigma-Aldrich, UK) and standardised to an inoculum density of 1×10 6 CFU/ml in RPMI-1640 medium (Sigma-Aldrich, UK). The nano-imprinted sections were distributed in the appropriate wells plates (Costar, Corning Incorporated, USA), and inoculated with cells allowed to adhere for 30 and 90 min at 37 о C. These sections were then washed with PBS and retained cells removed through sonication at 35 kHz for 10 min (Ultrasonic bath, Fisher scientific, UK), followed by 15 sec vortexing. DNA and RNA was extracted using a combination of mechanical (disruption with 0.5mm glass beads) and chemical methods (TRIzol TM , Invitrogen, Paisley, UK). 20 The adherent cells were quantified using qPCR through amplification of the Candida-specific 18S DNA. 5 The expression of specific C. albicans adhesion genes (ALS1, ALS3 and EAP1) was also investigated at each exprimental parameter using real-time qPCR. 20

Alalwan H., Nile C.J., Rajendran R., McKerlie R., Reynolds P., Gadegaard N, & Ramage G. (2018). Nanoimprinting of biomedical polymers reduces candidal physical adhesion. Nanomedicine : nanotechnology, biology, and medicine, 14(3).

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Biofilm Formation in Candida albicans

Cited 2 times

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This study utilised Candida albicans strain SC5314 and a series of routine patient anonymised clinical bloodstream isolates (n = 30) collected under the approval of the NHS Scotland Caldicott Gaurdians, as part of candidaemia epidemiology surveillance study17 (link)36 (link). C. albicans clinical bloodstream isolates previously identified to have high biofilm formation (HBF [n = 15]) and low biofilm formation (LBF [n = 15]) were used throughout this study17 (link)36 (link). Isolates were stored in Microbank^® vials (Pro-Lab Diagnostics, Cheshire, UK) at −80 °C until sub-cultured onto Sabouraud’s dextrose agar (SAB [Sigma-Aldrich, Dorset, UK]). Isolates were propagated in yeast peptone dextrose (YPD) medium (Sigma-Aldrich, Dorset, UK), washed by centrifugation and re-suspended in RPMI-1640 (Sigma-Aldrich, Dorset, UK) to 1 × 10⁶ cells/mL, as described previously37 (link). Biofilms were grown in polystyrene plates or 75 cm² tissue culture flasks in RPMI for 24 h at 37 °C.

Rajendran R., May A., Sherry L., Kean R., Williams C., Jones B.L., Burgess K.V., Heringa J., Abeln S., Brandt B.W., Munro C.A, & Ramage G. (2016). Integrating Candida albicans metabolism with biofilm heterogeneity by transcriptome mapping. Scientific Reports, 6, 35436.

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Candida albicans Bloodstream Isolation

Cited 2 times

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This study utilised clinical Candida albicans (n = 60) bloodstream isolates, collected under the approval of the NHS Scotland Caldicott Gaurdian's [3 (link),24 (link)]. Isolates were stored in Microbank® vials (Pro-Lab Diagnostics, Cheshire, UK) at −80 °C until sub-cultured onto Sabouraud's dextrose agar (SAB [Sigma-Aldrich, Dorset, UK]). C. albicans isolates were propagated in yeast peptone dextrose (YPD) medium (Sigma-Aldrich, Dorset, UK), washed by centrifugation and re-suspended in RPMI-1640 with 2% D-glucose (RPMI-1640, Sigma-Aldrich, Dorset, UK) to 1 × 10⁶ cells/mL, as described previously [19 (link)].

Delaney C., Short B., Rajendran R., Kean R., Burgess K., Williams C., Munro C.A, & Ramage G. (2023). An integrated transcriptomic and metabolomic approach to investigate the heterogeneous Candida albicans biofilm phenotype. Biofilm, 5, 100112.

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Candida albicans Bloodstream Isolates Protocol

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Candida albicans SC5314, 3153A and a series of routine patient anonymised clinical bloodstream isolates (n = 42) collected under the approval of the NHS Scotland Caldicott Gaurdians from the Royal Hospital for Sick Children (Yorkhill Division), Glasgow, UK, as part of candidaemia epidemiology surveillance study. All clinical isolates obtained during this period were independently identified using Colorex Candida chromogenic plates (E&O Laboratories Ltd, Bonnybridge, UK) and were stored in Microbank® vials (Pro-Lab Diagnostics, Cheshire, UK) at −80°C until further use. These isolates were sub-cultured onto Sabouraud’s dextrose agar (SAB [Sigma-Aldrich, Dorset, UK]). Plates were incubated at 30°C for 48 h and maintained at 4°C. Isolates were propagated in yeast peptone dextrose (YPD) medium (Sigma-Aldrich, Dorset, UK), washed by centrifugation and resuspended in the appropriate media (Sigma-Aldrich) to the desired concentration, as described previously [50 (link)].

Sherry L., Rajendran R., Lappin D.F., Borghi E., Perdoni F., Falleni M., Tosi D., Smith K., Williams C., Jones B., Nile C.J, & Ramage G. (2014). Biofilms formed by Candida albicans bloodstream isolates display phenotypic and transcriptional heterogeneity that are associated with resistance and pathogenicity. BMC Microbiology, 14, 182.

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Candida albicans Biofilm Characterization

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Candida albicans DAY 185 was used in the experiments. Three clinical isolates of C. albicans, named C. albicans BF1, BF2, and BF3, were obtained from the Medical University of Vienna. Each experiment was repeated three times. All of them are able to form strong biofilms, which have been confirmed by the crystal violet method. The strains were cultured in a Yeast Peptone Dextrose (YPD) medium (Sigma-Aldrich, Austria) at 30°C. RPMI 1640 medium (Thermo Fisher Scientific, Waltham, MA, United States) was used for biofilm growth. Gls (lyticase from Arthrobacter luteus, ≥2,000 units/mg protein), CS (low molecular weight, degree of deacetylation 75–85%), AMB, and other reagents were purchased from Sigma-Aldrich.

Tan Y., Ma S., Ding T., Ludwig R., Lee J, & Xu J. (2022). Enhancing the Antibiofilm Activity of β-1,3-Glucanase-Functionalized Nanoparticles Loaded With Amphotericin B Against Candida albicans Biofilm. Frontiers in Microbiology, 13, 815091.

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