BALB/C and C57BL/6J mice (female, 6–8-week-old) were purchased from Daehan Biolink (Eumseong, Korea). All animal procedures were approved by the Institutional Animal Care and Use Committee (IACUC) of Chungnam National University (CNU-01056). The murine colon cancer CT26 and the B16F10 melanoma cell lines were cultured in RPMI-1640 (Welgene, Gyeongsan, Korea) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Sigma-Aldrich, St. Louis, MO, USA), 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin (Sigma-Aldrich) in a humidified 5% CO2 atmosphere at 37 °C. Sodium butyrate (10 mM, 303410, Sigma-Aldrich) was used to induce baculovirus infection and expression of transgenes. SF9 insect cells were maintained in Sf-900II SFM (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 5% FBS.
Balb c
Manufactured by Daehan Biolink
BALB/C is a strain of laboratory mouse commonly used in immunological and cancer research. It is a well-established and widely-used mouse model.
Automatically generated - may contain errors
Lab products found in correlation
Sodium butyrate, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Penicillin, by Merck Group (1 mentions)
Sf 900 2 sfm, by Thermo Fisher Scientific (1 mentions)
C57bl 6j mice, by Daehan Biolink (1 mentions)
Rpmi 1640, by Welgene (1 mentions)
Acetylsalicylic acid, by Merck Group (1 mentions)
Sodium carboxyl methylcellulose, by Merck Group (1 mentions)
2 4 dinitrochlorobenzene, by Merck Group (1 mentions)
C57bl 6, by Daehan Biolink (1 mentions)
Ap1 luciferase construct, by Addgene (1 mentions)
Hek293, by American Type Culture Collection (1 mentions)
Raw264, by American Type Culture Collection (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Lps e coli 0111 b4, by Merck Group (1 mentions)
D galn, by Merck Group (1 mentions)
Phorbol 12 myristate, by Merck Group (1 mentions)
C57bl 6 mice, by Daehan Biolink (1 mentions)
3 protocols using balb c
1
Murine Cancer Cell Culture Protocol
2
Animal Handling and Reagent Procurement for Mouse Studies
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Six-week-old ICR, Balb/c, and C57BL/6 male mice (see Table S2 for genetic background information) were purchased from Daehan Biolink (DBL, Chungbuk, Korea) and housed eight mice per group under a 12-h light/dark cycle (lights on at 6 a.m.). Water and a pellet diet (Samyang, Daejeon, Korea) were supplied ad libitum. Animal care followed guidelines issued by the National Institutes of Health for the Care and Use of Laboratory Animals (NIH Publication 80-23, revised in 1996) and the Institutional Animal Care and Use Committee at Sungkyunkwan University (Approval No.: SKKUIACUC-2016-01-0002-2). Phorbol-12-myristate, sodium carboxyl methylcellulose, 2,4-dinitrochlorobenzene (DNCB), dextran sodium sulphate (DSS), acetylsalicylic acid, D-GalN, LPS (E. coli 0111:B4), 100% EtOH, and HCl were purchased from Sigma Chemical Co. (St. Louis, MO, USA). MAPK inhibitors (SB203580, SP600125, and U0126) were purchased from Calbiochem (La Jolla, CA, USA). RAW264.7, HEK293, and MDA-MB-231 cells were purchased from the American Type Culture Collection (Manassas, VA, USA). Detailed information on antibodies used in this study is explained in Supplementary Materials . The AP-1 luciferase construct was purchased from Addgene (Cambridge, MA, USA).
3
MERS and HPV Vaccine Immunization in Mice
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For immunization with MERS S soluble protein vaccine and HPV L1 virus-like particle (VLP) vaccine, female BALB/c and C57BL/6 mice were purchased from Dae-Han Bio-Link. Female TLR7 KO mice were kindly provided by Dr. Bumseok Kim, Chonbuk National University, and hDPP4 Tg mice, stably expressing hDPP4 as the MERS-CoV receptor, were kindly provided by Dr. Sungkyun Park, Korea Research Institute of Bioscience & Biotechnology. Mice were housed at the Catholic University of Korea under specific-pathogen-free conditions with 12-h light/dark cycle and handled according to protocols approved by the Catholic University of Korea. The animal facility at the Catholic University of Korea is fully accredited by the Korean Association for Laboratory Animals. All mice experimental procedures conducted in this study followed the guidelines of the Institutional Animal Care and Use Committee of the Catholic University of Korea (CUK-IACUC-2016-039, 2016-046, 2017-028, 2018-027, and 2018-028).
For MERS-CoV challenge, hDPP4-transgenic (Tg) mice (5–7-weeks old) were maintained in a biosafety level 3 facility at the Korea Zoonosis Research Institute of Chonbuk National University and followed the guidelines of the Institutional Animal Care and Use Committee (2018-045).
For MERS-CoV challenge, hDPP4-transgenic (Tg) mice (5–7-weeks old) were maintained in a biosafety level 3 facility at the Korea Zoonosis Research Institute of Chonbuk National University and followed the guidelines of the Institutional Animal Care and Use Committee (2018-045).
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